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  • Wiley  (16)
  • 2020-2024  (16)
  • 1
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    Berry transcriptome: insights into a novel resource to understand development dependent secondary metabolism in Withania somnifera (Ashwagandha)
    Wiley ; 2020
    In:  Physiologia Plantarum Vol. 168, No. 1 ( 2020-01), p. 148-173
    Details
    In: Physiologia Plantarum, Wiley, Vol. 168, No. 1 ( 2020-01), p. 148-173
    Abstract: Withania somnifera (Ashwagandha) is considered as Rasayana in Indian systems of medicine. This study reports a novel transcriptome of W. somnifera berries, with high depth, quality and coverage. Assembled and annotated transcripts for nearly all genes related with the withanolide biosynthetic pathway were obtained. Tissue‐wide gene expression analysis reflected almost similar definitions for the terpenoid pathway in leaf, root and berry tissues with relatively higher abundance of transcripts linked to steroid, phenylpropanoid metabolism as well as flavonoid metabolism in berries. The metabolome map generated from the data embodied transcripts from 143 metabolic pathways connected together and mediated collectively by about 1792 unique enzyme functions specific to berry, leaf and root tissues, respectively. Transcripts specific to cytochrome p450 (CYP450), methyltransferases and glycosyltransferases were distinctively located in a tissue specific manner and exhibited a complex network. Significant distribution of transcription factor genes such as MYB, early light inducible protein (ELI), minichromosome maintenance1, agamous, deficiens and serum response factor (MADS) and WRKY etc. was observed, as the major transcriptional regulators of secondary metabolism. Validation of the assembly was ascertained by cloning WsELI , which has a light dependent regulatory role in development. Quantitative expression of WsELI was observed to be considerably modulated upon exposure to abiotic stress and elicitors. Co‐relation of over‐expression of WsELI , may provide new aspects for the functional role of ELI proteins in plants linked to secondary metabolism. The study offers the first comprehensive and comparative evaluation of W. somnifera transcriptome data between the three tissues and across other members of Solanaceae ( Nicotiana , Solanum and Capsicum ) with respect to major pathways and their metabolome regulation.
    Type of Medium: Online Resource
    ISSN: 0031-9317 , 1399-3054
    URL: Issue
    URL: Article
    DOI: 10.1111/ppl.v168.1
    DOI: 10.1111/ppl.12943
    RVK:
    WA 15000
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 208872-1
    detail.hit.zdb_id: 2020837-6
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  • 2
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    Increased DNA methylation in the spermatogenesis‐associated (SPATA) genes correlates with infertility
    Wiley ; 2020
    In:  Andrology Vol. 8, No. 3 ( 2020-05), p. 602-609
    Details
    In: Andrology, Wiley, Vol. 8, No. 3 ( 2020-05), p. 602-609
    Abstract: Spermatogenesis‐associated (SPATA) family of genes plays important roles in spermatogenesis, sperm maturation or fertilization. The knockout studies in mice have demonstrated that SPATA genes are crucial for fertility. Gene expression and genetic polymorphism studies have further suggested their correlation with infertility; however, methylation analysis of SPATA genes in human male infertility has not yet been undertaken. Objectives To analyze the methylation status of SPATA4, SPATA5 and SPATA6 genes in oligozoospermic male infertility. Materials and methods In the present study, we have analyzed DNA methylation pattern in the promoter regions of SPATA4, SPATA5 and SPATA6 genes in oligozoospermic patients and compared it with normozoospermic fertile controls. Semen samples were obtained from 30 oligozoospermic infertile and 19 normozoospermic fertile controls, and DNA methylation levels of the target gene promoters were analyzed by amplicon based deep sequencing methylation analysis using MiSeq. Results SPATA4 ( P   〈  0.0008), SPATA5 ( P  = 0.009) and SPATA6 (Promoter, P   〈  0.0005; Exon 1, P  = 0.0128) genes were significantly hypermethylated in oligozoospermic patients in comparison to controls. This is the first study reporting a higher methylation in the promoters of SPATA4, SPATA5 and SPATA6 in oligozoospermic infertile individuals in comparison to the normozoospermic fertile controls. Discussion Altered methylation of SPATA genes would affect pathways involved in sperm production or affect various processes linked to sperm fertility. Conclusion In conclusion, hypermethylation in the SPATA4, SPATA5 and SPATA6 genes correlates with oligozoospermic infertility.
