In:
PLOS Neglected Tropical Diseases, Public Library of Science (PLoS), Vol. 15, No. 9 ( 2021-9-7), p. e0009760-
Kurzfassung:
In insects the reserve proteins are stored in the oocytes into endocytic-originated vesicles named yolk organelles. VPS38/UVRAG and ATG14 are the variant regulatory subunits of two class-III ATG6/Beclin1 PI3K complexes that regulate the recruitment of the endocytic (complex II) and autophagic (complex I) machineries. In a previous work from our group, we found that the silencing of ATG6/Beclin1 resulted in the formation of yolk-deficient oocytes due to defects in the endocytosis of the yolk proteins. Because ATG6/Beclin1 is present in the two above-described PI3K complexes, we could not identify the contributions of each complex to the yolk defective phenotypes. To address this, here we investigated the role of the variant subunits VPS38/UVRAG (complex II, endocytosis) and ATG14 (complex I, autophagy) in the biogenesis of the yolk organelles in the insect vector of Chagas Disease Rhodnius prolixus . Interestingly, the silencing of both genes phenocopied the silencing of ATG6/Beclin1, generating 1) accumulation of yolk proteins in the hemolymph; 2) white, smaller, and yolk-deficient oocytes; 3) abnormal yolk organelles in the oocyte cortex; and 4) unviable F1 embryos. However, we found that the similar phenotypes were the result of a specific cross-silencing effect among the PI3K subunits where the silencing of VPS38/UVRAG and ATG6/Beclin1 resulted in the specific silencing of each other, whereas the silencing of ATG14 triggered the silencing of all three PI3K components. Because the silencing of VPS38/UVRAG and ATG6/Beclin1 reproduced the yolk-deficiency phenotypes without the cross silencing of ATG14, we concluded that the VPS38/UVRAG PI3K complex II was the major contributor to the previously observed phenotypes in silenced insects. Altogether, we found that class-III ATG6/Beclin1 PI3K complex II (VPS38/UVRAG) is essential for the yolk endocytosis and that the subunits of both complexes are under an unknown transcriptional regulatory system.
Materialart:
Online-Ressource
ISSN:
1935-2735
DOI:
10.1371/journal.pntd.0009760
DOI:
10.1371/journal.pntd.0009760.g001
DOI:
10.1371/journal.pntd.0009760.g002
DOI:
10.1371/journal.pntd.0009760.g003
DOI:
10.1371/journal.pntd.0009760.g004
DOI:
10.1371/journal.pntd.0009760.g005
DOI:
10.1371/journal.pntd.0009760.g006
DOI:
10.1371/journal.pntd.0009760.s001
DOI:
10.1371/journal.pntd.0009760.s002
DOI:
10.1371/journal.pntd.0009760.s003
DOI:
10.1371/journal.pntd.0009760.s004
DOI:
10.1371/journal.pntd.0009760.s005
DOI:
10.1371/journal.pntd.0009760.s006
DOI:
10.1371/journal.pntd.0009760.s007
DOI:
10.1371/journal.pntd.0009760.s008
DOI:
10.1371/journal.pntd.0009760.r001
DOI:
10.1371/journal.pntd.0009760.r002
DOI:
10.1371/journal.pntd.0009760.r003
DOI:
10.1371/journal.pntd.0009760.r004
Sprache:
Englisch
Verlag:
Public Library of Science (PLoS)
Publikationsdatum:
2021
ZDB Id:
2429704-5
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