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  • 2020-2024  (34)
  • 2000-2004  (8)
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  • 1
    Keywords: Forschungsbericht ; Ostsee ; Meeresboden ; Benthos ; Echolot ; Kartierung
    Type of Medium: Online Resource
    Pages: 1 Online-Ressource (60 Seiten, 4,77 MB) , Illustrationen, Diagramme
    Language: German
    Note: Förderkennzeichen BMBF 03F0768A-E , Verbundnummer 01176329 , Unterschiede zwischen dem gedruckten Dokument und der elektronischen Ressource können nicht ausgeschlossen werden
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  • 2
    Keywords: Hochschulschrift
    Type of Medium: Online Resource
    Pages: 1 Online-Ressource (71 Seiten = 13 MB) , Graphen
    Edition: Online-Ausgabe
    Language: English
    Note: Zusammenfassung in deutscher und englischer Sprache
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  • 3
    Keywords: Hochschulschrift
    Type of Medium: Online Resource
    Pages: 1 Online-Ressource (36 Seiten = 6 MB) , Graphen
    Edition: Online-Ausgabe
    Language: German
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Journal of applied phycology 12 (2000), S. 139-145 
    ISSN: 1573-5176
    Keywords: cellulose ; epiphytic bacteria ; Gracilaria conferta ; oligosaccharide ; peptide,plant-pathogen interactions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Certain forms of oligocellulose and certainbacterially excreted peptides were identified asendogenous and exogenous elicitors, respectively, ofa tip bleaching response in Gracilaria conferta(Schousboe ex Montagne) J. et G. Feldmann. Thehalf-maximal tip bleaching response was observed when31.1 μM cellobiose or 11.6 μM cellotetraosewere present in the growth medium. In contrast, noresponse was detected after exposure to glucose,cellotriose, cellopentaose or maltooligosaccharides.The response was thus strongly dependent on themolecular size of the oligocellulose and onlysaccharides that consisted of an even number ofglucose residues were elicitor-active. Three bacterialspecies that had earlier been identified as potentialinducers of the tip bleaching symptom excretedelicitor-active compounds into the growth medium.These compounds were protease-sensitive and thuspeptides or proteins. The tip bleaching-inducingcompound that was excreted by one Cytophaga-likeorganism was partially purified. It could be extractedfrom culture supernatants with chloroform and itsmolecular size was between 700 and 1500 Da,corresponding with a structure of 4–20 amino acids.Various endogenous and exogenous elicitors are thusrecognized by G. conferta and allow this alga torespond hypersensitively to the maceration of its cellwall skeleton or just to the presence of certainepiphytic organisms.
    Type of Medium: Electronic Resource
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  • 5
    Publication Date: 2024-04-23
    Description: The data were generated during an experiment simulating different frequencies of heatwaves (zero, one and three) in late spring/summer 2015. The experiment was carried out at the Kiel Outdoor Benthocosm (KOB) of GEOMAR Helmholtz Centre for Ocean Research Kiel, located at the Kiel Fjord. The biomass of filamentous algae was quantified from the most abundant genus occurring inside the tanks, i.e. Ceramium sp. The biomass of Zostera marina and Fucus vesiculosus was estimated from growth rates measurements carried out every 15 days. The biomass of all macrophytes was converted to carbon using specific carbon contents measured concomitantly with stable isotopes (https://doi.pangaea.de/10.1594/PANGAEA.966179). Respiration and primary production measurements were carried out on 09.08.2015 for filamentous algae, and on 12.08.2015 for Fucus vesiculosus. To execute these measurements, organisms were kept in gas-tight cylindrical chambers equipped with sensor spots for non-invasive oxygen measurements, which allowed continuous oxygen logging. Throughout the measurements, the chambers were kept inside the KOB tanks to maintain the temperature. The oxygen values were converted to carbon and normalized by the area of the tank (1.53 m2) per day. Note that the data of net primary production and respiration rates of the Z. marina were previously published (https://doi.org/10.1594/PANGAEA.904632). The carbon flux refers to the exports, i.e. biomass that was floating in the tanks, which was considered as carbon leaving (i.e. exported outside of) the system but still usable. The material to quantify the exports was collected every seven days, separated accounting for the contribution of each macrophyte group, dried at 80 °C until the biomass was constant and weighted. The dry weight was converted to carbon using the specific carbon contents measured concomitantly with stable isotopes (https://doi.pangaea.de/10.1594/PANGAEA.966179), and normalized by the area of the tank (1.53 m2) per day.