    Type of Medium: Online Resource
    ISSN: 2047-2919 , 2047-2927
    URL: Issue
    URL: Article
    DOI: 10.1111/andr.v8.3
    DOI: 10.1111/andr.12742
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 2693844-3
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  • 3
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    Pressmud alleviates soil sodicity stress in a rice–wheat rotation: Effects on soil properties, physiological adaptation and yield‐related traits
    Wiley ; 2021
    In:  Land Degradation & Development Vol. 32, No. 9 ( 2021-05-30), p. 2735-2748
    Details
    In: Land Degradation & Development, Wiley, Vol. 32, No. 9 ( 2021-05-30), p. 2735-2748
    Abstract: We assessed the improvement potential of pressmud – a sugar industry waste – on soil properties, physiological adaptation and yield‐related traits in a rice–wheat rotation in sodicity‐affected Ghaghar basin of Haryana, India. Large‐scale (0.2–0.4 ha) participatory trials comprising two treatments, with (+PM) and without (–PM) pressmud, were conducted on 125 farmers' fields covering non‐sodic (NS), slightly sodic (SS) and moderately sodic (MS) soils. Pressmud application (10 Mg ha −1 ) led to appreciable reductions in soil pH (1.6–3.6%) and exchangeable sodium percentage (ESP; 10.4–20.1%) with concomitant improvements in plant physiological and yield‐related traits across different soil alkali classes, albeit to a greater extent in SS and MS soils than in NS soils. Compared to prevailing farmers' practices (–PM), transformative improvements in mean leaf relative water content (RWC) (4 and 7%), membrane injury (MII) (−12 and −14%), photosynthetic rate (Pn) (21 and 25%), stomatal conductance (gS) (26 and 21%), transpiration rate (E) (38 and 22%), proline (P) (−19 and −20%) and Na + /K + accumulation in shoot (NaK_S) (−24 and −22%) and root (NaK_R) (−23 and −27%) were observed in both rice and wheat grown under PM ameliorated soils. On an average, rice and wheat yields were increased by 15.8 and 14.6% in SS and 18.9 and 16.7% in MS soils, respectively, with pressmud application. Principal Component Analysis (PCA) showed significant correlations among highly weighted variables including physiological and yield‐related traits to influence the rice–wheat productivity with PM addition in SS and MS soils. Sustained use of this organic waste in agricultural lands seems to be an affordable solution for overcoming sodicity‐induced land degradation, and achieving developmental goals of environmental and livelihood security.
    Type of Medium: Online Resource
    ISSN: 1085-3278 , 1099-145X
    URL: Issue
    URL: Article
    DOI: 10.1002/ldr.v32.9
    DOI: 10.1002/ldr.3953
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 2021787-0
    detail.hit.zdb_id: 1319202-4
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  • 4
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    Improved Power Conversion Efficiencies of Dye‐Capped and Sensitized ZnO Solar Cells
    Wiley ; 2022
    In:  ChemistrySelect Vol. 7, No. 38 ( 2022-10-13)
    Details
    In: ChemistrySelect, Wiley, Vol. 7, No. 38 ( 2022-10-13)
    Abstract: This article describes the synthesis, characterization and photo‐physical studies of ZnO nanoparticles capped with monomer and polymer dyes D 1 and D 2 , respectively; these dyes were synthesized by an easy and inexpensive condensation method by reacting Indole‐3‐carboxaldehyde with N,N‐ dimethylethylenediamine and polyamine. The optoelectronic properties of these dyes were investigated by UV‐Vis spectroscopy. Further, these dyes D1 and D2 were decorated with ZnO nanoparticles and labelled as samples S 1 and S 2 . The structural properties of dyes capped ZnO nanoparticles were characterized by X‐ray diffractogram, scanning electron microscopy, and dynamic light scattering studies. Further, S 1 and S 2 were deployed to fabricate photo‐anodes for ZnO solar cells, labelled as SC 1 and SC 2 , respectively. The results of investigations revealed that polymer dye molecules enhanced the photo‐harvesting capability of ZnO nanoparticles within the ZnO solar cell SC 1 . Maximum photo‐energy conversion efficiency η =5.42 % was attained with solar cell SC 2 under AM 1.5 solar irradiations. Comparatively, it has been observed that the recorded photovoltaic efficiency of solar cell SC 2 was enhanced by 1.49 % comparative to the solar cell SC 1 . The IPCE spectra studies revealed a significant improvement of (15–19)% in the photo efficiency of solar cell SC 2 than SC 1.
