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  • SAGE Publications  (28)
  • 2020-2024  (28)
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  • SAGE Publications  (28)
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  • 2020-2024  (28)
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  • 1
    Online Resource
    Online Resource
    Fibulin-1 Regulates Initiation of Successional Dental Lamina
    SAGE Publications ; 2023
    In:  Journal of Dental Research Vol. 102, No. 11 ( 2023-10), p. 1220-1230
    Details
    In: Journal of Dental Research, SAGE Publications, Vol. 102, No. 11 ( 2023-10), p. 1220-1230
    Abstract: In humans, teeth are replaced only once, and the successional dental lamina (SDL) of the permanent tooth is maintained in a quiescent state until adolescence. Recently, we showed that biomechanical stress generated by the rapid growth of the deciduous tooth inhibits SDL development via integrin β1–RUNX2 signaling at embryonic day 60 (E60) in miniature pigs. However, the mechanism by which RUNX2 regulates SDL initiation within the SDL stem cell niche remains unclear. In the current study, we transcriptionally profiled single cells from SDL and surrounding mesenchyme at E60 and identified the landscape of cellular heterogeneity. We then identified a specific fibroblast subtype in the dental follicle mesenchyme between the deciduous tooth and the SDL of the permanent tooth (DFDP), which constitutes the inner part of the niche (deciduous tooth side). Compared with traditional dental follicle cells, the specific expression profile of DFDP was identified and found to be related to biomechanical stress. Subsequently, we found that RUNX2 could bind to the enhancer regions of Fbln1 (gene of fibulin-1), one of the marker genes for DFDP. Through gain- and loss-of-function experiments, we proved that the biomechanical stress–mediated RUNX2–fibulin-1 axis inhibits the initiation of SDL by maintaining SDL niche homeostasis.
    Type of Medium: Online Resource
    ISSN: 0022-0345 , 1544-0591
    URL: Article
    DOI: 10.1177/00220345231182052
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2023
    detail.hit.zdb_id: 2057074-0
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  • 2
    Online Resource
    Online Resource
    Apical Papilla Regulates Dental Follicle Fate via the OGN-Hh Pathway
    SAGE Publications ; 2023
    In:  Journal of Dental Research Vol. 102, No. 4 ( 2023-04), p. 431-439
    Details
    In: Journal of Dental Research, SAGE Publications, Vol. 102, No. 4 ( 2023-04), p. 431-439
    Abstract: Root apical complex, including Hertwig’s epithelial root sheath, apical papilla, and dental follicle (DF), is the germinal center of root development, wherein the DF constantly develops into periodontal tissue. However, whether DF development is regulated by the adjacent apical papilla remains largely unknown. In this study, we employed a transwell coculture system and found that stem cells from the apical papilla (SCAPs) inhibit the differentiation and maintain the stemness of dental follicle stem cells (DFSCs). Meanwhile, partial SCAP differentiation markers were upregulated after DFSC coculture. High-throughput RNA sequencing revealed that the Hedgehog (Hh) pathway was significantly downregulated in DFSCs cocultured with SCAPs. Upregulation or downregulation of the Hh pathway can respectively activate or inhibit the multidirectional differentiation of DFSCs. Osteoglycin (OGN) (previously known as mimecan) is highly expressed in the dental papilla, similarly to Hh pathway factors. By secreting OGN, SCAP regulated the stemness and multidirectional differentiation of DFSCs via the OGN-Hh pathway. Finally, Ogn –/– mice were established using the CRISPR/Cas9 system. We found that the root length growth rate was accelerated during root development from PN0 to PN30 in Ogn –/– mice. Moreover, the hard tissues (including dentin and cementum) of the root in Ogn –/– mice were thicker than those in wild-type mice. These phenotypes were likely due to Hh pathway activation and the increased cell proliferation and differentiation in both the apical papilla and DF. The current work elucidates the molecular regulation of early periodontal tissue development, providing a theoretical basis for future research on tooth root biology and periodontal tissue regeneration.
