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  • 1
    Online Resource
    Online Resource
    Proteomic Analysis Reveals Allergen Variability among Breeds of the Dust Mite 〈b〉〈i〉Blomia tropicalis〈/i〉〈/b〉
    S. Karger AG ; 2019
    In:  International Archives of Allergy and Immunology Vol. 180, No. 3 ( 2019), p. 159-172
    Details
    In: International Archives of Allergy and Immunology, S. Karger AG, Vol. 180, No. 3 ( 2019), p. 159-172
    Abstract: 〈 b 〉 〈 i 〉 Background: 〈 /i 〉 〈 /b 〉 The dawn of the “omics” technologies has changed allergy research, increasing the knowledge and identification of new allergens. However, these studies have been almost restricted to 〈 i 〉 Dermatophagoides 〈 /i 〉 spp. Although 〈 i 〉 Blomia tropicalis 〈 /i 〉 has long been established as a clinically important source of allergens, a thorough proteomic characterization is still lacking for this dust mite. 〈 b 〉 〈 i 〉 Objective: 〈 /i 〉 〈 /b 〉 To increase knowledge of 〈 i 〉 B. tropicalis 〈 /i 〉 allergens through proteomic analysis. 〈 b 〉 〈 i 〉 Methods: 〈 /i 〉 〈 /b 〉 Eleven in-bred lineages of 〈 i 〉 B. tropicalis 〈 /i 〉 were obtained from 11 unique different pregnant females. Their somatic extracts were analyzed and compared with a commercially available extract by liquid chromatography tandem mass spectrometry (LC-MS/MS). 〈 b 〉 〈 i 〉 Results: 〈 /i 〉 〈 /b 〉 Considerable differences in the protein expression profiles were found among the breeds, and most of them displayed higher expression levels of major allergens than the commercially available extract. Blo t 2 was the most prominent allergenic protein in the analyzed extracts. Six identified allergens and 14 isoforms have not yet been recognized by IUIS. Conversely, 3 previously recognized 〈 i 〉 B. tropicalis 〈 /i 〉 allergens were not found. 〈 b 〉 〈 i 〉 Conclusions: 〈 /i 〉 〈 /b 〉 The clear impact of inbreeding on allergen content shown by our study leads us to conclude that the quantification and/or identification of allergens from in-bred lines should be routinely considered for mite cultivation in order to select breeds with higher amounts of major allergens. In this sense, LC-MS/MS may be a useful method to achieve this quality control for research and commercial purposes.
    Type of Medium: Online Resource
    ISSN: 1018-2438 , 1423-0097
    URL: Article
    DOI: 10.1159/000501964
    RVK:
    XA 49650
    Language: English
    Publisher: S. Karger AG
    Publication Date: 2019
    detail.hit.zdb_id: 1482722-0
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  • 2
    Online Resource
    Online Resource
    Chronic Glibenclamide Treatment Attenuates Walker-256 Tumour Growth in Prediabetic Obese Rats
    S. Karger AG ; 2017
    In:  Cellular Physiology and Biochemistry Vol. 42, No. 1 ( 2017), p. 81-90
    Details
    In: Cellular Physiology and Biochemistry, S. Karger AG, Vol. 42, No. 1 ( 2017), p. 81-90
    Abstract: Background/Aims: The sulphonylurea glibenclamide (Gli) is widely used in the treatment of type 2 diabetes. In addition to its antidiabetic effects, low incidences of certain types of cancer have been observed in Gli-treated diabetic patients. However, the mechanisms underlying this observation remain unclear. The aim of the present work was to evaluate whether obese adult rats that were chronically treated with an antidiabetic drug, glibenclamide, exhibit resistance to rodent breast carcinoma growth. Methods: Neonatal rats were treated with monosodium L-glutamate (MSG) to induce prediabetes. Control and MSG groups were treated with Gli (2 mg/kg body weight/day) from weaning to 100 days old. After Gli treatment, the control and MSG rats were grafted with Walker-256 tumour cells. After 14 days, grafted rats were euthanized, and tumour weight as well as glucose homeostasis were evaluated. Results: Treatment with Gli normalized tissue insulin sensitivity and glucose tolerance, suppressed fasting hyperinsulinaemia, reduced fat tissue accretion in MSG rats, and attenuated tumour growth by 27% in control and MSG rats. Conclusions: Gli treatment also resulted in a large reduction in the number of PCNA-positive tumour cells. Although treatment did improve the metabolism of pre-diabetic MSG-rats, tumour growth inhibition may be a more direct effect of glibenclamide.