    Type of Medium: Online Resource
    ISSN: 2365-6549 , 2365-6549
    URL: Issue
    URL: Article
    DOI: 10.1002/slct.v7.38
    DOI: 10.1002/slct.202202075
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 2844262-3
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  • 5
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    Mononuclear luminous β‐diketonate Ln(III) complexes with heteroaromatic auxiliary ligands: synthesis and luminescence characteristics
    Wiley ; 2022
    In:  Luminescence Vol. 37, No. 11 ( 2022-11), p. 1921-1931
    Details
    In: Luminescence, Wiley, Vol. 37, No. 11 ( 2022-11), p. 1921-1931
    Abstract: A series of lanthanide (samarium and terbium) β‐diketonates with heteroaromatic auxiliary ligands was synthesized. The prepared complexes were characterized through electrochemical, thermal, and spectroscopic analyses. Infrared analysis revealed the binding of the respective metal ion to oxygen and nitrogen atoms of diketone and ancillary ligands. Thermogravimetry/differential thermogravimetry profiles provided thermal information and specified the high thermal stability of the prepared complexes. The complexes exhibited the sharp and structured Ln‐based emission in the visible region upon irradiation in the ultraviolet range. Photophysical analysis demonstrated the green and orange‐red emission due to the respective characteristic transitions of Tb 3+ and Sm 3+ ions. Photophysical properties affirmed the luminous behaviour of the synthesized complexes. These luminous lanthanide complexes could be used as emitting materials in the design of organic light‐emitting diodes.
    Type of Medium: Online Resource
    ISSN: 1522-7235 , 1522-7243
    URL: Issue
    URL: Article
    DOI: 10.1002/bio.v37.11
    DOI: 10.1002/bio.4376
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 2001819-8
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  • 6
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    Red emissive ternary europium complexes: synthesis, optical, and luminescence characteristics
    Wiley ; 2022
    In:  Luminescence Vol. 37, No. 8 ( 2022-08), p. 1309-1320
    Details
    In: Luminescence, Wiley, Vol. 37, No. 8 ( 2022-08), p. 1309-1320
    Abstract: Solid ternary europium complexes consisting of fluorinated β‐diketone (thenoyltrifluoroacetone, TTFA) and heteroaromatic bidentate auxiliary ligands were synthesized. The luminescence features of the complexes were estimated using various spectral measurements and clearly proved that the Eu 3+ ion is efficiently sensitized by ligands by an antenna effect. Photoluminescence excitation spectra have shown that Eu(III) complexes are excited effectively in the ultraviolet (UV) region and the corresponding emission spectra consist of characteristic peaks attributed to the 5 D 0 → 7 F J transitions of the europium ion with the strongest emission peak at 611 nm ( 5 D 0 → 7 F 2 ). From photoluminescence (PL) data, decay time, Judd–Ofelt parameters, transition rates, and quantum efficiency of the complexes were also determined. The Commission Internationale de l'éclairage (CIE) colour coordinates indicated the bright red emission of ternary europium complexes. Correlated colour temperature values indicated the utilization of these complexes in display devices. Judd–Ofelt and photophysical parameters were also estimated theoretically using LUMPAC software. Various frontier molecular orbitals and their respective energy were determined. These red emissive europium complexes could be utilized for fabricating solid‐state lighting systems.
