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  • Wiley  (38)
  • 2020-2024  (38)
  • 1
    In: Veterinary Medicine and Science, Wiley, Vol. 7, No. 5 ( 2021-09), p. 1999-2007
    Abstract: Gonadotropin‐releasing hormone (GnRH) plays a pivotal role in regulating the reproductive endocrine system. Objective An immunocontraception vaccine aimed at inhibiting the functions of GnRH is tested as a potential tool for controlling animal populations. Methods We developed a recombinant immunocontraceptive vaccine composed of GnRH‐I and GnRH‐II (GnRH I+II), which was conjugated with Salmonella typhimurium flagellin. Forty‐eight BALB/c mice aged 4 weeks were divided into four groups (each group had n  = 12): non‐vaccinated male (NVM), non‐vaccinated female (NVF), vaccinated male (VM), and vaccinated female (VF). Mice in the vaccinated groups were vaccinated twice by intramuscular injection at 0 and 2 weeks with 300 μg of the recombinant GnRH protein complex per mouse. Mice in the non‐vaccinated groups were injected with saline and served as the unimmunized controls. Twenty‐four pairs of male and female mice were mated for 10–12 weeks after initial immunization in four groups: 6 NVF × 6 NVM, 6 VF × 6 NVM, 6 NVF × 6 VM, and 6 VF × 6 VM. Results: An increase ( p   〈  0.001) in antibody titers in VM and VF mice was observed. The testosterone levels and the number of spermatocytes were lower ( p   〈  0.001) in VM mice than those in the control mice. The progesterone levels and the number of corpora lutea were lower ( p   〈  0.001) than those in the control mice. Mating results in both VM and VF mice confirmed a 60% reduction in pregnancy rates and offspring numbers. Conclusions The recombinant GnRH vaccine can be used for birth control in both male and female animals.
    Type of Medium: Online Resource
    ISSN: 2053-1095 , 2053-1095
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 2819409-3
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  • 2
    In: Cancer Medicine, Wiley, Vol. 10, No. 12 ( 2021-06), p. 3964-3973
    Abstract: There is no proven primary preventive strategy for doxorubicin‐induced subclinical cardiotoxicity (DISC), especially among patients without a cardiovascular (CV) risk. We investigated the primary preventive effect on DISC of the concomitant use of angiotensin receptor blockers (ARBs) or beta‐blockers (BBs), especially among breast cancer patients without a CV risk. Methods A total of 385 patients who were scheduled for doxorubicin chemotherapy were screened. Among them, 195 patients of the study populations were included and were randomly divided into two groups [candesartan 4 mg q.d. vs. carvedilol 3.125 mg q.d.] and patients who were unwilling to take one of the medications were evaluated as controls. The primary outcomes were the incidence of early DISC (DISC developing within 6 months after chemotherapy), and late DISC (DISC developing only at least 12 months after chemotherapy). Result Compared with the control group (8 out of 43 patients (18.6%)), only the candesartan group (4 out of 82 patients (4.9%)) showed a significantly lower incidence of early DISC ( p  = 0.022). Compared with the control group, the candesartan group demonstrated a significantly reduced decrease in left ventricular ejection fraction (LVEF) throughout the study period [−1.0% vs. −3.00 ( p  〈  0.001) at the first follow‐up, −1.10% vs. −3.40( p =  0.009) at the second follow‐up]. Conclusions Among breast cancer patients without a CV risk treated with doxorubicin‐containing chemotherapy, subclinical cardiotoxicity is prevalent and concomitant administration of low‐dose candesartan might be effective to prevent an early decrease in LVEF. Further large‐scale, randomized controlled trials will be needed to confirm our findings.
