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  • 1
    Online Resource
    Online Resource
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2016
    In:  Vietnam Journal of Biotechnology Vol. 14, No. 2 ( 2016-06-30), p. 369-375
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 14, No. 2 ( 2016-06-30), p. 369-375
    Abstract: Acid mine drainage (AMD) is contaminated water from mining indistry, characterized by low pH (1 – 4) and high concentration of heavy metals (up to thousands ppm). AMD is highly toxic to aquatic life and soil ecology surrounding the mining areas, therefore should be treated adequately before discharging to the environment. The treatment technology based on sulfate-reducing bioreactors has been applying widely with high efficiency. Sulfate-reducing bacteria (SRB) stand at the central point of the technology, use hydrogen and organic carbons to reduce sulfate to sulfde, that involve in metal precipitation and pH neutralization. For establishing the technology, sources of SRB as well as organic substrates neccesary for the bacteria should be acquired from outside. In many cases, these two requirements can be supplied from cow manure and agriculture residues (such as rice straws) added to the bioreactor before operating. In the present study, a mixed culture of SRB enriched from aquaculture-processing wastewater was used to start up the sulfate-reducing bioreactor for the AMD-treatment laboratory model. Cotreatment of AMD and poultry wastewater in this model operated under continuous mode with retention time of 48 h allowed to remove 85 – 88% Fe2+ in the AMD (from the original concentration of 200 mg/L). Study of the bacterial community via DGGE analyses of the 16S rDNA fragments showed that the enrichment culture consisted of three main SRB genera Desulfovibrio, Desulfomicrobium and Desulfobulbus spp., whereas in the sediment of the bioreactor only Desulfovibrio spp were found dominating. The obtained results would serve as basis for the development of biological-based technology to treat AMD together with organic-rich wastewater sources, suitable for mines located closely to residential areas.
    Type of Medium: Online Resource
    ISSN: 1811-4989 , 1811-4989
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2016
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  • 2
    Online Resource
    Online Resource
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2016
    In:  Vietnam Journal of Biotechnology Vol. 14, No. 3 ( 2016-09-30), p. 581-588
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 14, No. 3 ( 2016-09-30), p. 581-588
    Abstract: Single cell protein (SCP) can be produced from biomass of different types of microorganisms that have high protein content such as yeast, filamentous fungi, algae and bacteria. In comparison to animal and plant protein sources, this kind of protein has several advantages, namely high protein and nutrient contents, being produced in fermenters with the use of variety of organic wastes, independence in agriculture land or climate conditions. Methane oxidizing bacteria (MOB) are considered as good candidates for SCP production and have been intensively studied recently. In the present study, a MOB strain BG3 was isolated from wastewater of an anaerobic digester via enrichment and isolation procedures using methane as the only carbon and energy sources. Strain BG3 comprised of oval-shaped cells of 0,8-1´16 -1,8 μm in size, almost nonmotile. Based on comparative analyses of the 16S rDNA partial sequences, strain BG3 was identified as a member of the Methylomonas genus, the most closely related species was Methylomonas koyamae (97% homology). This was also confirmed by analyses of sequence of the pmoA gene, encoding for a-subunit in the methane-monooxygenase in the strain.  Besides methane, strain BG3 also utilized methanol for the growth. It has been shown that methane-fed culture of strain BG3 could produce 68.69 g crude protein per 100 g CDW and the according methane to biomass conversion efficiency was 2,8 m3 methane×kg-1 dry biomassas. Owing the capability of utilization of methane, the second important greenhouse gas, for the production of protein source for animal feed, strain BG3 would have a great application potential in Vietnam. Strain BG3 was designated as Methylomonas sp. BG3 and its 16S rDNA and pmoA gene sequences were deposited at the GenBank with accession numbers of KJ081955 and KJ081956, respectively.
    Type of Medium: Online Resource
    ISSN: 1811-4989 , 1811-4989
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2016
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  • 3
    Online Resource
    Online Resource
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2018
    In:  Vietnam Journal of Biotechnology Vol. 14, No. 4 ( 2018-04-19), p. 777-784
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 14, No. 4 ( 2018-04-19), p. 777-784
    Abstract: A pilot-scale system of a total volume of 6 m3 using sulfate reducing (SR) bioreactor technology was established for the treatment of acidic wastewater from Thien Ke tin processing factory in Tuyen Quang province, Vietnam. In the system, the acidic wastewater with high metal content went first to a collecting tank filled with limestone gravels to increase pH to a value favorable for SRB growth, and at the second step to a SR bioreactor where sulfate reduction occurred to produce sulfide for metal precipitation. To activate the SR bioreactor, a laboratory SRB mixed culture dominated by Desufovibiro, Desulfobulbus and Desulfomicrobium species was added at a cell density of 106 cell/ml so that a full activation was achieved just after a week of incubation. Molasses was added to the SR bioreactor at 0.5 ml/L as substrate for the SRB growth during the operation. The performance of the system was studied under batch and continuous modes. The batch mode showed good results after three day-operation. The pH increased from 2.8 – 3.2 to 7 – 7.2, and a total of 750 mg/L sulfate was reduced to sulfide presumably by the SRB. The produced sulfide efficiently removed metals from the wastewater, such as iron from 143.1 mg/L to 0.3 mg/L, copper from 16.32 mg/L to 0.04 mg/L and manganese from 10.9 mg/L to 0.05 mg/L. The continuous mode with a hydraulic load of 100 l/h and an according retention time of three days showed constitutive contaminant removal. The effluent pH of the system was around 7 within six-day period. The sulfate reduction was active, keeping sulfate concentration in the final effluent as low as  150 mg/L. Accordingly, the three most metal contaminants (iron, copper and manganese) were found at concentrations below the regulated limits. The results showed the possibility of applying SR bioreactor technology for the treatment of AMD is feasible and the use of previously enriched mixed culture of SRB could be a good approach to shorten the activation period of the SR bioreactor.