    Type of Medium: Online Resource
    ISSN: 1522-7235 , 1522-7243
    URL: Issue
    URL: Article
    DOI: 10.1002/bio.v37.8
    DOI: 10.1002/bio.4297
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 2001819-8
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  • 7
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    Altered cord serum 25‐hydroxyvitamin D signaling and placental inflammation is associated with pre‐term birth
    Wiley ; 2020
    In:  American Journal of Reproductive Immunology Vol. 83, No. 2 ( 2020-02)
    Details
    In: American Journal of Reproductive Immunology, Wiley, Vol. 83, No. 2 ( 2020-02)
    Abstract: Vitamin D is well‐known for having anti‐inflammatory and immunomodulatory properties. Impaired maternal vitamin D status has been known to increase the risk of adverse pregnancy outcomes like pre‐term birth. The present study aims to evaluate the impact of fetal cord serum 25‐hydroxyvitamin D‐mediated signaling in mediating inflammatory responses in placenta during pre‐term birth. Method of study For the above purpose, cord serum 25 hydroxyvitamin D 25(OH)D were measured in term (n = 20) and pre‐term (n = 20) born babies using ELISA. Vitamin D downstream signaling has also been checked in placenta (VDR, CYP27B1, cathelicidin LL37) along with expression of inflammatory markers (S100A8, HMGB1, TLR2, p‐NF‐kappaB) using Western blotting and immunohistochemistry. Pearson correlation model was used to do correlation study. Results Compared with term born babies (59.31 ± 3.476), decline in cord serum 25(OH)D levels is observed in pre‐term born babies (22.26 ± 1.083, P  =  〈 0.0001) that showed strong positive correlation with gestational age ( r  = .9368***) and birthweight ( r  = .9559***). On the other hand, vitamin D signaling markers were found to be downregulated and inflammatory markers were upregulated in placental tissue of pre‐term born babies. Conclusion Thus, our study demonstrated that insufficient cord 25(OH)D levels may disturb the homeostasis of inflammation in placenta. Altered cord serum 25(OH)D mediated anti‐inflammatory signaling may be acting as trigger signals in modulating inflammatory responses in placenta and eliciting premature activation of spontaneous labor in pre‐term birth.
    Type of Medium: Online Resource
    ISSN: 1046-7408 , 1600-0897
    URL: Issue
    URL: Article
    DOI: 10.1111/aji.v83.2
    DOI: 10.1111/aji.13201
    RVK:
    XA 20240
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 2024667-5
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  • 8
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    Synthesis of green emissive terbium(III) complexes for displays: Optical, electrochemical and photoluminescent analysis
    Wiley ; 2023
    In:  Luminescence Vol. 38, No. 1 ( 2023-01), p. 56-63
    Details
    In: Luminescence, Wiley, Vol. 38, No. 1 ( 2023-01), p. 56-63
    Abstract: A series of heteroleptic terbium(III) complexes with fluorinated 2‐thenoyltrifluoroacetone (TTFA) and other heteroaromatic units have been synthesized. The developed heteroleptic complexes were inspected via elemental study, cyclic voltammetry, thermal analysis and spectroscopic investigations. Optical band‐gap data proposed the conducting property of prepared complexes. The photoluminescence emission profiles illustrated peaks based on terbium(III) cation (Tb 3+ ) positioned at ~617, 586, 546 and 491 nm, imputed to 5 D 4 to 7 F J ( J  = 3,4,5,6) transitions separately. Most intense peak at 546 nm corresponding to 5 D 4  →  7 F 5 transition is accountable for the green emissive character of developed complexes. The luminous character of complexes reveals the sensitization of Tb 3+ by ligands. Color parameters further corroborates the green emanation of Tb 3+ complexes. The photometric characteristics of complexes recommended their usages in designing display devices.
