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  • MDPI AG  (3)
  • 2015-2019  (3)
Materialart
Verlag/Herausgeber
  • MDPI AG  (3)
Sprache
Erscheinungszeitraum
  • 2015-2019  (3)
Jahr
  • 1
    In: Animals, MDPI AG, Vol. 8, No. 11 ( 2018-11-07), p. 200-
    Kurzfassung: Growth differentiation factor 9 (GDF9) plays an important role in the early folliculogenesis of sheep. This study investigated the mRNA expression of ovine GDF9 in different tissues by real-time PCR. GDF9 exhibits significantly higher levels of expression (p 〈 0.01) in the ovary, relative to other tissues, indicating that its expression is tissue specific. To explore the regulatory mechanism of this tissue-specific expression, the methylation level of one CpG island (−1453 to −1854) of GDF9 promoter in ovary and heart was determined. In this region (−1987 to −1750), only the mC-4 site was present in the Sp4 binding site showed differential methylation between the heart and ovary; with increased (p 〈 0.01) methylation being observed in the heart. Additionally, the methylation level was negatively correlated with GDF9 mRNA expression (R = −0.75, p = 0.012), indicating that the methylation of this site plays an important role in transcriptional regulation of GDF9. The methylation effect of the mC-4 site was confirmed by using dual-luciferase. Site-directed mutation (methylation) of mC-4 site significantly reduced (p 〈 0.05) basal transcriptional activity of GDF9 promoter in oocytes. These results imply that methylation of GDF9 promoter CpG island mC-4 site may affect the binding of the Sp4 transcription factor to the GDF9 promoter region in sheep, thereby regulating GDF9 expression and resulting in a tissue-specific expression.
    Materialart: Online-Ressource
    ISSN: 2076-2615
    Sprache: Englisch
    Verlag: MDPI AG
    Publikationsdatum: 2018
    ZDB Id: 2606558-7
    SSG: 23
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 2
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 20, No. 6 ( 2019-03-26), p. 1519-
    Kurzfassung: Cadmium is a common environmental pollutant that causes bone damage. However, the effects of cadmium on the osteogenic differentiation of bone marrow mesenchymal stem cells (BMMSCs) and its mechanism of action in this process are unclear. Here, we determined the effects of cadmium chloride (CdCl2) on the osteogenic differentiation of BMMSCs and the potential mechanism involved in this process. As determined in the present investigation, CdCl2, in a concentration-dependent manner, affected the viability of BMMSCs and their cytoskeletons. Exposure to 0.1 or 0.2 µM CdCl2 inhibited osteogenic differentiation of BMMSCs, which was reflected in the down-regulation of osteoblast-related genes (ALP, OCN, Runx2, OSX, and OPN); in suppression of the protein expression of alkaline phosphatase (ALP) and runt-related transcription factor 2 (Runx2); and in decreased ALP activity and capacity for mineralization. Moreover, mRNA microarray was performed to determine the roles of these factors in BMMSCs treated with CdCl2 in comparison to control BMMSCs. As determined with the microarrays, the Wingless-type (Wnt), mothers against decapentaplegic and the C. elegans gene Sam (SMAD), and Janus kinase-Signal Transducers and Activators of Transcription (JAK-STAT) signaling pathways were involved in the effects caused by CdCl2. Moreover, during differentiation, the protein levels of Wnt3a, β-catenin, lymphoid enhancer factor 1 (LEF1), and T-cell factor 1 (TCF1) were reduced by CdCl2. The current research shows that CdCl2 suppresses the osteogenesis of BMMSCs via inhibiting the Wnt/β-catenin pathway. The results establish a previously unknown mechanism for bone injury induced by CdCl2.
    Materialart: Online-Ressource
    ISSN: 1422-0067
    Sprache: Englisch
    Verlag: MDPI AG
    Publikationsdatum: 2019
    ZDB Id: 2019364-6
    SSG: 12
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 3
    In: Energies, MDPI AG, Vol. 12, No. 20 ( 2019-10-16), p. 3932-
    Kurzfassung: Shale oil is an unconventional kind of oil and gas resource with great potential. China has huge reserves of shale oil, and shale oil resources are abundant in the third submember of the Triassic Chang 7 member in the southern Ordos Basin. At present, this area is in the initial stage of shale oil exploration and development. The reservoir pore is one of the key factors affecting oil accumulation, drilling safety, and oil production. It is also an important reservoir parameter that must be defined in the exploration stage. In general, the clay content in the shale section is high, and is prone to hydration. In order to study the effect of fluid on the pore type, structure, and distribution of shale oil reservoirs, experiments using X-ray diffraction, a porosity–permeability test, mercury porosimetry, rock casting thin section, and scanning electron microscopy were carried out. The experimental results show that the content of clay and quartz is very high in the studied formation. The pore porosity and permeability of the rock is highly heterogeneous because of the obvious stratigraphic bedding and interbeds. Microstructural observation of rocks shows that the main pore types are intergranular pores, intragranular pores, intercrystalline pores, and organic pores. Crack types are dissolution cracks, contraction cracks of organic matter, and abnormal pressure structural cracks. After hydration, the porosity of rock will increase in varying degrees, and pore size, pore content in different sizes, and pore structure will also change. The results show that the pores of tuff mainly changes at the initial stage of hydration, and the pore change of tuff is the most obvious within 6 hours of soaking in clear water. The influence of hydration on the pore of shale is greater than that of tuff, but the main change stage is later than tuff, and the pore change of shale is the most obvious within 12 to 24 hours of soaking in clear water. The soaking experiment of water-based drilling fluid (WBM-SL) shows that it can plug a certain size of holes and cracks and form a protective layer on the rock surface, thus effectively reducing hydration. In actual construction, multisized solid particles should be allocated in drilling fluid according to the formation pore’s characteristics, and the stability of the protective layer should be guaranteed. This can reduce the accident of well leakage and collapse and is conducive to the efficient and safe development of shale oil.
    Materialart: Online-Ressource
    ISSN: 1996-1073
    Sprache: Englisch
    Verlag: MDPI AG
    Publikationsdatum: 2019
    ZDB Id: 2437446-5
    Standort Signatur Einschränkungen Verfügbarkeit
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