In:
LEGUME RESEARCH - AN INTERNATIONAL JOURNAL, Agricultural Research Communication Center, , No. OF ( 2020-07-28)
Abstract:
Background: Canine distemper virus (CDV) is one of the important causes for canine gastroenteritis. Its genome codes for six structural proteins N, P, M, F, H and L, of which H is important for viral pathogenesis. Availability of CDV molecular epidemiological data is sparse. Our study reports for the first time, isolation of enteric CDV from India and its molecular epidemiology. Methods: We isolated the circulating wild type CDV from cases of canine gastroenteritis recorded during 2019 in India. Partial H gene of the isolates was amplified and phylogeny was reconstructed with other isolates from the NCBI database using MUSCLE from MEGA7. Results: The multiple sequence alignment of the partial H gene of circulating CDV with reference isolates revealed 88-95% nucleotide identity, and 83-91% amino acid identity along with the presence of Nde1 restriction site at position 1571-1576 that is typical of wild type CDV isolates. Interestingly, phylogenetic analysis revealed that the circulating CDV were grouped with three different lineages i.e., South America-2, Asia-4, and Asia-5/India-1 and distantly related with the vaccine strains. Our study strengthens the need for the development of a relevant vaccine comprising of circulating viral strains for effective vaccination strategies.
Type of Medium:
Online Resource
ISSN:
0976-0571
,
0250-5371
DOI:
10.18805/ijar.B-3989
Language:
Unknown
Publisher:
Agricultural Research Communication Center
Publication Date:
2020
detail.hit.zdb_id:
407856-1
Permalink