In:
Cardiology, S. Karger AG, Vol. 142, No. 3 ( 2019), p. 149-157
Abstract:
〈 b 〉 〈 i 〉 Objectives: 〈 /i 〉 〈 /b 〉 To explore the association between single-nucleotide polymorphisms (SNPs) in 〈 i 〉 MTHFR 〈 /i 〉 and 〈 i 〉 APOE 〈 /i 〉 and the risk of CAD and, more importantly, the severity of CAD and the profile of serum lipids, we performed a case-control study in a Chinese Han population. 〈 b 〉 〈 i 〉 Methods: 〈 /i 〉 〈 /b 〉 A total of 1,207 cases of consecutive CAD-suspected inpatients were recruited, and 406 CAD cases and 231 non-CAD controls were enrolled for the final analysis after screening for exclusion criteria. All subjects had undergone coronary angiography, and the severity of CAD was evaluated by 2 cardiologists according to the Gensini scores. The genotypes of 〈 i 〉 MTHFR 〈 /i 〉 and 〈 i 〉 APOE 〈 /i 〉 were detected using real-time PCR, and then verified by Sanger sequencing. Environmental risk factors, such as age, sex, smoking, alcohol consumption, hypertension, diabetes, dyslipidemia, and BMI were collected. Statistical analyses (the χ 〈 sup 〉 2 〈 /sup 〉 test, binary logistic regression analysis, and ordinal polytomous logistic regression analysis) were performed with SPSS v16.0. 〈 b 〉 〈 i 〉 Results: 〈 /i 〉 〈 /b 〉 The genotypes of 〈 b 〉 〈 i 〉 〈 /i 〉 〈 /b 〉 all the subjects included in the CAD and non-CAD groups in this study were successfully detected, with an agreement of 100% with Sanger sequencing. The distributions of genotypes 〈 i 〉 CT 〈 /i 〉 and 〈 i 〉 TT 〈 /i 〉 at 〈 i 〉 MTHFR C667T 〈 /i 〉 were higher in CAD cases than in non-CAD controls (OR 1.99, 95% CI 1.34–2.95; OR 1.77, 95% CI 1.18–2.67; 〈 i 〉 p 〈 /i 〉 & #x3c; 0.05), whereas genotype 〈 i 〉 AC 〈 /i 〉 at 〈 i 〉 MTHFR A1298C 〈 /i 〉 was lower in CAD cases (OR 0.71, 95% CI 0.50–1.02; 〈 i 〉 p 〈 /i 〉 & #x3c; 0.05). A significant association was observed in genotypes 〈 i 〉 CT 〈 /i 〉 and 〈 i 〉 TT 〈 /i 〉 at 〈 i 〉 MTHFR C667T 〈 /i 〉 and the risk of CAD (OR 1.44, 95% CI 1.27–3.67; OR 1.56, 95% CI 0.88–2.78; 〈 i 〉 p 〈 /i 〉 & #x3c; 0.05). Both genotypes and alleles of 〈 i 〉 APOE 〈 /i 〉 were comparable in the CAD cases and non-CAD controls ( 〈 i 〉 p 〈 /i 〉 & #x3e; 0.05). The genotype 〈 i 〉 TT 〈 /i 〉 at 〈 i 〉 MTHFR C667T 〈 /i 〉 and ε4 〈 i 〉 + 〈 /i 〉 at 〈 i 〉 APOE 〈 /i 〉 were more likely to be found in the CAD subgroup with a Gensini score ≥72 ( 〈 i 〉 p 〈 /i 〉 = 0.040 and 〈 i 〉 p 〈 /i 〉 = 0.028, respectively). Meanwhile, in the patients with genotype 〈 i 〉 TT 〈 /i 〉 , 〈 i 〉 〈 /i 〉 a higher level of serum Hcy was detected, while genotype ε4+ patients possessed higher levels of serum apolipoprotein E (ApoE) and low-density lipoprotein cholesterol (LDL-C) than other genotypes. 〈 b 〉 〈 i 〉 Conclusion: 〈 /i 〉 〈 /b 〉 This study revealed that the SNP site of 〈 i 〉 MTHFR C667T 〈 /i 〉 is associated 〈 i 〉 〈 /i 〉 with the risk of CAD in this Chinese Han population. In addition, the genotypes of 〈 i 〉 TT 〈 /i 〉 in 〈 i 〉 MTHFR C667T 〈 /i 〉 and ε4 〈 i 〉 + 〈 /i 〉 in 〈 i 〉 APOE 〈 /i 〉 may increase the severity of CAD, and higher Hcy, LDL-C, and ApoE levels may be involved in this pathogenic process.
Type of Medium:
Online Resource
ISSN:
0008-6312
,
1421-9751
Language:
English
Publisher:
S. Karger AG
Publication Date:
2019
detail.hit.zdb_id:
1482041-9
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