    Type of Medium: Online Resource
    ISSN: 1811-4989 , 1811-4989
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2018
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  • 4
    In: Microbial Biotechnology, Wiley, Vol. 8, No. 3 ( 2015-05), p. 579-589
    Abstract: In this study, we attempted to enrich neutrophilic iron bacteria in a microbial fuel cell ( MFC )‐type reactor in order to develop a lithotrophic MFC system that can utilize ferrous iron as an inorganic electron donor and operate at neutral pHs . Electrical currents were steadily generated at an average level of 0.6 mA (or 0.024 mA cm –2 of membrane area) in reactors initially inoculated with microbial sources and operated with 20 mM Fe 2+ as the sole electron donor and 10 ohm external resistance; whereas in an uninoculated reactor (the control), the average current level only reached 0.2 mA (or 0.008 mA cm –2 of membrane area). In an inoculated MFC , the generation of electrical currents was correlated with increases in cell density of bacteria in the anode suspension and coupled with the oxidation of ferrous iron. Cultivation‐based and denaturing gradient gel electrophoresis analyses both show the dominance of some P seudomonas species in the anode communities of the MFCs . Fluorescent in‐situ hybridization results revealed significant increases of neutrophilic iron‐oxidizing bacteria in the anode community of an inoculated MFC . The results, altogether, prove the successful development of a lithotrophic MFC system with iron bacteria enriched at its anode and suggest a chemolithotrophic anode reaction involving some P seudomonas species as key players in such a system. The system potentially offers unique applications, such as accelerated bioremediation or on‐site biodetection of iron and/or manganese in water samples.
    Type of Medium: Online Resource
    ISSN: 1751-7915 , 1751-7915
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2015
    detail.hit.zdb_id: 2406063-X
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  • 5
    Online Resource
    Online Resource
    Centre of Sociological Research, NGO ; 2019
    In:  Journal of International Studies Vol. 12, No. 1 ( 2019-3), p. 229-238
    In: Journal of International Studies, Centre of Sociological Research, NGO, Vol. 12, No. 1 ( 2019-3), p. 229-238
    Type of Medium: Online Resource
    ISSN: 2071-8330 , 2306-3483
    Language: Unknown
    Publisher: Centre of Sociological Research, NGO
    Publication Date: 2019
    detail.hit.zdb_id: 2859533-6
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  • 6
    Online Resource
    Online Resource
    Centre of Sociological Research, NGO ; 2017
    In:  Journal of International Studies Vol. 10, No. 4 ( 2017-12), p. 192-206
    In: Journal of International Studies, Centre of Sociological Research, NGO, Vol. 10, No. 4 ( 2017-12), p. 192-206
    Type of Medium: Online Resource
    ISSN: 2071-8330 , 2306-3483
    Language: Unknown
    Publisher: Centre of Sociological Research, NGO
    Publication Date: 2017
    detail.hit.zdb_id: 2859533-6
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  • 7
    Online Resource
    Online Resource
    Centre of Sociological Research, NGO ; 2016
    In:  Journal of International Studies Vol. 9, No. 3 ( 2016-11), p. 124-137
    In: Journal of International Studies, Centre of Sociological Research, NGO, Vol. 9, No. 3 ( 2016-11), p. 124-137
    Type of Medium: Online Resource
    ISSN: 2071-8330 , 2306-3483
    Language: Unknown
    Publisher: Centre of Sociological Research, NGO
    Publication Date: 2016
    detail.hit.zdb_id: 2859533-6
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  • 8
    In: Antimicrobial Resistance & Infection Control, Springer Science and Business Media LLC, Vol. 6, No. S2 ( 2017-2)
    Type of Medium: Online Resource
    ISSN: 2047-2994
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2017
    detail.hit.zdb_id: 2666706-X
    SSG: 15,3
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  • 9
    Online Resource
    Online Resource
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2017
    In:  Vietnam Journal of Science and Technology Vol. 55, No. 6 ( 2017-12-11), p. 690-
    In: Vietnam Journal of Science and Technology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 55, No. 6 ( 2017-12-11), p. 690-
    Abstract: The process of enzyme assisted extraction of essential oil from the leaves and branches of the Vietnamese aromatic plant Cinnamomum cassia was studied and optimized using a Box-Wilson central composite design consisting of 05 independent variables (pH, temperature T, time τ, concentration of the enzyme Laccase, and concentration of the enzyme Cellic Htec2) and two dependent variables (reducing sugar and yield of essential oil). Second-order polynomial equations were obtained for the responses, which fitted well with the experimental data. Optimal conditions for oil yield were found at pH = 5.2; T = 440C; τ = 5h30'; Laccase = 0.42 ml/g, and Cellic Htec2 = 1.15%. The experimental value (0.982% oil yield) was close to the predicted value (0.978%). The application of enzyme assisted extraction  in combination with optimization using response surface methodology substantially improved the oil yield as compared with traditional method. 
    Type of Medium: Online Resource
    ISSN: 2525-2518 , 2525-2518
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2017
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  • 10
    In: Journal of Clinical Virology, Elsevier BV, Vol. 66 ( 2015-05), p. 24-26
    Type of Medium: Online Resource
    ISSN: 1386-6532
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2015
    detail.hit.zdb_id: 1499932-8
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