    Type of Medium: Online Resource
    ISSN: 1015-8987 , 1421-9778
    URL: Article
    DOI: 10.1159/000477117
    Language: English
    Publisher: S. Karger AG
    Publication Date: 2017
    detail.hit.zdb_id: 1482056-0
    SSG: 12
    SSG: 15,3
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  • 3
    Online Resource
    Online Resource
    Particulate Matter Exposure During Perinatal Life Results in Impaired Glucose Metabolism in Adult Male Rat Offspring
    S. Karger AG ; 2018
    In:  Cellular Physiology and Biochemistry Vol. 49, No. 1 ( 2018), p. 395-405
    Details
    In: Cellular Physiology and Biochemistry, S. Karger AG, Vol. 49, No. 1 ( 2018), p. 395-405
    Abstract: Background/Aims: Particulate matter (PM) is an important risk factor for immunological system imbalance due to its small size, which can reach more distal regions of the respiratory tract, independently of its chemical composition. Some studies have suggested that PM exposure is associated with an increased incidence of diabetes, especially in industrialized urban regions. However, studies regarding the effects of PM exposure during perinatal life on glucose metabolism are limited. We tested whether exposure to PM from an urban area with poor air quality during pregnancy and lactation could cause short- and long-term dysfunction in rat offspring. Methods: Samples of 〈 10 µm PM were collected in an urban area of Cotonou, Benin (West Africa), and reconstituted in corn oil. Pregnant Wistar rats received 50 µg PM/day by gavage until the end of lactation. After birth, we analyzed the dams’ biochemical parameters as well as those of their male offspring at 21 and 90 days of age. Results: The results showed that PM exposure did not lead to several consequences in dams; however, the male offspring of both ages presented an increase of approximately 15% in body weight. Although the blood glucose levels remained unchanged, the insulin levels were increased 2.5- and 2-fold in PM exposure groups of both ages, respectively. HOMA-IR and HOMA-β were also increased at both ages. We also demonstrated that the number, islet area and insulin immunodensity of pancreatic islets were significantly increased at both ages from PM exposure. Conclusion: Our data show that chronic PM exposure by the oral route during perinatal life in rats leads to glucose dyshomeostasis in male offspring both in early and later life. Thus, we suggest that an ambience with poor air quality, mainly where traffic is dense, can contribute to an increase in metabolic disease incidence.
    Type of Medium: Online Resource
    ISSN: 1015-8987 , 1421-9778
    URL: Article
    DOI: 10.1159/000492901
    Language: English
    Publisher: S. Karger AG
    Publication Date: 2018
    detail.hit.zdb_id: 1482056-0
    SSG: 12
    SSG: 15,3
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  • 4
    Online Resource
    Online Resource
    Allergens of 〈b〉〈i〉Blomia tropicalis〈/i〉〈/b〉: An Overview of Recombinant Molecules
    S. Karger AG ; 2017
    In:  International Archives of Allergy and Immunology Vol. 172, No. 4 ( 2017), p. 203-214
    Details
    In: International Archives of Allergy and Immunology, S. Karger AG, Vol. 172, No. 4 ( 2017), p. 203-214
    Abstract: Allergic diseases are considered a major problem for healthcare systems in both developed and developing countries. House dust mites are well-known triggers of allergic manifestations. While the 〈 i 〉 Dermatophagoides 〈 /i 〉 genus is widely distributed globally, 〈 i 〉 Blomia tropicalis 〈 /i 〉 is the most prominent mite species in the tropical and subtropical regions of the world. Over the last decades, an increase in sensitization rates to 〈 i 〉 B. tropicalis 〈 /i 〉 has been reported, leading to increased research efforts on 〈 i 〉 Blomia 〈 /i 〉 allergens. In fact, 8 new allergens have been identified and characterized to different degrees. Here, we provide an overview of recent developments concerning the identification and production of recombinant 〈 i 〉 Blomia 〈 /i 〉 allergens, as well as their structural and immunological characterization. Although considerable progress has been achieved, detailed molecule-based studies are still needed to better define the clinical relevance of 〈 i 〉 Blomia 〈 /i 〉 allergens. Thus, the establishment of a well-standardized and fully characterized panel of allergens remains a challenge for the development of better diagnosis and therapy of allergic diseases induced by 〈 i 〉 B. tropicalis 〈 /i 〉 .