    Type of Medium: Online Resource
    ISSN: 1522-7235 , 1522-7243
    URL: Issue
    URL: Article
    DOI: 10.1002/bio.v38.1
    DOI: 10.1002/bio.4423
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2001819-8
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  • 9
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    Inflammation driven tumor‐like signaling in prostatic epithelial cells by sexually transmitted Trichomonas vaginalis
    Wiley ; 2021
    In:  International Journal of Urology Vol. 28, No. 2 ( 2021-02), p. 225-240
    Details
    In: International Journal of Urology, Wiley, Vol. 28, No. 2 ( 2021-02), p. 225-240
    Abstract: To identify the sequence of inflammation‐driven signaling cascades and other molecular events that might cause tumor‐like transformation of prostatic cells. Methods Cytokine array analysis, Reactome and STRING analysis, immunoblotting, and immunocytochemistry were used to investigate the molecular mechanisms governing inflammation‐driven adverse changes in human prostatic cells caused by the sexually transmitted infection, Trichomonas vaginalis , resulting in prostatitis, benign prostatic hyperplasia and prostate cancer. Results Array analysis showed upregulation of 23 cytokines within 24 h of infection of human prostatic epithelial RWPE‐1 cells with the parasite, in vitro . Reactome and STRING analysis of array data identified interleukin‐6, interleukin‐8, nuclear factor kappa B, signal transducer and activator of transcription 3 and cyclooxygenase 2 as chief instigators of prostatic anomaly, which were found to be significantly upregulated by immunofluorescence and western blotting analyses. STRING further connected these instigators with macrophage migration inhibitory factor, PIM‐1 and prostate‐specific antigen; which was confirmed by their marked stimulation in infected prostatic cells by immunoblotting and immunocytochemistry. Upregulated proliferation markers, such as Ki67, proliferating cell nuclear antigen and B‐cell lymphoma 2, suggested tumor‐like signaling in infected RWPE‐1 cells, which was further supported by downregulation of E‐cadherin, upregulation of vimentin and activation of focal adhesion kinase. Prostate tumor DU145 cells were more sensitive to parasite invasion, and showed rapid upregulation with nuclear translocation of sensitive parameters, such as nuclear factor kappa B, signal transducer and activator of transcription 3, and macrophage migration inhibitory factor. The migration of DU145 cells augmented when incubated in spent media from parasite‐infected RWPE‐1 cells. Conclusion The initiation of inflammation driven tumor‐like cell signaling in parasite‐infected human prostatic epithelial cells is apparent, with the prostate tumor (DU145) cells being more sensitive to T. vaginalis than normal (RWPE‐1) prostatic cells.
    Type of Medium: Online Resource
    ISSN: 0919-8172 , 1442-2042
    URL: Issue
    URL: Article
    DOI: 10.1111/iju.v28.2
    DOI: 10.1111/iju.14431
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 2009793-1
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  • 10
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    Exploring the genetic variation for sodicity tolerance in Melia dubia evolved in Indian conditions
    Wiley ; 2022
    In:  Land Degradation & Development Vol. 33, No. 1 ( 2022-01-15), p. 41-54
    Details
    In: Land Degradation & Development, Wiley, Vol. 33, No. 1 ( 2022-01-15), p. 41-54
    Abstract: Soil sodicity is one of the major constraints for the success of afforestation worldwide. High sodicity causes poor soil physical conditions and thus adversely affect tree growth and biomass production. However, considerable intraspecific variation for sodicity tolerance exists in most of the trees species. Presently, no information is available on sodicity tolerance in the genotypes of the industrially important Melia dubia tree. Therefore, we examined twenty‐five genotypes of M. dubia, that has evolved in different parts of India, to elucidate mechanisms and identify traits imparting sodicity tolerance in these genotypes. The experimental evidence showed the significant genetic variability and relationships in genotypes for various morphological and physiological traits in response to the sodicity stress. On an average, sodicity stress reduced plant height, collar diameter, and shoot biomass by 15%, 18%, and 60%, respectively. The shoots Na + concentration had negative correlation with the growth traits. Shoot Na + ions exclusion was found to increase sodicity tolerance and conferred salt tolerance mechanism in M. dubia . Path analysis revealed that the plant height, collar diameter, number of leaves, and branches were major determinants of shoot biomass production. Cluster analysis validated the genotypes response to sodicity and classified the germplasm into sensitive, moderately tolerant, and tolerant category each consisting of nine, eleven, and five genotypes, respectively. Among genotypes, the MDSS06, MDSS13, and MDSS21 produced greater growth and biomass under sodic stress and indicated good growth potential in such soil. The results showed the presence of a high genetic diversity for salt tolerance in Melia , and the identified genotypes can be considered for strengthening the breeding programs aiming at improving the sodicity tolerance of the species. Our result concluded that genetic variation and breeding approaches using the MDSS06, 13, and 21 genotypes have tremendous potential to improve the growth, biomass, and overall productivity of M. dubia under sodic soil conditions.
    Type of Medium: Online Resource
    ISSN: 1085-3278 , 1099-145X
    URL: Issue
    URL: Article
    DOI: 10.1002/ldr.v33.1
    DOI: 10.1002/ldr.4126
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 2021787-0
    detail.hit.zdb_id: 1319202-4
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