    Type of Medium: Online Resource
    ISSN: 0022-0345 , 1544-0591
    URL: Article
    DOI: 10.1177/00220345221138517
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2023
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  • 3
    Online Resource
    Online Resource
    CBD Promotes Oral Ulcer Healing via Inhibiting CMPK2-Mediated Inflammasome
    SAGE Publications ; 2022
    In:  Journal of Dental Research Vol. 101, No. 2 ( 2022-02), p. 206-215
    Details
    In: Journal of Dental Research, SAGE Publications, Vol. 101, No. 2 ( 2022-02), p. 206-215
    Abstract: Oral ulcer is a common oral inflammatory lesion accompanied by severe pain but with few effective treatments. Cannabidiol (CBD) is recently emerging for its therapeutic potential in a range of diseases, including inflammatory conditions and cancers. Here we show that CBD oral spray on acid- or trauma-induced oral ulcers on mice tongue inhibits inflammation, relieves pain, and accelerates lesion closure. Notably, the enrichment of genes associated with the NOD, LRR, and NLRP3 pyrin domain–containing protein 3 (NLRP3) inflammasome pathway is downregulated after CBD treatment. The expression of cleaved-gasdermin D (GSDMD) and the percentage of pyroptotic cells are reduced as well. In addition, CBD decreases the expression of cytidine/uridine monophosphate kinase 2 (CMPK2), which subsequently inhibits the generation of oxidized mitochondria DNA and suppresses inflammasome activation. These immunomodulating effects of CBD are mostly blocked by peroxisome proliferator activated receptor γ (PPARγ) antagonist and partially antagonized by CB 1 receptor antagonist. Our results demonstrate that CBD accelerates oral ulcer healing by inhibiting CMPK2-mediated NLRP3 inflammasome activation and pyroptosis, which are mediated mostly by PPARγ in the nucleus and partially by CB 1 in the plasma membrane.
    Type of Medium: Online Resource
    ISSN: 0022-0345 , 1544-0591
    URL: Article
    DOI: 10.1177/00220345211024528
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2022
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  • 4
    Online Resource
    Online Resource
    Single-Cell Transcriptomic Atlas of Gingival Mucosa in Type 2 Diabetes
    SAGE Publications ; 2022
    In:  Journal of Dental Research Vol. 101, No. 13 ( 2022-12), p. 1654-1664
    Details
    In: Journal of Dental Research, SAGE Publications, Vol. 101, No. 13 ( 2022-12), p. 1654-1664
    Abstract: The oral gingival barrier is a constantly stimulated and dynamic environment where homeostasis is often disrupted, resulting in inflammatory periodontal diseases. Type 2 diabetes (T2D) has been reported to be associated with gingival barrier dysfunction, but the effect and underlying mechanism are inconclusive. Herein, we performed single-cell RNA sequencing (scRNA-seq) of gingiva from leptin receptor-deficient mice ( db/db) to examine the gingival heterogeneity in the context of T2D. Periodontal health of control mice is characterized by populations of Krt14 + -expressing epithelial cells and Col1a1 + -fibroblasts mediating immune homeostasis primarily through the enrichment of innate lymphoid cells. The db/db gingiva exhibited decreased epithelial/stromal ratio and dysfunctional barrier. We further observed stromal, particularly fibroblast immune hyperresponsiveness, linked to the recruitment of myeloid-derived cells at the db/db gingiva. Both scRNA-seq and histological analysis suggested the inflammatory signaling between fibroblasts and neutrophils as a potential driver of diabetes-induced periodontal damage. Notably, the “immune-like” stromal cells were wired toward the induction of gingival IL-17A hyperresponsiveness in db/db mice. Our work reveals that the “immune-like” fibroblasts with transcriptional diversity are involved in the innate immune homeostasis at the diabetic gingiva. It highlights a potentially significant role of these cell types in its pathogenesis.
