In:
International Archives of Allergy and Immunology, S. Karger AG, Vol. 146, No. Suppl. 1 ( 2008), p. 2-6
Abstract:
〈 i 〉 Background: 〈 /i 〉 Conserved noncoding sequence-1 (CNS-1) is an important regulatory element for T helper 2 cytokine expression. IL-4, IL-5 and IL-13 expression as well as serum IgE level were attenuated in CNS-1 〈 sup 〉 –/– 〈 /sup 〉 mice. 〈 i 〉 Method: 〈 /i 〉 CNS-1 〈 sup 〉 –/– 〈 /sup 〉 and CNS-1 〈 sup 〉 +/+ 〈 /sup 〉 mice were sensitized with ovalbumin (OVA) followed by antigen challenge. The number of eosinophils and T helper 2 cytokine concentration in the bronchoalveolar lavage fluid, OVA-specific IgE antibody (Ab) in the serum and bronchial responsiveness to methacholine were examined. 〈 i 〉 Results: 〈 /i 〉 Bronchoalveolar lavage fluid eosinophilia was significantly attenuated in CNS-1 〈 sup 〉 –/– 〈 /sup 〉 mice compared to CNS-1 〈 sup 〉 +/+ 〈 /sup 〉 mice, which were sensitized with OVA/aluminum once. OVA-specific IgE Ab was also attenuated. When mice were sensitized with OVA/aluminum twice, induction of eosinophilia and OVA-specific IgE Ab was not significantly different between CNS-1 〈 sup 〉 –/– 〈 /sup 〉 and CNS-1 〈 sup 〉 +/+ 〈 /sup 〉 mice. 〈 i 〉 Conclusion: 〈 /i 〉 CNS-1 locus regulates eosinophilic inflammation in vivo.
Type of Medium:
Online Resource
ISSN:
1018-2438
,
1423-0097
Language:
English
Publisher:
S. Karger AG
Publication Date:
2008
detail.hit.zdb_id:
1482722-0
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