In:
eLife, eLife Sciences Publications, Ltd, Vol. 6 ( 2017-12-04)
Abstract:
Neurosecretory centers in animal brains use peptidergic signaling to influence physiology and behavior. Understanding neurosecretory center function requires mapping cell types, synapses, and peptidergic networks. Here we use transmission electron microscopy and gene expression mapping to analyze the synaptic and peptidergic connectome of an entire neurosecretory center. We reconstructed 78 neurosecretory neurons and mapped their synaptic connectivity in the brain of larval Platynereis dumerilii, a marine annelid. These neurons form an anterior neurosecretory center expressing many neuropeptides, including hypothalamic peptide orthologs and their receptors. Analysis of peptide-receptor pairs in spatially mapped single-cell transcriptome data revealed sparsely connected networks linking specific neuronal subsets. We experimentally analyzed one peptide-receptor pair and found that a neuropeptide can couple neurosecretory and synaptic brain signaling. Our study uncovered extensive networks of peptidergic signaling within a neurosecretory center and its connection to the synaptic brain.
Type of Medium:
Online Resource
ISSN:
2050-084X
DOI:
10.7554/eLife.26349.001
DOI:
10.7554/eLife.26349.002
DOI:
10.7554/eLife.26349.012
DOI:
10.7554/eLife.26349.003
DOI:
10.7554/eLife.26349.004
DOI:
10.7554/eLife.26349.005
DOI:
10.7554/eLife.26349.006
DOI:
10.7554/eLife.26349.007
DOI:
10.7554/eLife.26349.008
DOI:
10.7554/eLife.26349.009
DOI:
10.7554/eLife.26349.010
DOI:
10.7554/eLife.26349.011
DOI:
10.7554/eLife.26349.013
DOI:
10.7554/eLife.26349.014
DOI:
10.7554/eLife.26349.015
DOI:
10.7554/eLife.26349.016
DOI:
10.7554/eLife.26349.026
DOI:
10.7554/eLife.26349.027
DOI:
10.7554/eLife.26349.028
DOI:
10.7554/eLife.26349.029
DOI:
10.7554/eLife.26349.017
DOI:
10.7554/eLife.26349.018
DOI:
10.7554/eLife.26349.019
DOI:
10.7554/eLife.26349.020
DOI:
10.7554/eLife.26349.021
DOI:
10.7554/eLife.26349.022
DOI:
10.7554/eLife.26349.023
DOI:
10.7554/eLife.26349.024
DOI:
10.7554/eLife.26349.025
DOI:
10.7554/eLife.26349.030
DOI:
10.7554/eLife.26349.034
DOI:
10.7554/eLife.26349.031
DOI:
10.7554/eLife.26349.032
DOI:
10.7554/eLife.26349.033
DOI:
10.7554/eLife.26349.035
DOI:
10.7554/eLife.26349.036
DOI:
10.7554/eLife.26349.037
DOI:
10.7554/eLife.26349.041
DOI:
10.7554/eLife.26349.042
Language:
English
Publisher:
eLife Sciences Publications, Ltd
Publication Date:
2017
detail.hit.zdb_id:
2687154-3
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