    Type of Medium: Online Resource
    ISSN: 0022-0345 , 1544-0591
    URL: Article
    DOI: 10.1177/00220345221092752
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2022
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  • 5
    Online Resource
    Online Resource
    Multilevel Pathways of Colorectal Cancer Screening Among Low-Income Vietnamese Americans: A Structural Equation Modeling Analysis
    SAGE Publications ; 2021
    In:  Cancer Control Vol. 28 ( 2021-01-01), p. 107327482110110-
    Details
    In: Cancer Control, SAGE Publications, Vol. 28 ( 2021-01-01), p. 107327482110110-
    Abstract: Colorectal cancer (CRC) disproportionately affects Vietnamese Americans, especially those with low income and were born outside of the United States. CRC screening tests are crucial for prevention and early detection. Despite the availability of noninvasive, simple-to-conduct tests, CRC screening rates in Asian Americans, particularly Vietnamese Americans, remain suboptimal. The purpose of this study was to evaluate the interplay of multilevel factors – individual, interpersonal, and community – on CRC screening behaviors among low-income Vietnamese Americans with limited English proficiency. Methods: This study is based on the Sociocultural Health Behavior Model, a research-based model that incorporates 6 factors associated with decision-making and health-seeking behaviors that result in health care utilization. Using a community-based participatory research approach, we recruited 801 Vietnamese Americans from community-based organizations. We administered a survey to collect information on sociodemographic characteristics, health-related factors, and CRC screening-related factors. We used structural equation modeling (SEM) to identify direct and indirect predictors of lifetime CRC screening. Results: Bivariate analysis revealed that a greater number of respondents who never screened for CRC reported limited English proficiency, fewer years of US residency, and lower self-efficacy related to CRC screening. The SEM model identified self-efficacy (coefficient = 0.092, P 〈 .01) as the only direct predictor of lifetime CRC screening. Educational attainment (coefficient = 0.13, P 〈 .01) and health beliefs (coefficient = 0.040, P 〈 .001) had a modest significant positive relationship with self-efficacy. Health beliefs (coefficient = 0.13, P 〈 .001) and educational attainment (coefficient = 0.16, P 〈 .01) had significant positive relationships with CRC knowledge. Conclusions: To increase CRC screening uptake in medically underserved Vietnamese American populations, public health interventions should aim to increase community members’ confidence in their abilities to screen for CRC and to navigate associated processes, including screening preparation, discussions with doctors, and emotional complications.
    Type of Medium: Online Resource
    ISSN: 1073-2748 , 1073-2748
    URL: Article
    DOI: 10.1177/10732748211011077
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2021
    detail.hit.zdb_id: 2004182-2
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  • 6
    Online Resource
    Online Resource
    Empowering Low-Income Asian American Women to Conduct Human Papillomavirus Self-Sampling Test: A Community-Engaged and Culturally Tailored Intervention
    SAGE Publications ; 2022
    In:  Cancer Control Vol. 29 ( 2022-01), p. 107327482210768-
    Details
    In: Cancer Control, SAGE Publications, Vol. 29 ( 2022-01), p. 107327482210768-
    Abstract: Asian American women face disproportionate burden of cervical cancer (CC) than non-Hispanic white women in the U.S. The goal of this study was to assess the feasibility and impact of a culturally tailored intervention to promote Human papillomavirus (HPV) self-sampling test among hard-to-reach Asian American women. Methods We adopted the community-based participatory research (CBPR) approach to conduct this efficacy study. A total of 156 female participants (56 Chinese, 50 Korean, and 50 Vietnamese) were recruited from community-based organizations (CBOs) in the greater Philadelphia metropolitan area. The intervention components included HPV-related education, HPV self-sampling test kit and instructions, group discussions, and patient navigations, all available in Asian languages. We examined several outcomes, including the completion of HPV self-sampling, HPV-related knowledge, perceived social support, self-efficacy, and comfort with the self-sampling test at post-intervention assessment. Results The majority of Asian American women had low annual household income (62.3% earned less than $20,000) and low educational attainment (61.3% without a college degree). We found significant increase in participants’ knowledge on HPV (baseline: 2.83, post: 4.89, P 〈 .001), social support (baseline: 3.91, post: 4.09, P 〈 .001), self-efficacy (baseline: 3.05, post: 3.59, P 〈 .001), and comfortable with HPV self-sample test (baseline: 3.62, post: 4.06, P 〈 .001). Conclusion To the best of our knowledge, this is the first intervention study that promoted HPV self-sampling test among Asian American women. Our findings showed that CBPR culturally tailored intervention of self-sampling was highly effective in empowering low-income Asian American women to conduct HPV self-sampling tests.
