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  • Chemistry  (3)
  • Alkalinity, total; Aragonite saturation state; Benthos; Bicarbonate ion; BIOACID; Biological Impacts of Ocean Acidification; Bottles or small containers/Aquaria (〈20 L); Calcite saturation state; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Chlorophyta; Chromista; CO2 vent; Coast and continental shelf; Distance; Field experiment; Figure; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gross photosynthesis rate, oxygen; Identification; Location; Macroalgae; OA-ICC; Ocean Acidification International Coordination Centre; Ochrophyta; Oxygen; Oxygen, standard error; Padina australis; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH; Potentiometric; Primary production/Photosynthesis; Registration number of species; Replicate; Run Date/Time; Salinity; Single species; Site; South Pacific; Species; Temperate; Temperature, water; Treatment; Type; Uniform resource locator/link to reference  (1)
  • 2015-2019  (1)
  • 1995-1999  (1)
  • 1990-1994  (2)
Document type
Keywords
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  • 2015-2019  (1)
  • 1995-1999  (1)
  • 1990-1994  (2)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Effects of biofilm structures on oxygen distribution and mass transport (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 43 (1994), S. 1131-1138 
    Details
    ISSN: 0006-3592
    Keywords: confocal microscopy ; microelectrodes ; cell clusters ; pores ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Aerobic biofilms were found to have a complex structure consisting of microbial cell clusters (discrete aggregates of densely packed cells) and interstitial voids. The oxygen distribution was strongly correlated with these strutures. The voids facilitated oxygen transport from the bulk liquid through the biofilm, supplying approximately 50% of the total oxygen consumed by the cells. The mass transport rate from the bulk liquid is influenced by the biofilm structure; the observed exchange surface of the biofilm is twice that calculated for a simple planar geometry. The oxygen diffusion occurred in the direction normal to the cluster surfaces, the horizontal and vertical components of the oxygen gradients were of equal importance. Consequently, for calculations of mass transfer rates a three-dimensional model is necessary. These findings imply that to accurately describe biofilm activity, the relation between the arrangement of structural components and mass transfer must be undrstood. © 1994 John Wiley & Sons, Inc.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260431118
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Liquid flow in heterogeneous biofilms (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 44 (1994), S. 636-641 
    Details
    ISSN: 0006-3592
    Keywords: biofilm ; hydrodynamics ; mass transport ; particle tracking ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Liquid flow was studied in aerobic biofilms, consisting of microbial cell clusters (discrete aggregates of densely packed cells) and interstitial voids. Fluorescein microinjection was used as a qualitative technique to determine the presence of flow in cell clusters and voids. Flow velocity profiles were determined by tracking fluorescent latex spheres using confocal microscopy. Liquid was flowing through the voids and was stagnant in the cell clusters. Consequently, in voids both diffusion and convection may contribute to mass transfer, whereas in cell clusters diffusion is the dominant factor. The flow velocity in the biofilm depended on the average flow velocity of the bulk liquid. The velocity profiles in biofilms were linear and the velocity was zero at the substratum surface. The velocity gradients within biofilms were 50% of that near walls without biofilm coverage. The influence of the biofilm roughness on the flow velocity profiles was similar to that caused by rigid roughness elements. © 1994 John Wiley & Sons, Inc.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260440510
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Measurement of local diffusion coefficients in biofilms by microinjection and confocal microscopy (1997)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 53 (1997), S. 151-158 
    Details
    ISSN: 0006-3592
    Keywords: biofilms ; biofilm structure ; diffusivity ; mass transport in biofilms ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A new technique for the determination of local diffusion coefficients in biofilms is described. It is based on the microinjection of fluorescent dyes and quantitative analysis of the subsequent plume formation using confocal laser microscopy. The diffusion coefficients of fluorescein (MW 332), TRITC-IgG (MW 150000) and phycoerythrin (MW 240000) were measured in the cell clusters and interstitial voids of a heterogeneous biofilm. The diffusivities measured in the voids were close to the theoretical values in water. Fluorescein had the same diffusivity in cell clusters, voids, and sterile medium. TRITC-IgG did not diffuse in cell clusters, presumably due to binding to the cell cluster matrix. After treatment of the biofilm with bovine serum albumin, binding capacity decreased and the diffusion coefficient could be measured. The diffusivity of phycoerythrin in cell clusters was impeded by 41%, compared to interstitial voids. From the diffusion data of phycoerythrin it was further calculated that the cell cluster matrix had the characteristics of a gel with 0.6 nm thick fibers and pore diameters of 80 nm. © 1997 John Wiley & Sons, Inc.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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    Paper (German National Licenses)
  • 4
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    Unknown
    Microsensor studies on Padina from a natural CO2 seep: implications of morphology on acclimation to low pH (2015)
    PANGAEA
    In:  Supplement to: Hofmann, Laurie C; Fink, Artur; Bischof, Kai; de Beer, Dirk (2015): Microsensor studies on Padina from a natural CO2 seep: implications of morphology on acclimation to low pH. Journal of Phycology, 51(6), 1106-1115, https://doi.org/10.1111/jpy.12347
    Details
    Publication Date: 2024-03-15
    Description: Low seawater pH can be harmful to many calcifying marine organisms, but the calcifying macroalgae Padina spp. flourish at natural submarine carbon dioxide seeps where seawater pH is low. We show that the microenvironment created by the rolled thallus margin of Padina australis facilitates supersaturation of CaCO3 and calcifi-cation via photosynthesis-induced elevated pH. Using microsensors to investigate oxygen and pH dynamics in the microenvironment of P. australis at a shallow CO2 seep, we found that, under saturating light, the pH inside the microenvironment (pHME) was higher than the external seawater (pHSW) at all pHSW levels investigated, and the difference (i.e., pHME-pHSW) increased with decreasing pHSW (0.9 units at pHSW 7.0). Gross photosynthesis (Pg) inside the microenvironment increased with decreasing pHSW, but algae from the control site reached a threshold at pH 6.5. Seep algae showed no pH threshold with respect to Pg within the pHSW range investigated. The external carbonic anhydrase (CA) inhibitor, acetazolamide, strongly inhibited Pg of P. australis at pHSW 8.2, but the effect was diminished under low pHSW (6.4-7.5), suggesting a greater dependence on membrane-bound CA for the dehydration of HCO3- ions during dissolved inorganic carbon uptake at the higher pHSW. In comparison, a calcifying green alga, Halimeda cuneata f. digitata, was not inhibited by AZ, suggesting efficient bicarbonate transport. The ability of P. australis to elevate pHME at the site of calcification and its strong dependence on CA may explain why it can thrive at low pHSW.
    Keywords: Alkalinity, total; Aragonite saturation state; Benthos; Bicarbonate ion; BIOACID; Biological Impacts of Ocean Acidification; Bottles or small containers/Aquaria (〈20 L); Calcite saturation state; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Chlorophyta; Chromista; CO2 vent; Coast and continental shelf; Distance; Field experiment; Figure; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gross photosynthesis rate, oxygen; Identification; Location; Macroalgae; OA-ICC; Ocean Acidification International Coordination Centre; Ochrophyta; Oxygen; Oxygen, standard error; Padina australis; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH; Potentiometric; Primary production/Photosynthesis; Registration number of species; Replicate; Run Date/Time; Salinity; Single species; Site; South Pacific; Species; Temperate; Temperature, water; Treatment; Type; Uniform resource locator/link to reference
    Type: Dataset
    Format: text/tab-separated-values, 40403 data points
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    DATA PANGAEA
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