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  • Wiley  (69)
  • 2020-2024  (69)
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Verlag/Herausgeber
  • Wiley  (69)
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  • 2020-2024  (69)
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FID
Fachgebiete(RVK)
  • 1
    Online-Ressource
    Online-Ressource
    Numerical investigation on the spewing mechanism of earth pressure balance shield in a high‐pressure water‐rich sand stratum
    Wiley ; 2023
    In:  Deep Underground Science and Engineering Vol. 2, No. 1 ( 2023-03), p. 74-87
    Details
    In: Deep Underground Science and Engineering, Wiley, Vol. 2, No. 1 ( 2023-03), p. 74-87
    Kurzfassung: Mechanism of the earth pressure balance (EPB) screw conveyor spewing in tunneling within the high‐pressure water‐rich sand layer was analyzed. A simple theoretical model and a two‐dimensional multiphysical coupling model were developed for the EPB spewing. The critical permeability coefficient is obtained for the EPB screw conveyor spewing.
    Materialart: Online-Ressource
    ISSN: 2097-0668 , 2770-1328
    URL: Issue
    URL: Article
    DOI: 10.1002/dug2.v2.1
    DOI: 10.1002/dug2.12032
    Sprache: Englisch
    Verlag: Wiley
    Publikationsdatum: 2023
    Standort Signatur Einschränkungen Verfügbarkeit
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    Online-Ressource
  • 2
    Online-Ressource
    Online-Ressource
    Framework‐Hotspot Enhanced Trans Cleavage of CRISPR‐Cas12a for Clinical Samples Detection
    Wiley ; 2023
    In:  Angewandte Chemie Vol. 135, No. 32 ( 2023-08-07)
    Details
    In: Angewandte Chemie, Wiley, Vol. 135, No. 32 ( 2023-08-07)
    Kurzfassung: The trans‐cleavage property of CRISPR‐Cas12a system makes it an excellent tool for disease diagnosis. Nevertheless, most methods based on CRISPR‐Cas system still require pre‐amplification of the target to achieve the desired detection sensitivity. Here we generate Framework‐Hotspot reporters (FHRs) with different local densities to investigate their effect on trans‐cleavage activity of Cas12a. We find that the cleavage efficiency increases and the cleavage rate accelerates with increasing reporter density. We further construct a modular sensing platform with CRISPR‐Cas12a‐based target recognition and FHR‐based signal transduction. Encouragingly, this modular platform enables sensitive (100 fM) and rapid ( 〈 15 min) detection of pathogen nucleic acids without pre‐amplification, as well as detection of tumor protein markers in clinical samples. The design provides a facile strategy for enhanced trans cleavage of Cas12a, which accelerates and broadens its applications in biosensing.
    Materialart: Online-Ressource
    ISSN: 0044-8249 , 1521-3757
    URL: Issue
    URL: Article
    DOI: 10.1002/ange.v135.32
    DOI: 10.1002/ange.202305536
    RVK:
    VA 2100
    RVK:
    VN 5020
    Sprache: Englisch
    Verlag: Wiley
    Publikationsdatum: 2023
    ZDB Id: 505868-5
    ZDB Id: 506609-8
    ZDB Id: 514305-6
    ZDB Id: 505872-7
    ZDB Id: 1479266-7
    ZDB Id: 505867-3
    ZDB Id: 506259-7
    Standort Signatur Einschränkungen Verfügbarkeit
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    Online-Ressource
  • 3
    Online-Ressource
    Online-Ressource
    Framework‐Hotspot Enhanced Trans Cleavage of CRISPR‐Cas12a for Clinical Samples Detection
    Wiley ; 2023
    In:  Angewandte Chemie International Edition Vol. 62, No. 32 ( 2023-08-07)
    Details
    In: Angewandte Chemie International Edition, Wiley, Vol. 62, No. 32 ( 2023-08-07)
    Kurzfassung: The trans‐cleavage property of CRISPR‐Cas12a system makes it an excellent tool for disease diagnosis. Nevertheless, most methods based on CRISPR‐Cas system still require pre‐amplification of the target to achieve the desired detection sensitivity. Here we generate Framework‐Hotspot reporters (FHRs) with different local densities to investigate their effect on trans‐cleavage activity of Cas12a. We find that the cleavage efficiency increases and the cleavage rate accelerates with increasing reporter density. We further construct a modular sensing platform with CRISPR‐Cas12a‐based target recognition and FHR‐based signal transduction. Encouragingly, this modular platform enables sensitive (100 fM) and rapid ( 〈 15 min) detection of pathogen nucleic acids without pre‐amplification, as well as detection of tumor protein markers in clinical samples. The design provides a facile strategy for enhanced trans cleavage of Cas12a, which accelerates and broadens its applications in biosensing.
