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  • Springer Science and Business Media LLC  (6)
  • 2020-2024  (6)
  • 1
    Online Resource
    Online Resource
    Dietary zinc enrichment reduces the cadmium burden of mealworm beetle (Tenebrio molitor) larvae
    Springer Science and Business Media LLC ; 2020
    In:  Scientific Reports Vol. 10, No. 1 ( 2020-11-18)
    Details
    In: Scientific Reports, Springer Science and Business Media LLC, Vol. 10, No. 1 ( 2020-11-18)
    Abstract: The industrial production of Tenebrio molitor L. requires optimized rearing and processing conditions to generate insect biomass with high nutritional value in large quantities. One of the problems arising from processing is a tremendous loss in mineral accessibility, affecting, amongst others, the essential trace element Zn. As a feasible strategy this study investigates Zn-enrichment of mealworms during rearing to meet the nutritional requirements for humans and animals. Following feeding ZnSO 4 -spiked wheat bran substrates late instar mealworm larvae were evaluated for essential micronutrients and human/animal toxic elements. In addition, growth rate and viability were assessed to select optimal conditions for future mass-rearing. Zn-feeding dose-dependently raised the total Zn content, yet the Zn larvae /Zn wheat bran ratio decreased inversely related to its concentration, indicating an active Zn homeostasis within the mealworms. The Cu status remained stable, suggesting that, in contrast to mammals, the intestinal Cu absorption in mealworm larvae is not affected by Zn. Zn biofortification led to a moderate Fe and Mn reduction in mealworms, a problem that certainly can be overcome by Fe/Mn co-supplementation during rearing. Most importantly, Zn feeding massively reduced the levels of the human/animal toxicant Cd within the mealworm larvae, a technological novelty of outstanding importance to be implemented in the future production process to ensure the consumer safety of this edible insect species.
    Type of Medium: Online Resource
    ISSN: 2045-2322
    URL: Article
    DOI: 10.1038/s41598-020-77079-x
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2020
    detail.hit.zdb_id: 2615211-3
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  • 2
    Online Resource
    Online Resource
    Comparison of Free Zinc Levels Determined by Fluorescent Probes in THP1 Cells Using Microplate Reader and Flow Cytometer
    Springer Science and Business Media LLC ; 2021
    In:  Biological Trace Element Research Vol. 199, No. 6 ( 2021-06), p. 2414-2419
    Details
    In: Biological Trace Element Research, Springer Science and Business Media LLC, Vol. 199, No. 6 ( 2021-06), p. 2414-2419
    Abstract: Free zinc is involved in signal transduction within mammalian cells, acting as a second messenger. Gold standard for its analysis is currently the use of metal-responsive fluorescent probes. The present study elucidates the impact of instrumentation used for measuring the resulting fluorescence. The free zinc concentration of THP-1 cells loaded with the fluorescent probes Zinpyr-1 (ZP1) or Fluozin-3 AM (FZ3) was determined using a microplate reader (MPR) and a flow cytometer (FC). Depending on the instrumentation, either low nanomolar (MPR) or picomolar (FC) concentrations of free zinc were observed. The concentrations measured from identical samples by MPR were about 40 (ZP1) or 165 (FZ3) times higher compared with FC. These results demonstrate that the choice of instrumentation has a fundamental impact on the determination of intracellular free zinc concentrations by low molecular weight fluorescent probes.
    Type of Medium: Online Resource
    ISSN: 0163-4984 , 1559-0720
    URL: Article
    DOI: 10.1007/s12011-020-02355-w
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2021
    detail.hit.zdb_id: 2072581-4
    SSG: 12
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  • 3
    Online Resource
    Online Resource
    Effects of combined cannabidiol (CBD) and hops (Humulus lupulus) terpene extract treatment on RAW 264.7 macrophage viability and inflammatory markers
    Springer Science and Business Media LLC ; 2023
    In:  Natural Products and Bioprospecting Vol. 13, No. 1 ( 2023-12)
    Details
    In: Natural Products and Bioprospecting, Springer Science and Business Media LLC, Vol. 13, No. 1 ( 2023-12)
    Abstract: This study investigates the potential of cannabidiol (CBD), one major cannabinoid of the plant Cannabis sativa , alone and in combination with a terpene-enriched extract from Humulus lupulus (“Hops 1”), on the LPS-response of RAW 264.7 macrophages as an established in vitro model of inflammation. With the present study, we could support earlier findings of the anti-inflammatory potential of CBD, which showed a dose-dependent [0–5 µM] reduction in nitric oxide and tumor necrosis factor-alpha (TNF-α) released by LPS-stimulated RAW 264.7 macrophages. Moreover, we observed an additive anti-inflammatory effect after combined CBD [5 µM] and hops extract [40 µg/mL] treatment. The combination of CBD and Hops 1 showed effects in LPS-stimulated RAW 264.7 cells superior to the single substance treatments and akin to the control hydrocortisone. Furthermore, cellular CBD uptake increased dose-dependently in the presence of terpenes from Hops 1 extract. The anti-inflammatory effect of CBD and its cellular uptake positively correlated with terpene concentration, as indicated by comparison with a hemp extract containing both CBD and terpenes. These findings may contribute to the postulations for the so-called “entourage effect” between cannabinoids and terpenes and support the potential of CBD combined with phytomolecules from a non-cannabinoid source, such as hops, for the treatment of inflammatory diseases.
