In:
PLOS ONE, Public Library of Science (PLoS), Vol. 18, No. 11 ( 2023-11-6), p. e0294031-
Abstract:
Riemerella (R . ) anatipestifer poses a significant threat to ducks, resulting in mortality rates ranging from 5–75%. This disease is highly infectious and economically consequential for domestic ducks. Although other avian species, such as chickens, also display susceptibility, the impact is comparatively less severe than in ducks. IL-17A has a pronounced correlation with R . anatipestifer infection in ducks, which is less in chickens. This study performed an in vitro transcriptome analysis using chicken splenic lymphocytes collected at 4-, 8-, and 24-hour intervals following R . anatipestifer stimulation. The primary objective was to discern the differentially expressed genes, with a specific focus on IL-17A and IL-17F expression. Moreover, an association between specific miRNAs with NOS2 and CCL5 was identified. The manifestation of riemerellosis in chickens was linked to heightened expression of Th1- and Th2-associated cells, while Th17 cells exhibited minimal involvement. This study elucidated the mechanism behind the absence of a Th17 immune response, shedding light on its role throughout disease progression. Additionally, through small RNA sequencing, we identified a connection between miRNAs, specifically miR-456-3p and miR-16-5p, and their respective target genes NOS2 and CCL5. These miRNAs are potential regulators of the inflammatory process during riemerellosis in chickens.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0294031
DOI:
10.1371/journal.pone.0294031.g001
DOI:
10.1371/journal.pone.0294031.g002
DOI:
10.1371/journal.pone.0294031.g003
DOI:
10.1371/journal.pone.0294031.g004
DOI:
10.1371/journal.pone.0294031.g005
DOI:
10.1371/journal.pone.0294031.g006
DOI:
10.1371/journal.pone.0294031.g007
DOI:
10.1371/journal.pone.0294031.g008
DOI:
10.1371/journal.pone.0294031.t001
DOI:
10.1371/journal.pone.0294031.t002
DOI:
10.1371/journal.pone.0294031.t003
DOI:
10.1371/journal.pone.0294031.t004
DOI:
10.1371/journal.pone.0294031.t005
DOI:
10.1371/journal.pone.0294031.s001
DOI:
10.1371/journal.pone.0294031.s002
DOI:
10.1371/journal.pone.0294031.s003
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2267670-3
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