    Materialart: Online-Ressource
    ISSN: 1433-7851 , 1521-3773
    URL: Issue
    URL: Article
    DOI: 10.1002/anie.v62.32
    DOI: 10.1002/anie.202305536
    RVK:
    VA 2100
    Sprache: Englisch
    Verlag: Wiley
    Publikationsdatum: 2023
    ZDB Id: 2011836-3
    ZDB Id: 123227-7
    Standort Signatur Einschränkungen Verfügbarkeit
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    Online-Ressource
  • 4
    Online-Ressource
    Online-Ressource
    Ultrasensitive DNA‐Biomacromolecule Sensor for the Detection Application of Clinical Cancer Samples
    Wiley ; 2022
    In:  Advanced Science Vol. 9, No. 6 ( 2022-02)
    Details
    In: Advanced Science, Wiley, Vol. 9, No. 6 ( 2022-02)
    Kurzfassung: Diagnostic testing of biological macromolecules is of great significance for early warning of disease and cancer. Nevertheless, restricted by limited surface area and large steric hindrance, sensitive detection of macromolecules with interface‐based sensing method remains challenging. Here, a “biphasic replacement” electrochemical aptamer‐based (BRE‐AB) sensing strategy which placed capture reaction of the biomacromolecule in a homogeneous solution phase and replaced with a small diameter of single‐stranded DNA to attach to the interface is introduced. Using the BRE‐AB sensor, the ultrasensitive detection of luteinizing hormone (LH) with the detection limit of 10 × 10 −12 m is demonstrated. Molecular Dynamics simulations are utilized to explore the binding mechanism of aptamer and target LH. Moreover, it is confirmed that the BRE‐AB sensor has excellent sensing performance in whole blood and undiluted plasma. Using the BRE‐AB sensor, the LH concentrations in 40 clinical samples are successfully quantified and it is found that LH is higher expressed in breast cancer patients. Furthermore, the sensor enables simple, low‐cost, and easy to regenerate and reuse, indicating potentially applicable for point‐of‐care biological macromolecules diagnostics.
    Materialart: Online-Ressource
    ISSN: 2198-3844 , 2198-3844
    URL: Issue
    URL: Article
    DOI: 10.1002/advs.v9.6
    DOI: 10.1002/advs.202102804
    Sprache: Englisch
    Verlag: Wiley
    Publikationsdatum: 2022
    ZDB Id: 2808093-2
    Standort Signatur Einschränkungen Verfügbarkeit
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    Online-Ressource
  • 5
    Online-Ressource
    Online-Ressource
    Facile way of preparing PMMA @ TiO 2 @ SiO 2 (P@T@S) composite microspheres with high haze and light transmittance for diffusion film
    Wiley ; 2024
    In:  Journal of Applied Polymer Science Vol. 141, No. 31 ( 2024-08-15)
    Details
    In: Journal of Applied Polymer Science, Wiley, Vol. 141, No. 31 ( 2024-08-15)
    Kurzfassung: In recent years, optical diffusion film materials with high transmittance and high haze have been widely reported, while there is relatively rare research on their functionalization and practical application especially in real scenario. In this work, optical diffusion films using polymethyl methacrylate (PMMA)@TiO 2 @SiO 2 (P@T@S) composite microspheres were fabricated, which exhibited sufficiently high light transmittance and haze. The binding mode of PMMA microspheres and nanoparticles suggested that PMMA microspheres with a positive surface potential combined with nanoparticles with a negative surface potential through electrostatic attraction to achieve mutual binding. The prepared P@T@S composite microspheres showed better heat and swelling resistance than those of PMMA microspheres. The P@T@S diffusion film, whose excellent light diffusion effect was clearly demonstrated, exhibited the best optical performance among all optical diffusion films prepared. After passing through the P@T@S diffusion film, the laser light was not only uniform but also bright. The findings from this work can provide some insight into further research on optical diffusion films applied in optoelectronic device areas.
