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  • Springer  (5)
  • Nature Publishing Group (NPG)  (3)
  • Berlin, Heidelberg :Springer Berlin / Heidelberg,  (1)
  • 2015-2019  (4)
  • 2000-2004  (1)
  • 1985-1989  (4)
  • 1
    Online Resource
    Online Resource
    Berlin, Heidelberg :Springer Berlin / Heidelberg,
    Keywords: Floodplains-Europe, Central. ; Riparian ecology-Europe, Central. ; Electronic books.
    Type of Medium: Online Resource
    Pages: 1 online resource (166 pages)
    Edition: 1st ed.
    ISBN: 9783662554555
    DDC: 570
    Language: German
    Note: Intro -- Vorwort -- Inhaltsverzeichnis -- 1 Grundwasserlebensräume in Auenwäldern -- 2 Quellen in Auenlandschaften -- 3 Geomorphologie von Flussläufen -- 4 Böden in Auenwäldern -- 5 Flussbegleitende Moore und Sümpfe -- 6 Hydromorphologie in Auenbiotopen -- 7 Paläoökologie der Auenbiotope -- 8 Pflanzengesellschaften in Auenbiotopen -- 9 Entomofauna in Auenbiotopen -- 10 Auentypische Mollusken und Krebstiere -- 11 Ichthyofauna der Auengewässer -- 12 Herpetofauna in Auen -- 13 Auentypische Avifauna -- 14 Säugetierfauna in Auenwäldern -- 15 Auenrevitalisierung -- Serviceteil -- Glossar auenökologischer Fachbegriffe -- Stichwortverzeichnis.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Journal of computer aided molecular design 14 (2000), S. 403-409 
    ISSN: 1573-4951
    Keywords: cyclin-dependent kinase ; CDK1 ; inhibitor ; olomoucine ; structure-based design
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Cyclin-dependent kinase 1 (CDK1), an enzyme participating in the regulation of the cell cycle, constitutes a possible target in the search for new antitumor agents. Starting from the purine derivative olomoucine and following a structure-based approach, potent inhibitors of this enzyme were rapidly identified. The molecular modeling aspects of this work are described.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 205 (1986), S. 494-500 
    ISSN: 1617-4623
    Keywords: Signal sequence ; Gene regulation ; Export ; Codon usage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The nucleotide sequence of the ompV gene of Vibrio cholerae was determined. The product of the gene is a 28,000 dalton protein which, after the removal of a 19 amino acid signal sequence, produces a mature outer membrane protein of 26,000 daltons. The cleavage site was determined by amino-terminal amino acid sequencing of the purified mature protein. The DNA upstream of the gene shows the presence of a typical promoter region as judged from the Escherichia coli consensus information; however, the Shine-Dalgarno sequence is associated with a region capable of forming a secondary structure in the mRNA. The formation of this structure would inhibit binding of the mRNA to the ribosome and reduce translation. It is proposed that this structure is recognized by a positive activator in V. cholerae and because of its absence in E. coli ompV is poorly expressed. The distribution of rare codons within ompV suggests that they may serve to slow down the translation of particular domains such that the nascent polypeptide has an opportunity to take up its conformation without interference from the later formed regions. Such a mechanism could aid localization of the protein if export were by a cotranslational secretion system.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 205 (1986), S. 501-506 
    ISSN: 1617-4623
    Keywords: Signal sequence ; Antigenic epitopes ; Outer membrane protein ; Immunogenicity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Fusion proteins comprising the amino-terminal 99 amino acids of the bacteriophage MS2 replicase and various portions of OmpV a major outer membrane protein of Vibrio cholerae were expressed in Escherichia coli K12. These fusions were expressed under the control of the PL promoter of bacteriophage λ, and expression was controlled using a cIts repressor. Fusions occurring within the secretory signal sequence of OmpV gave rise to the production of mature OmpV. The efficiency, however, decreased with progressive deletion of the signal sequence within the fusions. The reactivity of various OmpV fusions with antisera raised against purified OmpV and whole bacteria demonstrated the existence of two antigenic domains: one present in the denatured form and another in the membrane-associated form of OmpV. These domains correspond to markedly hydrophilic regions of the protein as would be predicted for surface-exposed epitopes.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Antonie van Leeuwenhoek 53 (1987), S. 431-434 
    ISSN: 1572-9699
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The genome of Neisseria gonorrhoeae harbours many gene loci for the production of variant pili. Strain MS11 has two expression genes (pilE) with promoter and complete coding sequences. The remaining genes are silent (pilS) lacking the promoter and the conservative amino terminals coding sequences of pilin. The pilus genes consist of six variable minicassettes (mc's), that are flancked by strictly conserved sequences. Upon phase (P+ to P+) and antigenic (P+ to P−, or vice versa) transitions minicassettes from silent loci are transferred from silent pilus gene copies to the expression gene by gene conversion. P− variants resulting from such rearrangements still produce pilin mRNA as well as pilin, but only a few are found on the surface of those gonococci.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1572-9699
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 7
    Publication Date: 2018-11-27
    Description: Escherichia coli “Marionette” strains with 12 highly optimized small-molecule sensors 〈i〉Escherichia〈/i〉 coli “Marionette” strains with 12 highly optimized small-molecule sensors, Published online: 26 November 2018; doi:10.1038/s41589-018-0168-3 A directed evolution approach was applied to optimize a set of 12 small-molecule-responsive biosensors, which led to the engineering of “Marionette” strains of Escherichia coli incorporating these sensors for biotechnological applications.
    Print ISSN: 1552-4450
    Electronic ISSN: 1552-4469
    Topics: Biology , Chemistry and Pharmacology
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  • 8
    Publication Date: 2015-02-04
    Description: Nature Geoscience 8, 122 (2015). doi:10.1038/ngeo2349 Authors: Hanno Meyer, Thomas Opel, Thomas Laepple, Alexander Yu Dereviagin, Kirstin Hoffmann & Martin Werner Relative to the past 2,000 years, the Arctic region has warmed significantly over the past few decades. However, the evolution of Arctic temperatures during the rest of the Holocene is less clear. Proxy reconstructions, suggest a long-term cooling trend throughout the mid- to late Holocene, whereas climate model simulations show only minor changes or even warming. Here we present a record of the oxygen isotope composition of permafrost ice wedges from the Lena River Delta in the Siberian Arctic. The isotope values, which reflect winter season temperatures, became progressively more enriched over the past 7,000 years, reaching unprecedented levels in the past five decades. This warming trend during the mid- to late Holocene is in opposition to the cooling seen in other proxy records. However, most of these existing proxy records are biased towards summer temperatures. We argue that the opposing trends are related to the seasonally different orbital forcing over this interval. Furthermore, our reconstructed trend as well as the recent maximum are consistent with the greenhouse gas forcing and climate model simulations, thus reconciling differing estimates of Arctic and northern high-latitude temperature evolution during the Holocene.
    Print ISSN: 1752-0894
    Electronic ISSN: 1752-0908
    Topics: Geosciences
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  • 9
    Publication Date: 2016-05-29
    Description: Article Protein aggregates are associated with a wide variety of diseases. Here, in order to address how protein aggregation affects cellular homoeostasis, the authors describe a method to rapidly create protein aggregates in living cells and organisms with precise spatial and temporal control. Nature Communications doi: 10.1038/ncomms11689 Authors: Yusuke Miyazaki, Kota Mizumoto, Gautam Dey, Takamasa Kudo, John Perrino, Ling-chun Chen, Tobias Meyer, Thomas J. Wandless
    Electronic ISSN: 2041-1723
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General , Physics
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