    Type of Medium: Online Resource
    ISSN: 2045-7634 , 2045-7634
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 2659751-2
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  • 3
    In: International Journal of Imaging Systems and Technology, Wiley, Vol. 31, No. 3 ( 2021-09), p. 1087-1104
    Abstract: We aimed to evaluate the performance of convolutional neural networks (CNNs) in the classification of coronavirus disease 2019 (COVID‐19) disease using normal, pneumonia, and COVID‐19 chest radiographs (CXRs). First, we collected 9194 CXRs from open datasets and 58 from the Korea University Anam Hospital (KUAH). The number of normal, pneumonia, and COVID‐19 CXRs were 4580, 3884, and 730, respectively. The CXRs obtained from the open dataset were randomly assigned to the training, tuning, and test sets in a 70:10:20 ratio. For external validation, the KUAH (20 normal, 20 pneumonia, and 18 COVID‐19) dataset, verified by radiologists using computed tomography, was used. Subsequently, transfer learning was conducted using DenseNet169, InceptionResNetV2, and Xception to identify COVID‐19 using open datasets (internal) and the KUAH dataset (external) with histogram matching. Gradient‐weighted class activation mapping was used for the visualization of abnormal patterns in CXRs. The average AUC and accuracy of the multiscale and mixed‐COVID‐19Net using three CNNs over five folds were (0.99 ± 0.01 and 92.94% ± 0.45%), (0.99 ± 0.01 and 93.12% ± 0.23%), and (0.99 ± 0.01 and 93.57% ± 0.29%), respectively, using the open datasets (internal). Furthermore, these values were (0.75 and 74.14%), (0.72 and 68.97%), and (0.77 and 68.97%), respectively, for the best model among the fivefold cross‐validation with the KUAH dataset (external) using domain adaptation. The various state‐of‐the‐art models trained on open datasets show satisfactory performance for clinical interpretation. Furthermore, the domain adaptation for external datasets was found to be important for detecting COVID‐19 as well as other diseases.
    Type of Medium: Online Resource
    ISSN: 0899-9457 , 1098-1098
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 2009087-0
    SSG: 11
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  • 4
    In: Oral Diseases, Wiley, Vol. 28, No. S2 ( 2022-11), p. 2456-2464
    Abstract: Severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) entry into the host cells depends on the expression of angiotensin‐converting enzyme 2 (ACE2) and transmembrane protease serine 2 (TMPRSS2). We investigated the distribution of ACE2‐ and TMPRSS2‐expressing cells in various oral tissues to identify the underlying mechanism of oral manifestations in patients with coronavirus disease 2019. Subjects We analyzed the expression patterns of ACE2 and TMPRSS2 in the oral mucosa (tongue, palate, and buccal mucosa), trigeminal ganglion, vessels, and salivary glands of 9 Sprague‐Dawley rats using immunohistochemistry and immunofluorescence. Results ACE2 and TMPRSS2 were strongly expressed in the intermediate layer of the squamous epithelia of tongue papillae and buccal mucosa. ACE2‐ and TMPRSS2‐positive cells were observed in the taste buds of the tongue. Additionally, ACE2 and TMPRSS2 were co‐expressed in the ductal epithelium and acinar cells of salivary glands. Furthermore, both ACE2 and TMPRSS2 were stained in the neuronal cell body of trigeminal ganglia, but not in Schwann cells. Moreover, ACE2 and TMPRSS2 were expressed in capillaries, but not in venules/arterioles. Conclusions SARS‐CoV‐2 can spread the suprabasal area of squamous epithelia of the oral mucosa, invades taste bud, trigeminal nerve, parotid gland, and microvessel, resulting in oral manifestations.
    Type of Medium: Online Resource
    ISSN: 1354-523X , 1601-0825
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 2008428-6
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  • 5
    Online Resource
    Online Resource
    Wiley ; 2020
    In:  Pain Practice Vol. 20, No. 4 ( 2020-04), p. 396-398
    In: Pain Practice, Wiley, Vol. 20, No. 4 ( 2020-04), p. 396-398
    Abstract: Lumbosacral radicular pain with or without radiculopathy is a fairly common clinical condition. In such cases, lumbosacral transforaminal epidural injection (TFEI) is one of the most commonly performed interventions. Recent studies have explored the effectiveness of ultrasound (US)‐guided lumbosacral TFEI. Most of these studies have focused on lumbar TFEI via an in‐plane approach. S1 TFEI can also be easily performed, since the S1 foramen is more superficial than the lumbar foramen. However, most studies regarding US‐guided sacral TFEI use an out‐of‐plane technique. Therefore, in this report we present a video of US‐guided S1 TFEI via an in‐plane approach.
