In:
PLOS Neglected Tropical Diseases, Public Library of Science (PLoS), Vol. 16, No. 5 ( 2022-5-31), p. e0010435-
Abstract:
Almost all Echinococcus multilocularis ( Em ) infections occur in the liver of the intermediate host, causing a lethal zoonotic helminthic disease, alveolar echinococcosis (AE). However, the long non-coding RNAs (lncRNAs) expression profiles of the host and the potential regulatory function of lncRNA during Em infection are poorly understood. In this study, the profiles of lncRNAs and mRNAs in the liver of mice at different time points after Em infection were explored by microarray. Thirty-one differentially expressed mRNAs (DEMs) and 68 differentially expressed lncRNAs (DELs) were found continuously dysregulated. These DEMs were notably enriched in “antigen processing and presentation”, “Th1 and Th2 cell differentiation” and “Th17 cell differentiation” pathways. The potential predicted function of DELs revealed that most DELs might influence Th17 cell differentiation and TGF-β/Smad pathway of host by trans -regulating SMAD3, STAT1, and early growth response (EGR) genes. At 30 days post-infection (dpi), up-regulated DEMs were enriched in Toll-like and RIG-I-like receptor signaling pathways, which were validated by qRT-PCR, Western blotting and downstream cytokines detection. Furthermore, flow cytometric analysis and serum levels of the corresponding cytokines confirmed the changes in cell-mediated immunity in host during Em infection that showed Th1 and Th17-type CD4 + T-cells were predominant at the early infection stage whereas Th2-type CD4 + T-cells were significantly higher at the middle/late stage. Collectively, our study revealed the potential regulatory functions of lncRNAs in modulating host Th cell subsets and provide novel clues in understanding the influence of Em infection on host innate and adaptive immune response.
Type of Medium:
Online Resource
ISSN:
1935-2735
DOI:
10.1371/journal.pntd.0010435
DOI:
10.1371/journal.pntd.0010435.g001
DOI:
10.1371/journal.pntd.0010435.g002
DOI:
10.1371/journal.pntd.0010435.g003
DOI:
10.1371/journal.pntd.0010435.g004
DOI:
10.1371/journal.pntd.0010435.g005
DOI:
10.1371/journal.pntd.0010435.g006
DOI:
10.1371/journal.pntd.0010435.g007
DOI:
10.1371/journal.pntd.0010435.g008
DOI:
10.1371/journal.pntd.0010435.g009
DOI:
10.1371/journal.pntd.0010435.t001
DOI:
10.1371/journal.pntd.0010435.s001
DOI:
10.1371/journal.pntd.0010435.s002
DOI:
10.1371/journal.pntd.0010435.s003
DOI:
10.1371/journal.pntd.0010435.s004
DOI:
10.1371/journal.pntd.0010435.s005
DOI:
10.1371/journal.pntd.0010435.s006
DOI:
10.1371/journal.pntd.0010435.s007
DOI:
10.1371/journal.pntd.0010435.s008
DOI:
10.1371/journal.pntd.0010435.s009
DOI:
10.1371/journal.pntd.0010435.s010
DOI:
10.1371/journal.pntd.0010435.s011
DOI:
10.1371/journal.pntd.0010435.s012
DOI:
10.1371/journal.pntd.0010435.s013
DOI:
10.1371/journal.pntd.0010435.s014
DOI:
10.1371/journal.pntd.0010435.s015
DOI:
10.1371/journal.pntd.0010435.s016
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2429704-5
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