    Type of Medium: Online Resource
    ISSN: 1073-2748 , 1526-2359
    URL: Article
    DOI: 10.1177/10732748221076813
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2022
    detail.hit.zdb_id: 2004182-2
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  • 7
    Online Resource
    Online Resource
    Developing Academics’ Capacity for Internationalizing the Curriculum: A Collaborative Autoethnography of a Cross-Institutional Project
    SAGE Publications ; 2022
    In:  Journal of Studies in International Education Vol. 26, No. 3 ( 2022-07), p. 334-351
    Details
    In: Journal of Studies in International Education, SAGE Publications, Vol. 26, No. 3 ( 2022-07), p. 334-351
    Abstract: Developing academics’ capacity for internationalizing the curriculum (IoC) is essential but challenging. There is a lack of understanding of how the IoC framework can be implemented in reality and of how educational developers can facilitate the process. This collaborative autoethnography explores the cultivation over 3 years of a community of practice for developing academics’ IoC capacity facilitated by six educational developers in three universities in Hong Kong. The findings reveal a complex balancing act among various forces in the areas of domain, community, practice, power, identity, and broader context, the last three of which are insufficiently addressed in the literature. This article adds value by illustrating how IoC theories and communities of practice interact with multiple forces in the environment and our own agency, and are negotiated within a particular context.
    Type of Medium: Online Resource
    ISSN: 1028-3153 , 1552-7808
    URL: Article
    DOI: 10.1177/1028315320976040
    RVK:
    AL 17900
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2022
    detail.hit.zdb_id: 2075289-1
    SSG: 5,3
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  • 8
    Online Resource
    Online Resource
    Ulinastatin alleviates mitochondrial damage and cell apoptosis induced by isoflurane in human neuroglioma H4 cells
    SAGE Publications ; 2020
    In:  Human & Experimental Toxicology Vol. 39, No. 10 ( 2020-10), p. 1417-1425
    Details
    In: Human & Experimental Toxicology, SAGE Publications, Vol. 39, No. 10 ( 2020-10), p. 1417-1425
    Abstract: Isoflurane has been demonstrated to induce mitochondrial damage and cell apoptosis. The isoflurane-induced inflammation may be an important reason for this phenomenon. Studies have shown that ulinastatin (UTI) has an anti-inflammatory effect. Our aim was to investigate whether UTI could attenuate isoflurane-induced mitochondrial damage and cell apoptosis by inhibiting inflammation. Human neuroglioma H4 cells were exposed to isoflurane with or without UTI. The ratio of cell apoptosis was evaluated by flow cytometry. β-Amyloid (Aβ) peptide and cleaved caspase 3 expression were evaluated by Western blot analysis. The concentrations of tumor necrosis factor α (TNF-α) and interleukin 1β (IL-1β) were detected by sandwich enzyme-linked immunosorbent assays. Mitochondrial structural changes were detected by transmission electron microscopy. Mitochondrial membrane potential (Δψm) was determined by 5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethyl-imidacarbocyanine iodide (JC-1). The activity of the mitochondrial electron transport chain (ETC) complexes I, II, III, and IV was determined by assay kits. UTI attenuated the TNF-α and IL-1β release induced by isoflurane. UTI could also reduce mitochondrial structure damage, mitigate the decrease in Δψm, and improve ETC complexes dysfunction. Furthermore, it decreased cell apoptosis induced by isoflurane in H4 cells. UTI had no effect on isoflurane-induced Aβ expression. UTI may mitigate isoflurane-induced mitochondrial damage and cytotoxicity by inhibiting inflammation.