    Keywords: Amphipoda; Benthic biota; Biomass as carbon, export; Biomass as carbon, total per area; biomass estimation; carbon content; carbon fluxes; Experimental treatment; Fucus vesiculosus; Gastropoda; Isopoda; Kiel-Outdoor-Benthocosms; KOB; MESO; mesocosm experiment; Mesocosm experiment; net primary production; Net primary production of carbon; Respiration; Respiration rate, carbon; Stable carbon isotope (δ13C); stable nitrogen isotope (δ15N); Tank number; Taxa; Type of study; Zostera marina
    Type: Dataset
    Format: text/tab-separated-values, 264 data points
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  • 6
    Publication Date: 2020-06-11
    Description: As part of an ongoing research program aiming at monitoring molecular changes in the tissues and metabolite trafficking in the hydrosphere of algae subjected to chemical stresses, we are discussing the various analytical techniques that have been employed to characterize, and sometimes to quantity these metabolites. High-field multinuclear and solid-state nuclear magnetic resonance (NMR) spectroscopies are powerful tools for metabolite characterization from extracts and in vivo, but quantification and kinetic aspects show some limitations. Modern MS (mass spectrometry) is extremely useful for fingerprinting samples against databases and when dealing with very low concentrations of metabolites, the limitations being set by the type of chromatographic separation and mode of detection coupled with the mass spectrometer. Regarding chemical communication, optimization in terms of resolution and efficiency of hydrosphere chemical analysis can theoretically be achieved in a system which integrates (i) a multiparametric incubation chamber, (ii) a gasphase or a liquid-phase separation system and (iii) mass spectrometer(s) equipped with one or two detectors responding to the analytical and quantitative needs. This text reviews some of the techniques that have been employed in various types of plant metabolic studies, which may serve as a basis towards an integrative analytical strategy directly applicable to the metabolomics of selected marine macrophytes.
    Type: Article , PeerReviewed
    Format: text
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  • 7
    Publication Date: 2014-01-29
    Description: A review is presented of ongoing research on the oligosaccharide signals involved in cell-cell recognition in the Chondrus crispus-Acrochaete operculata host-pathogen association. In this pathosystem, the host gametophytes are resistant to the pathogen, whereas the sporophytic generation is susceptible to infection. The virulence of the green algal pathogen is mediated by the recognition of carrageenan oligosaccharides released from its red algal host: kappa-carrageenan oligosaccharides inhibit A. operculata virulence while lambda carrageenan oligosaccharides enhance its pathogenicity. It appears that the recognition of A. operculata by C. crispus also involves an oligosaccharidic signal. This signal is present in the non-virulent form of the pathogen whereas it is absent from the virulent form. Altogether this pathosystem offers a unique model to investigate the recognition of oligosaccharide signals in plant-pathogen interactions. The possible applications of this research to develop new strategies for disease control in maricultured algal crops are discussed.
    Type: Article , PeerReviewed
    Format: text
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  • 8
    Publication Date: 2020-07-20
    Description: Elicitation of Gracilaria conferta (Schousboe ex Montagne) J. ct G. Feldmann with oligoagars resulted in a defense response that was strong enough to kill epiphytic bacteria associated with the alga. Up to 60% of the resident bacterial flora of healthy plants was eliminated within 60 min after addition of neoagarohexaose to the algal medium. Single isolates of agar-degrading bacteria that had been isolated previously from healthy or decaying algal tissues proved to be more sensitive, Some of them were generally unable to survive on healthy G, conferta. Others survived on unelicited plants. Approximately 90% of these more resistant agar degraders were eliminated within 15 min after elicitation. The bacterial degradation of dead tissue of G, conferta resulted in a release of elicitors. The elicitors accumulated in the medium and reached high enough concentrations within 24 h to induce a hypersensitive response in healthy algae, The eliciting agent could be destroyed with beta -agarase and was thus probably oligoagar, Application of antibiotics prevented the accumulation of the elicitor, which indicated that bacteria were responsible for its release from the algal biomass, The hypersensitive response of G. conferta after contact with oligoagars is thus a true defense response, because it enables the plant to protect itself efficiently from enzymatic attacks on its cell wall.
    Type: Article , PeerReviewed
    Format: text
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  • 9
    Publication Date: 2014-01-29
    Description: The responses of Gracilaria lemaneiformis, an easily epiphytized host, and the relatively resistant G. cornea mutant, to the green alga Ulva lactuca were studied using biculture experiments with and without antibiotics. Both Gracilaria species grown with and without U. lactuca showed different levels of growth rate, release of hydrogen peroxide and of halogenated hydrocarbons. These quantitative differences led to a successful response against Ulva lactuca in the case of G. cornea mutant and to a failure in response in the case of G. lemaneiformis. The response of each Gracilaria species to U. lactuca was qualitatively similar to its response to bacteria. This suggests the involvement of oligosaccharide elicitors produced in the presence of epiphytes and bacteria. A clear Gracilaria inhibition was demonstrated with extracts of the culture medium. It appears that hydrogen peroxide, halogenated hydrocarbons and oligosaccharides may be components of the inhibitory activity of the extracts. The responses of Gracilaria species to the presence of U. lactuca suggest the characterization of a defence response.
    Type: Article , PeerReviewed
    Format: text
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  • 10
    Publication Date: 2020-07-27
    Description: A backfiring weapon: The release of H2O2 by an amino acid oxidase is the early defensive reaction of the red alga Chondrus crispus against the endophytic green algal pathogen Acrochaete operculata (see scheme). This reaction can be induced by L-asparagine, which is released by the attacker when it recognises host cell-wall κ-carrageenan oligosaccharides. The induced signal of the attacker thus serves directly as the substrate for the production of the defensive metabolite.
    Type: Article , PeerReviewed
    Format: text
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