In:
Microbial Biotechnology, Wiley, Vol. 11, No. 6 ( 2018-11), p. 1121-1123
Abstract:
Sepsis, a life‐threatening systemic infection, requires quick treatment. Gram‐negative bacteria ( GNB ) are the major causative pathogens and their endotoxin can be a surrogate biomarker for diagnosis. We explored a fast identification of GNB by first culturing blood to increase endotoxin levels and then detecting endotoxin by Tachypleus amebocyte lysate ( TAL ) with kinetic turbidimetric assay ( KT ‐ TAL ). Heating samples could significantly increase the endotoxin released from GNB ; speed and time of centrifugation, and sample dilution could affect the endotoxin results. At a high GNB load, endotoxin was detected 3 h after culture, 6.5 h earlier than the BD BACTEC blood culture system detecting GNB . At a low GNB load, endotoxin was detected at 9 h after culture, 13 h earlier than by the BD BACTEC system. In a sepsis patient with Acinetobacter baumannii, we detected endotoxin at 12 h after culture, while the BD BACTEC system needed 28.5 h for detection, allowing physicians an earlier decision on appropriate treatment.
Type of Medium:
Online Resource
ISSN:
1751-7915
,
1751-7915
DOI:
10.1111/mbt2.2018.11.issue-6
DOI:
10.1111/1751-7915.13314
Language:
English
Publisher:
Wiley
Publication Date:
2018
detail.hit.zdb_id:
2406063-X
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