In:
The FEBS Journal, Wiley, Vol. 282, No. 8 ( 2015-04), p. 1512-1525
Abstract:
Micro RNA s (mi RNA s) are small non‐coding RNA molecules that regulate gene expression at the transcriptional and post‐transcriptional levels. Here we show that miR‐30e, which was previously identified as an ionizing radiation‐inducible mi RNA , enhances cellular invasion by promoting secretion of the matrix metalloproteinase MMP ‐2. The enhancement of cellular invasion by miR‐30e involved up‐regulation of the epidermal growth factor receptor ( EGFR ) and subsequent activation of its downstream signaling mediators, AKT and extracellular signal‐regulated kinase . EGFR up‐regulation by miR‐30e occurred due to stabilization of the EGFR protein. The E3 ubiquitin ligase casitas B‐lineage lymphoma B ( CBL ‐B) was down‐regulated by miR‐30e, and this led to increased EGFR abundance. A 3′ UTR reporter assay confirmed that CBL ‐B is a direct target of miR‐30e. Knocking down CBL ‐B expression phenocopied the effects of miR‐30e, whereas ectopic expression of CBL ‐B suppressed miR‐30e‐induced EGFR up‐regulation and invasion. Collectively, our results suggest that targeting miR‐30e may limit the invasiveness induced during glioma radiotherapy.
Type of Medium:
Online Resource
ISSN:
1742-464X
,
1742-4658
DOI:
10.1111/febs.2015.282.issue-8
Language:
English
Publisher:
Wiley
Publication Date:
2015
detail.hit.zdb_id:
2172518-4
SSG:
12
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