In:
Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, Vol. 112, No. 23 ( 2015-06-09), p. 7225-7230
Abstract:
CD4 + regulatory T (Treg) cells expressing CD25 and the transcription factor forkhead box P3 (FOXP3) are indispensable for immunological self-tolerance and homeostasis. FOXP3 + CD25 + CD4 + T cells in humans, however, are heterogeneous in function and differentiation status, including suppressive or nonsuppressive cells as well as resting or activated Treg cells. We have searched for cell surface markers specific for suppression-competent Treg cells by using a panel of currently available monoclonal antibodies reactive with human T cells. We found that CD15s (sialyl Lewis x) was highly specific for activated, terminally differentiated, and most suppressive FOXP3 high effector Treg (eTreg) cells and able to differentiate them in various clinical settings from nonsuppressive FOXP3 + T cells secreting inflammatory cytokines. For example, CD15s + FOXP3 + eTreg cells were increased in sarcoidosis, whereas it was nonsuppressive CD15s − FOXP3 + T cells that were expanded in lupus flares. FOXP3 + cells induced from conventional CD4 + T cells by T-cell receptor stimulation hardly expressed CD15s. CD15s + CD4 + T-cell depletion was sufficient to evoke and enhance in vitro immune responses against tumor or viral antigens. Collectively, we have identified CD15s as a biomarker instrumental in both phenotypic and functional analysis of FOXP3 + CD4 + T-cell subpopulations in health and disease. It allows specific targeting of eTreg cells, rather than whole FOXP3 + CD4 + T cells, in controlling immune responses.
Type of Medium:
Online Resource
ISSN:
0027-8424
,
1091-6490
DOI:
10.1073/pnas.1508224112
Language:
English
Publisher:
Proceedings of the National Academy of Sciences
Publication Date:
2015
detail.hit.zdb_id:
209104-5
detail.hit.zdb_id:
1461794-8
SSG:
11
SSG:
12
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