In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 12 ( 2021-12-31), p. e0262159-
Abstract:
GENECUBE ® is a rapid molecular identification system, and previous studies demonstrated that GENECUBE ® HQ SARS-CoV-2 showed excellent analytical performance for the detection of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) with nasopharyngeal samples. However, other respiratory samples have not been evaluated. Methods This prospective comparison between GENECUBE ® HQ SARS-CoV-2 and reference real-time reverse transcriptase polymerase chain reaction (RT-PCR) was performed for the detection of SARS-CoV-2 using anterior nasal samples and saliva samples. Additionally, we evaluated a new rapid examination protocol using GENECUBE ® HQ SARS-CoV-2 for the detection of SARS-CoV-2 with saliva samples. For the rapid protocol, in the preparation of saliva samples, purification and extraction processes were adjusted, and the total process time was shortened to approximately 35 minutes. Results For 359 anterior nasal samples, the total-, positive-, and negative concordance of the two assays was 99.7% (358/359), 98.1% (51/52), and 100% (307/307), respectively. For saliva samples, the total-, positive-, and negative concordance of the two assays was 99.6% (239/240), 100% (56/56), and 99.5% (183/184), respectively. With the new protocol, total-, positive-, and negative concordance of the two assays was 98.8% (237/240), 100% (56/56), and 98.4% (181/184), respectively. In all discordance cases, SARS-CoV-2 was detected by additional molecular examinations. Conclusion GENECUBE ® HQ SARS-CoV-2 provided high analytical performance for the detection of SARS-CoV-2 in anterior nasal samples and saliva samples.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0262159
DOI:
10.1371/journal.pone.0262159.g001
DOI:
10.1371/journal.pone.0262159.t001
DOI:
10.1371/journal.pone.0262159.t002
DOI:
10.1371/journal.pone.0262159.t003
DOI:
10.1371/journal.pone.0262159.t004
DOI:
10.1371/journal.pone.0262159.t005
DOI:
10.1371/journal.pone.0262159.t006
DOI:
10.1371/journal.pone.0262159.t007
DOI:
10.1371/journal.pone.0262159.t008
DOI:
10.1371/journal.pone.0262159.s001
DOI:
10.1371/journal.pone.0262159.s002
DOI:
10.1371/journal.pone.0262159.s003
DOI:
10.1371/journal.pone.0262159.s004
DOI:
10.1371/journal.pone.0262159.s005
DOI:
10.1371/journal.pone.0262159.s006
DOI:
10.1371/journal.pone.0262159.r001
DOI:
10.1371/journal.pone.0262159.r002
DOI:
10.1371/journal.pone.0262159.r003
DOI:
10.1371/journal.pone.0262159.r004
DOI:
10.1371/journal.pone.0262159.r005
DOI:
10.1371/journal.pone.0262159.r006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2267670-3
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