In:
Frontiers in Plant Science, Frontiers Media SA, Vol. 12 ( 2021-6-8)
Abstract:
The use of plant biostimulants contributes to more sustainable and environmentally friendly farming techniques and offers a sustainable alternative to mitigate the adverse effects of stress. Protein hydrolysate-based biostimulants have been described to promote plant growth and reduce the negative effect of abiotic stresses in different crops. However, limited information is available about their mechanism of action, how plants perceive their application, and which metabolic pathways are activating. Here we used a multi-trait high-throughput screening approach based on simple RGB imaging and combined with untargeted metabolomics to screen and unravel the mode of action/mechanism of protein hydrolysates in Arabidopsis plants grown in optimal and in salt-stress conditions (0, 75, or 150 mM NaCl). Eleven protein hydrolysates from different protein sources were used as priming agents in Arabidopsis seeds in three different concentrations (0.001, 0.01, or 0.1 μl ml –1 ). Growth and development-related traits as early seedling establishment, growth response under stress and photosynthetic performance of the plants were dynamically scored throughout and at the end of the growth period. To effectively classify the functional properties of the 11 products a Plant Biostimulant Characterization (PBC) index was used, which helped to characterize the activity of a protein hydrolysate based on its ability to promote plant growth and mitigate stress, and to categorize the products as plant growth promoters, growth inhibitors and/or stress alleviator. Out of 11 products, two were identified as highly effective growth regulators and stress alleviators because they showed a PBC index always above 0.51. Using the untargeted metabolomics approach, we showed that plants primed with these best performing biostimulants had reduced contents of stress-related molecules (such as flavonoids and terpenoids, and some degradation/conjugation compounds of phytohormones such as cytokinins, auxins, gibberellins, etc.), which alleviated the salt stress response-related growth inhibition.
Type of Medium:
Online Resource
ISSN:
1664-462X
DOI:
10.3389/fpls.2021.626301
DOI:
10.3389/fpls.2021.626301.s001
DOI:
10.3389/fpls.2021.626301.s002
DOI:
10.3389/fpls.2021.626301.s003
DOI:
10.3389/fpls.2021.626301.s004
DOI:
10.3389/fpls.2021.626301.s005
DOI:
10.3389/fpls.2021.626301.s006
DOI:
10.3389/fpls.2021.626301.s007
DOI:
10.3389/fpls.2021.626301.s008
DOI:
10.3389/fpls.2021.626301.s009
DOI:
10.3389/fpls.2021.626301.s010
DOI:
10.3389/fpls.2021.626301.s011
DOI:
10.3389/fpls.2021.626301.s012
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2021
detail.hit.zdb_id:
2687947-5
detail.hit.zdb_id:
2613694-6
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