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  • 1
    In: Fishes, MDPI AG, Vol. 8, No. 2 ( 2023-02-18), p. 116-
    Abstract: The melanocortin-4 receptor (MC4R) plays a critical role in homeostasis and the regulation of body weight. Polymorphisms in the mc4r gene have been discovered and linked to growth, carcass composition, and meat quality traits. Therefore, we used the CRISPR-Cas9 system to target the mc4r gene in the most important freshwater aquaculture species in the USA, channel catfish, Ictalurus punctatus. Guide RNAs were designed to direct the Cas9 to the coding sequence of the channel catfish mc4r gene. gRNA(s)-Cas9 mixtures were delivered into one-cell embryos using electroporation and microinjection. For each treatment, the nature and rate of mutations were analyzed. Hatching and survival rates were calculated. The overall mutation rates were 30.6% and 66.7–90.6% for electroporation and microinjection, respectively. Mutated fish generated via electroporation or microinjection exhibited 38% and 20% improvement in body weight, respectively, when compared with the full-sib control. The mean feed conversion ratio of the mutants was 1.18 compared with 1.57 in the control fish. The improved growth and feed conversion indicate that the generation of mc4r-edited fish could economically benefit aquaculture production.
    Type of Medium: Online Resource
    ISSN: 2410-3888
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2932929-2
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  • 2
    Online Resource
    Online Resource
    MDPI AG ; 2022
    In:  Electronics Vol. 11, No. 23 ( 2022-11-28), p. 3929-
    In: Electronics, MDPI AG, Vol. 11, No. 23 ( 2022-11-28), p. 3929-
    Abstract: Handling missing values (MVs) and feature selection (FS) are vital preprocessing tasks for many pattern recognition, data mining, and machine learning (ML) applications, involving classification and regression problems. The existence of MVs in data badly affects making decisions. Hence, MVs have to be taken into consideration during preprocessing tasks as a critical problem. To this end, the authors proposed a new algorithm for manipulating MVs using FS. Bayesian ridge regression (BRR) is the most beneficial type of Bayesian regression. BRR estimates a probabilistic model of the regression problem. The proposed algorithm is dubbed as cumulative Bayesian ridge with similarity and Luca’s fuzzy entropy measure (CBRSL). CBRSL reveals how the fuzzy entropy FS used for selecting the candidate feature holding MVs aids in the prediction of the MVs within the selected feature using the Bayesian Ridge technique. CBRSL can be utilized to manipulate MVs within other features in a cumulative order; the filled features are incorporated within the BRR equation in order to predict the MVs for the next selected incomplete feature. An experimental analysis was conducted on four datasets holding MVs generated from three missingness mechanisms to compare CBRSL with state-of-the-art practical imputation methods. The performance was measured in terms of R2 score (determination coefficient), RMSE (root mean square error), and MAE (mean absolute error). Experimental results indicate that the accuracy and execution times differ depending on the amount of MVs, the dataset’s size, and the mechanism type of missingness. In addition, the results show that CBRSL can manipulate MVs generated from any missingness mechanism with a competitive accuracy against the compared methods.
    Type of Medium: Online Resource
    ISSN: 2079-9292
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2662127-7
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