    Materialart: Online-Ressource
    ISSN: 0021-8995 , 1097-4628
    URL: Issue
    URL: Article
    DOI: 10.1002/app.v141.31
    DOI: 10.1002/app.55738
    Sprache: Englisch
    Verlag: Wiley
    Publikationsdatum: 2024
    ZDB Id: 1491105-X
    Standort Signatur Einschränkungen Verfügbarkeit
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    Online-Ressource
  • 6
    Online-Ressource
    Online-Ressource
    Grazing by large herbivores improves soil microbial metabolic activity in a meadow steppe
    Wiley ; 2021
    In:  Grassland Science Vol. 67, No. 1 ( 2021-01), p. 30-40
    Details
    In: Grassland Science, Wiley, Vol. 67, No. 1 ( 2021-01), p. 30-40
    Kurzfassung: Large herbivores grazing is a major disturbance that can cause significant changes of soil environment in grassland ecosystems. However, it remains unclear how soil microbial metabolic activity responses to different grazing intensities. We analyzed the relationships between soil microbial carbon source utilization and grazing intensity, and further assessed the main factors determining soil microbial metabolic activity in a meadow steppe. Soil samples were analyzed along different grazing intensities (no grazing, light grazing and heavy grazing) with community‐level physiological profiles (CLPP) using Biolog Eco‐plates incubation to estimate soil microbial carbon source utilization patterns. Redundancy analysis (RDA) was performed to explore the major factors influencing soil microbial metabolic activity in a five‐year grazing grassland in northeast of China. Grazing significantly improved soil microbial community carbon utilization and increased utilization of carbohydrates, amino acids, phenolic acids and amines, while significantly decreased utilization of carboxylic acids. Among edaphic properties and plant traits, plant density, total biomass (TB), soil water content (SW) and C:N ratio (C/N) were main driving forces contributing to the carbon source utilization structure of the soil microbial communities. Soil microbial metabolic activity was promoted by grazing through altering plant traits and abiotic soil properties, and soil‐related factors were primary and direct driving force for soil microbial metabolic activity in grasslands of northeast China. The present study demonstrated differential soil microbial responses along grazing intensities and has important applications for better management practices in the grassland ecosystem.