    Type of Medium: Online Resource
    ISSN: 0960-3271 , 1477-0903
    URL: Article
    DOI: 10.1177/0960327120926242
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2020
    detail.hit.zdb_id: 1483723-7
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  • 9
    Online Resource
    Online Resource
    (Z)-7,4′-Dimethoxy-6-hydroxy-aurone- 4-O-β-glucopyranoside alleviates cerebral ischemia-reperfusion injury in rats associating with the regulation of JAK1/STAT1 signaling pathway
    SAGE Publications ; 2020
    In:  Human & Experimental Toxicology Vol. 39, No. 11 ( 2020-11), p. 1507-1517
    Details
    In: Human & Experimental Toxicology, SAGE Publications, Vol. 39, No. 11 ( 2020-11), p. 1507-1517
    Abstract: Inflammatory responses have been demonstrated to contribute to the neuronal death following cerebral ischemia. This study was to investigate the repairing effects and potential mechanisms of (Z)-7,4′-dimethoxy-6-hydroxy-aurone-4-O-β-glucopyranoside (DHAG), a compound with neuroprotective effects, on cerebral ischemia-reperfusion (I/R) injury in rats. Cerebral I/R model was established with middle cerebral artery occlusion method in Sprague Dawley rats and then rats were treated with DHAG (1 and 2 mg/kg) for 7 days. The volume of cerebral infarction was detected by triphenyltetrazolium chloride staining. The apoptosis in ischemic brain tissues was detected by terminal deoxynucleotidyl transferase dUTP nick end labeling assay. Oxidative stress markers and inflammatory factors were detected by enzyme-linked immunosorbent assay. Protein expression was detected by Western blot. DHAG treatment significantly alleviated the cerebral I/R injury and decreased apoptosis in brain tissues. Moreover, DHAG treatment significantly inhibited oxidative stress and reduced inflammatory responses, associating with decreasing the protein expression of phosphorylated Janus kinase 1/phosphorylated signal transducer and transcriptional activator 1. These results demonstrated neuroprotective properties of DHAG and highlighted it as a potential therapeutic agent against injury of cerebral IR.
    Type of Medium: Online Resource
    ISSN: 0960-3271 , 1477-0903
    URL: Article
    DOI: 10.1177/0960327120927439
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2020
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  • 10
    Online Resource
    Online Resource
    Activated Epithelial FGF8 Signaling Induces Fused Supernumerary Incisors
    SAGE Publications ; 2022
    In:  Journal of Dental Research Vol. 101, No. 4 ( 2022-04), p. 458-464
    Details
    In: Journal of Dental Research, SAGE Publications, Vol. 101, No. 4 ( 2022-04), p. 458-464
    Abstract: FGF8, which is specifically expressed in the dental epithelium prior to the E12.5 bud stage, is a key player during odontogenesis, being responsible for the initiation of tooth development. Here, to investigate the impact of persistent FGF8 signaling on tooth development, we forcibly activated FGF8 signaling in the dental epithelium after the bud stage by generating K14-Cre;R26R-Fg8 mice. We found that a unique type of fused supernumerary incisors is formed, although morphologically resembling the features of type II dens invaginatus in humans. Further analysis revealed that ectopically activated epithelial FGF8 alters the cell fate of the incisor lingual outer enamel epithelium, endowing it with odontogenic potential by the activation of several key tooth genes, including Pitx2, Sox2, Lef-1, p38, and Erk1/2, and induces de novo formation of an extra incisor crown lingually in parallel to the original one, leading to the formation of an extra incisor crown and fused with the original incisor eventually. Meanwhile, the overdosed epithelial FGF8 signaling dramatically downregulates the expression of mesenchymal Bmp4, leading to severely impaired enamel mineralization. Based on the location of the extra incisors, we propose that they are likely to be rescued replacement teeth. Our results further demonstrate the essential role of FGF8 signaling for tooth initiation and the establishment of progenitor cells of dental epithelial stem cells during development.
    Type of Medium: Online Resource
    ISSN: 0022-0345 , 1544-0591
    URL: Article
    DOI: 10.1177/00220345211046590
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2022
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