In:
Phytotherapy Research, Wiley, Vol. 32, No. 12 ( 2018-12), p. 2475-2479
Abstract:
This study aimed to investigate in vitro the anti‐influenza B/Lee/40 virus effect of sakuranetin and mode of its action. The sakuranetin exhibited potent antiviral activity against influenza B/Lee/40 virus, reducing the formation of a visible cytopathic effect, with a 50% inhibitory concentration (IC 50 ) of 7.21 μg/ml and no cytotoxicity at a concentration of 100 μg/ml, and the derived therapeutic index (TI) was 〉 13.87. Oseltamivir showed weak anti‐influenza B/Lee/40 virus activity with IC 50 of 80.74 μg/ml, 50% cytotoxicity concentration of 〉 100 μg/ml, and TI of 〉 1.24. Sakuranetin also showed effective inhibitory effects when added at the viral attachment, entry, and postentry steps. Moreover, sakuranetin effectively inactivated influenza B/Lee/40 virus infection in dose‐ and temperature‐dependent manners. Sakuranetin indicated an inhibitory effect in viral RNA synthesis in the presence of 100 μg/ml of sakuranetin. Overall, this research revealed that sakuranetin could inhibit influenza B/Lee/40 virus replication and that sakuranetin may be involved in the virus attachment, entry, and postentry. Therefore, sakuranetin is a good candidate for a chemopreventive agent for influenza virus‐related diseases.
Type of Medium:
Online Resource
ISSN:
0951-418X
,
1099-1573
Language:
English
Publisher:
Wiley
Publication Date:
2018
detail.hit.zdb_id:
1493490-5
SSG:
15,3
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