    Type of Medium: Online Resource
    ISSN: 2192-2195 , 2192-2209
    URL: Article
    DOI: 10.1007/s13659-023-00382-3
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2023
    detail.hit.zdb_id: 2636293-4
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  • 4
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    Online Resource
    Trace element profile and incidence of type 2 diabetes, cardiovascular disease and colorectal cancer: results from the EPIC-Potsdam cohort study
    Springer Science and Business Media LLC ; 2021
    In:  European Journal of Nutrition Vol. 60, No. 6 ( 2021-09), p. 3267-3278
    Details
    In: European Journal of Nutrition, Springer Science and Business Media LLC, Vol. 60, No. 6 ( 2021-09), p. 3267-3278
    Abstract: We aimed to examine the prospective association between manganese, iron, copper, zinc, iodine, selenium, selenoprotein P, free zinc, and their interplay, with incident type 2 diabetes (T2D), cardiovascular disease (CVD) and colorectal cancer (CRC). Methods Serum trace element (TE) concentrations were measured in a case-cohort study embedded within the EPIC-Potsdam cohort, consisting of a random sub-cohort ( n  = 2500) and incident cases of T2D ( n  = 705), CVD ( n  = 414), and CRC ( n  = 219). TE patterns were investigated using principal component analysis. Cox proportional hazard models were fitted to examine the association between TEs with T2D, CVD and CRC incidence. Results Higher manganese, zinc, iodine and selenium were associated with an increased risk of developing T2D (HR Q5 vs Q1: 1.56, 1.09–2.22; HR per SD, 95% CI 1.18, 1.05–1.33; 1.09, 1.01–1.17; 1.19, 1.06–1.34, respectively). Regarding CVD, manganese, copper and copper-to-zinc ratio were associated with an increased risk (HR per SD, 95% CI 1.13, 1.00–1.29; 1.22, 1.02–1.44; 1.18, 1.02–1.37, respectively). The opposite was observed for higher selenium-to-copper ratio (HR Q5 vs Q1, 95% CI 0.60, 0.39–0.93). Higher copper and zinc were associated with increasing risk of developing CRC (HR per SD, 95% CI 1.29, 1.05–1.59 and 1.14, 1.00–1.30, respectively). Selenium, selenoprotein P and selenium-to-copper-ratio were associated to decreased risk (HR per SD, 95% CI 0.82, 0.69–0.98; 0.81, 0.72–0.93; 0.77, 0.65–0.92, respectively). Two TE patterns were identified: manganese–iron–zinc and copper–iodine–selenium. Conclusion Different TEs were associated with the risk of developing T2D, CVD and CRC. The contrasting associations found for selenium with T2D and CRC point towards differential disease-related pathways.
    Type of Medium: Online Resource
    ISSN: 1436-6207 , 1436-6215
    URL: Article
    DOI: 10.1007/s00394-021-02494-3
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2021
    detail.hit.zdb_id: 1463312-7
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  • 5
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    Online Resource
    Excessive copper impairs intrahepatocyte trafficking and secretion of selenoprotein P
    Springer Science and Business Media LLC ; 2023
    In:  Nature Communications Vol. 14, No. 1 ( 2023-06-13)
    Details
    In: Nature Communications, Springer Science and Business Media LLC, Vol. 14, No. 1 ( 2023-06-13)
    Abstract: Selenium homeostasis depends on hepatic biosynthesis of selenoprotein P (SELENOP) and SELENOP-mediated transport from the liver to e.g. the brain. In addition, the liver maintains copper homeostasis. Selenium and copper metabolism are inversely regulated, as increasing copper and decreasing selenium levels are observed in blood during aging and inflammation. Here we show that copper treatment increased intracellular selenium and SELENOP in hepatocytes and decreased extracellular SELENOP levels. Hepatic accumulation of copper is a characteristic of Wilson’s disease. Accordingly, SELENOP levels were low in serum of Wilson’s disease patients and Wilson’s rats. Mechanistically, drugs targeting protein transport in the Golgi complex mimicked some of the effects observed, indicating a disrupting effect of excessive copper on intracellular SELENOP transport resulting in its accumulation in the late Golgi. Our data suggest that hepatic copper levels determine SELENOP release from the liver and may affect selenium transport to peripheral organs such as the brain.
    Type of Medium: Online Resource
    ISSN: 2041-1723
    URL: Article
    DOI: 10.1038/s41467-023-39245-3
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2023
    detail.hit.zdb_id: 2553671-0
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  • 6
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    Online Resource
    A fluorometric assay to determine labile copper(II) ions in serum
    Springer Science and Business Media LLC ; 2023
    In:  Scientific Reports Vol. 13, No. 1 ( 2023-08-07)
    Details
    In: Scientific Reports, Springer Science and Business Media LLC, Vol. 13, No. 1 ( 2023-08-07)
    Abstract: Labile copper(II) ions (Cu 2+ ) in serum are considered to be readily available for cellular uptake and to constitute the biologically active Cu 2+ species in the blood. It might also be suitable to reflect copper dyshomeostasis during diseases such as Wilson’s disease (WD) or neurological disorders. So far, no direct quantification method has been described to determine this small Cu 2+ subset. This study introduces a fluorometric high throughput assay using the novel Cu 2+ binding fluoresceine-peptide sensor FP4 (Kd of the Cu 2+ -FP4-complex 0.38 pM) to determine labile Cu 2+ in human and rat serum. Using 96 human serum samples, labile Cu 2+ was measured to be 0.14 ± 0.05 pM, showing no correlation with age or other serum trace elements. No sex-specific differences in labile Cu 2+ concentrations were noted, in contrast to the total copper levels in serum. Analysis of the effect of drug therapy on labile Cu 2+ in the sera of 19 patients with WD showed a significant decrease in labile Cu 2+ following copper chelation therapy, suggesting that labile Cu 2+ may be a specific marker of disease status and that the assay could be suitable for monitoring treatment progress.
    Type of Medium: Online Resource
    ISSN: 2045-2322
    URL: Article
    DOI: 10.1038/s41598-023-39841-9
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2023
    detail.hit.zdb_id: 2615211-3
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