    Type of Medium: Online Resource
    ISSN: 1530-7085 , 1533-2500
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 2046672-9
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  • 6
    In: The Laryngoscope, Wiley, Vol. 130, No. 3 ( 2020-03), p. 732-740
    Abstract: The role of sex hormones for voice changes in men and women is presently unknown. To determine the effect of sex hormone on the vocal fold, changes of the extracellular matrix (ECM) in vocal fold lamina propria were assessed in orchiectomized (ORX) and ovariectomized (OVX) rats. Methods Male and female Sprague‐Dawley rats were divided into sham‐operated control male (CON‐ORX), ORX, sham‐operated female (CON‐OVX), and OVX rats. Histological changes and expression of ECM‐related genes in lamina propria of the vocal fold were evaluated at 4 and 12 weeks after surgery. Results Testosterone and estradiol levels decreased in the ORX and OVX groups, respectively. ORX groups did not have significant changes compared with CON‐ORX groups. However, the expression of hyaluronic acid (HA) was decreased in the OVX group compared with the CON‐OVX group. The expression of collagen I in OVX was lower than in the CON‐OVX group. Collagen III levels were elevated at 4 weeks in the OVX group, but collagen III levels were diminished at 12 weeks in the OVX group. Expression of elastin in the ECM was less dense in the OVX group compared with controls. The expression MMP‐1 and MMP‐9 showed significantly increase in the OVX group compared to the CON‐OVX group. Conclusion No changes of the ECM‐related genes in the vocal fold lamina propria were observed in ORX groups with reduced testosterone. However, changes of several ECM‐related genes were observed in OVX groups with decreased estrogen. These results indicate that the vocal fold is an estrogen‐sensitive target organ and that decreased estrogen, not testosterone, can affect the expression of several ECM‐related molecules of vocal fold. Level of Evidence NA Laryngoscope , 130:732–740, 2020
    Type of Medium: Online Resource
    ISSN: 0023-852X , 1531-4995
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 2026089-1
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  • 7
    In: Advanced Electronic Materials, Wiley, Vol. 7, No. 8 ( 2021-08)
    Abstract: This work demonstrates a facile way to fabricate ultrastretchable thermo‐ and mechanochromic fiber with healable metallic conductivity. Thermochromic hollow elastic fiber is prepared by using silicone composite with thermochromic pigment. Once the fiber forms, gallium‐based liquid metal is injected into the fiber. The composite fiber with liquid metal core shows uniform color change along the fiber by applying Joule heating through the liquid metal wire. The fiber with multiple thermochromic pigment locally distributed along the fiber shows serial color change by increased current. The healable conductivity of the metal core is demonstrated by restoring electrical conductivity near room temperature. When it is in a solid state, the wire can fracture during deformation, thus the wire loses conductivity. However, upon body heating, the wire can rewire, because of the low melting point of the metal (gallium, 29.8 °C). This healing ability of the metal core can allow the fiber to have healable thermochromic behavior. Stretching a liquid metal wire can change the geometry of the wires, resulting in a color change because of increased resistance while Joule heating is applied. This thermo‐ and mechanochromic fiber with liquid metal core would find use in wearable and conformal electronics, electronic textiles, and soft robotics.
