In:
PLOS ONE, Public Library of Science (PLoS), Vol. 18, No. 8 ( 2023-8-11), p. e0288782-
Abstract:
The aim of this study is to evaluate if single nucleotide polymorphisms (SNPs) in WNT6 and WNT10A are associated with the risk of dental pulp calcification in orthodontic patients. This cross-sectional study followed the “Strengthening the Reporting of Genetic Association Studies” (STREGA) guidelines. Panoramic radiographs (pre- and post-orthodontic treatment) and genomic DNA from 132 orthodontic patients were studied. Dental pulp calcification (pulp stones and/or pulp space narrowing) was recorded in upper and lower first molars. The SNPs in WNT6 and WNT10A ( rs7349332 , rs3806557 , rs10177996 , and rs6754599 ) were assessed through genotyping analysis using DNA extracted from buccal epithelial cells. The association between pulp calcification and SNPs were analyzed using allelic and genotypic distributions and haplotype frequencies (p 〈 0.05). Prevalence of dental pulp calcification was 42.4% in the 490 studied molars. In the genotypic analysis, the SNPs in WNT10A showed a statistically significant value for molar calcification (p = 0.027 for rs1017799 ), upper molar calcification (p = 0.040 for rs1017799 ) (recessive model), and molar calcification (p = 0.046 for rs3806557 ) (recessive model). In the allelic distribution, the allele C of the SNP rs10177996 in WNT10A was associated with molar calcifications (p = 0.042) and with upper first molar calcification (p = 0.035). Nine combinations of haplotypes showed statistically significant value (p 〈 0.05). The findings of this study indicates that SNPs in WNT10A and WNT6 are associated with dental pulp calcification in molars after orthodontic treatment and may be considered as biomarkers for dental pulp calcification.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0288782
DOI:
10.1371/journal.pone.0288782.g001
DOI:
10.1371/journal.pone.0288782.t001
DOI:
10.1371/journal.pone.0288782.t002
DOI:
10.1371/journal.pone.0288782.t003
DOI:
10.1371/journal.pone.0288782.t004
DOI:
10.1371/journal.pone.0288782.t005
DOI:
10.1371/journal.pone.0288782.t006
DOI:
10.1371/journal.pone.0288782.s001
DOI:
10.1371/journal.pone.0288782.s002
DOI:
10.1371/journal.pone.0288782.s003
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2267670-3
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