    Type of Medium: Online Resource
    ISSN: 1018-2438 , 1423-0097
    URL: Article
    DOI: 10.1159/000464325
    RVK:
    XA 49650
    Language: English
    Publisher: S. Karger AG
    Publication Date: 2017
    detail.hit.zdb_id: 1482722-0
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  • 5
    Online Resource
    Online Resource
    Anti-TNF Exposure during Pregnancy in Crohn’s Disease Patients
    S. Karger AG ; 2018
    In:  Case Reports in Gastroenterology Vol. 12, No. 3 ( 2018-10-17), p. 608-616
    Details
    In: Case Reports in Gastroenterology, S. Karger AG, Vol. 12, No. 3 ( 2018-10-17), p. 608-616
    Abstract: Inflammatory bowel disease (IBD) affects young people of reproductive age. Therefore, a broad discussion is needed about the possible disease effects in pregnancy, as well as the risks of fetal exposure to the medications used, especially biological therapy. This study aimed to describe the management of 4 Crohn’s disease patients who received anti-TNF therapy during pregnancy and present a literature review. We reported 4 cases composed of young women who became pregnant while receiving anti-TNF agents. The patients presented a satisfactory response to the clinical treatment and the pregnancies progressed without complications. We did not observe maternal or embryonic toxicity, or unfavorable outcomes. The available data point to inflammatory activity as the main risk factor for unfavorable gestational evolution to date, and showed anti-TNF therapy to be safe during pregnancy and breastfeeding. However, the benefits and risks must be discussed with the patient and management decisions should be taken on an individual basis.
    Type of Medium: Online Resource
    ISSN: 1662-0631
    URL: Article
    DOI: 10.1159/000493921
    Language: English
    Publisher: S. Karger AG
    Publication Date: 2018
    detail.hit.zdb_id: 2440540-1
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  • 6
    Online Resource
    Online Resource
    Olmesartan-Induced Sprue Like Enteropathy
    S. Karger AG ; 2016
    In:  GE Portuguese Journal of Gastroenterology Vol. 23, No. 2 ( 2016-03), p. 101-105
    Details
    In: GE Portuguese Journal of Gastroenterology, S. Karger AG, Vol. 23, No. 2 ( 2016-03), p. 101-105
    Type of Medium: Online Resource
    ISSN: 2341-4545
    URL: Article
    DOI: 10.1016/j.jpge.2015.12.003
    Language: English
    Publisher: S. Karger AG
    Publication Date: 2016
    detail.hit.zdb_id: 2835774-7
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  • 7
    Online Resource
    Online Resource
    Immediate and Late Effects of Stress on Kidneys of Prepubertal and Adult Rats
    S. Karger AG ; 2018
    In:  Kidney and Blood Pressure Research Vol. 43, No. 6 ( 2018), p. 1919-1926
    Details
    In: Kidney and Blood Pressure Research, S. Karger AG, Vol. 43, No. 6 ( 2018), p. 1919-1926
    Abstract: 〈 b 〉 〈 i 〉 Background/Aims: 〈 /i 〉 〈 /b 〉 Stress had been related to physiological and morphological alterations in different organs, however kidney morphology and function after stress conditions are not well-known. To evaluate the effects of chronic stress on renal morphology in prepubertal and adult rats, and to investigate if the effects of induced stress remain stable or worsen following removal of the stress stimuli. 〈 b 〉 〈 i 〉 Methods: 〈 /i 〉 〈 /b 〉 Forty prepubertal rats (4 weeks old) and thirty six adult rats (10 weeks old) were used. Each age group was divided a into control group and a stressed group. Stressed animals were restrained in rigid tubes 2 hours per day for 6 weeks. Animals were euthanized 24 hours after the last stress stimulus for immediate evaluation (50% of the groups), and 6 weeks after the last stress stimulus for late evaluation (50% of the groups). Kidney weight and volume, cortical-medullar ratio, glomerular volumetric density, volume-weighted glomerular volume, and number of glomeruli per kidney were evaluated using the Image J software. 〈 b 〉 〈 i 〉 Results: 〈 /i 〉 〈 /b 〉 Prepubertal stressed animals showed reduced kidney weight and volume, and increased cortical-medullar ratio in comparison to its control group when evaluated immediately. Furthermore, stressed prepubertal and adult animals evaluated immediately had reduced glomerular volumetric density. Most importantly, all stressed groups exhibited reduced number of glomeruli per kidney. Other analyzed parameters did not difeer significantly the groups. 〈 b 〉 〈 i 〉 Conclusion: 〈 /i 〉 〈 /b 〉 Chronic stress induced before and after puberty led to irreversible glomerular loss, however, renal impairment was interrupted by removal of the stress stimuli.
    Type of Medium: Online Resource
    ISSN: 1420-4096 , 1423-0143
    URL: Article
    DOI: 10.1159/000496004
    Language: English
    Publisher: S. Karger AG
    Publication Date: 2018
    detail.hit.zdb_id: 1482922-8
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