In:
Frontiers in Cell and Developmental Biology, Frontiers Media SA, Vol. 9 ( 2021-9-28)
Abstract:
Gallstone disease is a worldwide common disease. However, the knowledge concerning the gallbladder in the pathogenesis of cholesterol gallstone formation remains limited. In this study, using single-cell RNA sequencing (scRNA-seq) to obtain the transcriptome of gallbladder cells, we showed cellular heterogeneity and transcriptomic dynamics in murine gallbladder cells during the process of lithogenesis. Our results indicated gallbladder walls were subjected to remodeling during the process of lithogenesis. The major molecular events that happened included proliferation of epithelial cells, infiltration of immune-cells, activation of angiogenesis, and extracellular matrix modulation. Furthermore, we observed partial reversal of gallbladder cell transcriptomes by ursodeoxycholic acid treatment. This work thus provides novel and integral knowledges on the cellular changes during lithogenesis, which is of great significance to the understanding of pathogenesis and treatment of cholesterol gallstone.
Type of Medium:
Online Resource
ISSN:
2296-634X
DOI:
10.3389/fcell.2021.714271
DOI:
10.3389/fcell.2021.714271.s001
DOI:
10.3389/fcell.2021.714271.s002
DOI:
10.3389/fcell.2021.714271.s003
DOI:
10.3389/fcell.2021.714271.s004
DOI:
10.3389/fcell.2021.714271.s005
DOI:
10.3389/fcell.2021.714271.s006
DOI:
10.3389/fcell.2021.714271.s007
DOI:
10.3389/fcell.2021.714271.s008
DOI:
10.3389/fcell.2021.714271.s009
DOI:
10.3389/fcell.2021.714271.s010
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2021
detail.hit.zdb_id:
2737824-X
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