    Materialart: Online-Ressource
    ISSN: 1744-6961 , 1744-697X
    URL: Issue
    URL: Article
    DOI: 10.1111/grs.v67.1
    DOI: 10.1111/grs.12282
    Sprache: Englisch
    Verlag: Wiley
    Publikationsdatum: 2021
    ZDB Id: 2186539-5
    Standort Signatur Einschränkungen Verfügbarkeit
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    Online-Ressource
  • 7
    Online-Ressource
    Online-Ressource
    Whole‐exome sequencing identifies rare pathogenic and candidate variants in sporadic Chinese Han deaf patients
    Wiley ; 2020
    In:  Clinical Genetics Vol. 97, No. 2 ( 2020-02), p. 352-356
    Details
    In: Clinical Genetics, Wiley, Vol. 97, No. 2 ( 2020-02), p. 352-356
    Kurzfassung: Genetic causes of hearing loss are highly heterogeneous and often ethnically specific. In recent years, a variety of next‐generation sequencing (NGS) panels have been developed to target deafness‐causative genes. Whole‐exome sequencing (WES), on the other hand, was rarely used for genetic testing for deafness. In this study, we performed WES in 38 sporadic Chinese Han deaf patients who have been pre‐excluded for mutations in common deafness genes GJB2 , SLC26A4 and MT‐RNR1 . Non‐synonymous variants have been filtered based on their minor allele frequencies in public databases and ethnically matched controls. Bi‐allelic pathogenic mutations in eight deafness genes, OTOF, TRIOBP, ESPN, HARS2, CDH23, MYO7A, USH1C and TJP2 , were identified in 10 patients, with 17 mutations identified in this study not being associated with deafness previously. For the rest 28 patients, possibly bi‐allelic rare non‐synonymous variants in an averaged 4.7 genes per patient were identified as candidate pathogenic causes for future analysis. Our study showed that WES may provide a unified platform for genetic testing of deafness and enables retro‐analyzing when new causative genes are revealed.
    Materialart: Online-Ressource
    ISSN: 0009-9163 , 1399-0004
    URL: Issue
    URL: Article
    DOI: 10.1111/cge.v97.2
    DOI: 10.1111/cge.13638
    RVK:
    WA 15000
    Sprache: Englisch
    Verlag: Wiley
    Publikationsdatum: 2020
    ZDB Id: 2004581-5
    Standort Signatur Einschränkungen Verfügbarkeit
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    Online-Ressource
  • 8
    Online-Ressource
    Online-Ressource
    Research focus and thematic trends in magnet hospital research: A bibliometric analysis of the global publications
    Wiley ; 2021
    In:  Journal of Advanced Nursing Vol. 77, No. 4 ( 2021-04), p. 2012-2025
    Details
    In: Journal of Advanced Nursing, Wiley, Vol. 77, No. 4 ( 2021-04), p. 2012-2025
    Kurzfassung: 本研究旨在对磁性医院在1988‐2020年间的研究进行文献计量分析。 设计 2020年2月至5月期间, 进行统计和可视化文献计量分析。 方法 通过主题高级搜索策略从科学网中提取信息。利用HistCite‐Pro、Excel、GunnMap、BibExcel和VOSviewer提取、整合和可视化文献计量信息。此外, 回顾已发表的论文, 依此对核心作者的研究内容进行探讨和总结。 结果 1988‐2020年间, 在科学网上检索出107种期刊上发表的文章 ( N =396) 。美国 ( N =319) 和宾夕法尼亚大学 ( N =38) 分别是期刊发表数最多的国家和机构。根据多个作者引用和文献耦合网络分析, 可得出结论: Aiken、Kramer、Laschinger、Schmalenberg、Lake和Johantgen是这一领域的主要贡献者和领导者。根据主要贡献者的研究内容和关键词共现分析结果, 研究重点可划分为四个部分, 分别是: 造成磁性医院结果差异的机制、循证实践、磁性医院与非磁性医院疗效比较以及实践环境。 结论 本文献计量学研究对磁性医院的研究现状进行全面综述, 并且, 这一领域受到越来越多的关注。然而, 目前国内外对磁性医院的研究相对有限, 需要各方进一步重视, 特别是发展中国家的重视。 影响 本研究显示了贡献最大的国家、期刊、机构、作者以及与磁性医院相关的主要主题, 有助于研究者寻找潜在的合作伙伴, 优化和深化未来研究内容, 例如, 加强发展中国家磁性医院的研究, 提高循证研究的水平, 考虑不同文化造成的影响等。此外, 可通过本研究为医院政策制定者提供建议。
    Materialart: Online-Ressource
    ISSN: 0309-2402 , 1365-2648
    URL: Issue
    URL: Article
    DOI: 10.1111/jan.v77.4
    DOI: 10.1111/jan.14757
    Sprache: Englisch
    Verlag: Wiley
    Publikationsdatum: 2021
    ZDB Id: 2009963-0
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 9
    Online-Ressource
    Online-Ressource
    Triple‐Asymmetric Flexible Strain Sensor Based on High‐aligned Ni Nanowire Arrays with Opposite Resistance Responding for Multidimensional Motions Detection
