In:
Journal of AOAC INTERNATIONAL, Oxford University Press (OUP), Vol. 100, No. 1 ( 2017-01-01), p. 159-164
Kurzfassung:
A liquid chip technique was developed to detect spring viremia of carp virus (SVCV), infectious hematopoietic necrosis virus (IHNV), and viral hemorrhagic septicemia virus (VHSV) of salmonids simultaneously. Sequencesof the G gene of SVCV, N gene of IHNV, and G gene of VHSV were used to design SVCV-, IHNV-, and VHSV-specific primers, which were labeled with biotin and subjected to amination modification. They were then coupled with fluorescence-coded microspheres and used for hybridization with reverse-transcription PCR products of SVCV, IHNV, and VHSV. A BD FACSArray was used to detect fluorescencesignal in the reaction system. This assay system had a high sensitivity to SVCV, VHSV, and IHNV, with LODs of 10, 10, and 100 pg/μL, respectively. Moreover, the assay was specific for the detection of SVCV, IHNV, and VHSV and was not susceptible to cross-detection of other viruses, including pike fry rhabdovirus, hirame rhabdovirus, infectious pancreatic necrosis virus, viral nervous necrosis virus, yellowtail ascites virus, grass carp reovirus, red sea bream iridovirus, and koi herpesvirus. The liquid chip assay technique established inthis study provides a novel, convenient, and rapid approach for the detection of SVCV, IHNV, and VHSV.
Materialart:
Online-Ressource
ISSN:
1060-3271
,
1944-7922
DOI:
10.5740/jaoacint.16-0066
Sprache:
Englisch
Verlag:
Oxford University Press (OUP)
Publikationsdatum:
2017
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