    Type of Medium: Online Resource
    ISSN: 2199-160X , 2199-160X
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 2810904-1
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  • 8
    Online Resource
    Online Resource
    Wiley ; 2020
    In:  PM&R Vol. 12, No. 1 ( 2020-01), p. 104-106
    In: PM&R, Wiley, Vol. 12, No. 1 ( 2020-01), p. 104-106
    Type of Medium: Online Resource
    ISSN: 1934-1482 , 1934-1563
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 2480906-8
    SSG: 31
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  • 9
    In: Allergy, Wiley, Vol. 76, No. 1 ( 2021-01), p. 223-232
    Abstract: While the clinical characteristics and outcomes of asthma‐chronic obstructive pulmonary disease (COPD) overlap (ACO) have been frequently compared with those of COPD or asthma, the prevalence and features of ACO in patients with severe asthma are unclear. Objectives Evaluation of the prevalence and clinical features of ACO using the Korean severe asthma registry. Methods At the time of registration, ACO was determined in patients with severe asthma by attending specialists. Patients were classified into ACO and non‐ACO groups, and the demographic and clinical characteristics of these two groups were compared. Results Of 482 patients with severe asthma, 23.7% had ACO. Patients in the ACO group were more likely to be male ( P   〈  .001), older ( P   〈  .001), and ex‐ or current smokers ( P   〈  .001) compared with those in the non‐ACO group. Patients in the ACO group had lower mean forced expiratory volume in 1 second ( P   〈  .001) and blood eosinophil percentage ( P  = .006), but higher blood neutrophil percentage ( P  = .027) than those in the non‐ACO group. The ACO group used more inhaled long‐acting muscarinic antagonist ( P   〈  .001), methylxanthine ( P  = .001), or sustained systemic corticosteroid ( P  = .002). In addition, unscheduled emergency department visits due to exacerbation were more frequent in the ACO group ( P  = .006). Conclusion Among patients with severe asthma, those with ACO were older, predominantly male, and were more likely to have a smoking history than those with asthma only. Patients with ACO used more systemic corticosteroid and had more frequent exacerbations related to emergency department visits than those with severe asthma only.
    Type of Medium: Online Resource
    ISSN: 0105-4538 , 1398-9995
    URL: Issue
    RVK:
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 2003114-2
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  • 10
    In: Annals of Neurology, Wiley, Vol. 93, No. 4 ( 2023-04), p. 768-782
    Abstract: Heritability of stroke is assumed not to be low, especially in the young stroke population. However, most genetic studies have been performed in highly selected patients with typical clinical or neuroimaging characteristics. We investigated the prevalence of 15 Mendelian stroke genes and explored the relationships between variants and the clinical and neuroimaging characteristics in a large, unselected, young stroke population. Methods We enrolled patients aged ≤55 years with stroke or transient ischemic attack from a prospective, nationwide, multicenter stroke registry. We identified clinically relevant genetic variants (CRGVs) in 15 Mendelian stroke genes ( GLA , NOTCH3 , HTRA1 , RNF213 , ACVRL1 , ENG , CBS , TREX1 , ABCC6 , COL4A1 , FBN1 , NF1 , COL3A1 , MT‐TL1 , and APP ) using a customized, targeted next generation sequencing panel. Results Among 1,033 patients, 131 (12.7%) had 28 CRGVs, most frequently in RNF213 (n = 59), followed by ABCC6 ( n  = 53) and NOTCH3 (n = 15). The frequency of CRGVs differed by ischemic stroke subtypes ( p   〈  0.01): the highest in other determined etiology (20.1%), followed by large artery atherosclerosis (13.6%). It also differed between patients aged ≤35 years and those aged 51 to 55 years (17.1% vs 9.3%, p  = 0.02). Only 27.1% and 26.7% of patients with RNF213 and NOTCH3 variants had typical neuroimaging features of the corresponding disorders, respectively. Variants of uncertain significance (VUSs) were found in 15.4% patients. Interpretation CRGVs in 15 Mendelian stroke genes may not be uncommon in the young stroke population. The majority of patients with CRGVs did not have typical features of the corresponding monogenic disorders. Clinical implications of having CRGVs or VUSs should be explored. ANN NEUROL 2023;93:768–782
    Type of Medium: Online Resource
    ISSN: 0364-5134 , 1531-8249
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2037912-2
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