    Wiley ; 2023
    In:  Advanced Materials Technologies Vol. 8, No. 2 ( 2023-01)
    Details
    In: Advanced Materials Technologies, Wiley, Vol. 8, No. 2 ( 2023-01)
    Kurzfassung: Herein, a triple‐asymmetric flexible strain sensor by using the single‐layered high‐aligned Ni‐nanowire arrays is proposed for the applications of monitoring multidimensional motions of human joints. The triple‐asymmetric structures consist of a spatially ordering distribution of Ni nanowires (NWs) and double position asymmetric distributions of the Ni NWs in width and thickness direction of sensor. Because Ni NWs are isolated from each other, the resistance change of the sensor is by tunneling effect. The resistance values of the proposed sensor decrease with the increasing of stretching strain, which is different from that of the common flexible strain sensors, and the maximum absolute valve of gauge factor is 35. Furthermore, the proposed sensor has a good bending sensitivity, ranging from 90° to −90°, and minimum resolution angle of 5° in out‐plane bending. Benefiting from the asymmetric structures and tunneling effect, the complex movements of human joints (such as up‐down bending, left‐right swing, and rotating of wrist) are successfully detected. The practical applicability in real‐time monitoring and the processes of handwriting are also shown. Therefore, this proposed sensor is believed to have great potential in future development of micro‐motion monitoring, wearable electronics, and artificial intelligence.
    Materialart: Online-Ressource
    ISSN: 2365-709X , 2365-709X
    URL: Issue
    URL: Article
    DOI: 10.1002/admt.v8.2
    DOI: 10.1002/admt.202201093
    Sprache: Englisch
    Verlag: Wiley
    Publikationsdatum: 2023
    ZDB Id: 2850995-X
    Standort Signatur Einschränkungen Verfügbarkeit
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    Online-Ressource
  • 10
    Online-Ressource
    Online-Ressource
    Face‐to‐Face Orientation of Quasiplanar Donor and Acceptor Enables Highly Efficient Intramolecular Exciplex Fluorescence
    Wiley ; 2021
    In:  Angewandte Chemie International Edition Vol. 60, No. 8 ( 2021-02-19), p. 3994-3998
    Details
    In: Angewandte Chemie International Edition, Wiley, Vol. 60, No. 8 ( 2021-02-19), p. 3994-3998
    Kurzfassung: Intramolecular through‐space charge‐transfer (TSCT) excited states have been exploited for developing thermally activated delayed fluorescence (TADF) emitters, but the tuning of excited state dynamics by conformational engineering remains sparse. Designed here is a series of TSCT emitters with precisely controlled alignment of the donor and acceptor segments. With increasing intramolecular π–π interactions, the radiative decay rate of the lowest singlet excited state (S 1 ) progressively increased together with a suppression of nonradiative decay, leading to significantly enhanced photoluminescence quantum yields of up to 0.99 in doped thin films. A high‐efficiency electroluminescence device, with a maximum external quantum efficiency (EQE) of 23.96 %, was achieved and maintains 〉 20 % at a brightness of 1000 cd m −2 . This work sheds light on the importance of conformation control for achieving high‐efficiency intramolecular exciplex emitters.
    Materialart: Online-Ressource
    ISSN: 1433-7851 , 1521-3773
    URL: Issue
    URL: Article
    DOI: 10.1002/anie.v60.8
    DOI: 10.1002/anie.202013051
    RVK:
    VA 2100
    Sprache: Englisch
    Verlag: Wiley
    Publikationsdatum: 2021
    ZDB Id: 2011836-3
    ZDB Id: 123227-7
    Standort Signatur Einschränkungen Verfügbarkeit
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    Online-Ressource
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