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  • 1
    Publication Date: 2022-11-10
    Description: Dataset: Exudate Uptake Incubations - Microbial Abundances (Flow Cytometry)
    Description: Incubation experiments were conducted in St. John, US Virgin Islands to investigate the composition of exudates released from different species of benthic organisms, and the response of reef seawater microbial communities to mixed exudates released from different species and to specific metabolites. Exudates were collected from the stony coral Porites astreoides, and the octocoral Gorgonia ventalina after an 8 hour incubation. Reef seawater microbial communities were incubated separately in the presence of exudates from P. astreoides and G. ventalina for 48 hours and samples were collected to monitor changes in microbial abundance via flow cytometry and microbial community composition via 16S rRNA gene sequencing. Complementary Targeted and Untargeted metabolomic data from these incubation experiments is available on the MetaboLights database under accession number MTBLS2855. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/865739
    Description: NSF Division of Ocean Sciences (NSF OCE) OCE-1736288
    Keywords: coral reef ; Planktonic Microorganisms ; Prochlorococcus ; Synechococcus ; Cell Counts ; flow cytometry ; Microbial Communities ; 16S rRNA gene sequencing
    Repository Name: Woods Hole Open Access Server
    Type: Dataset
    Location Call Number Limitation Availability
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  • 2
    Publication Date: 2022-11-10
    Description: Dataset: Metabolite Uptake Incubations - Microbial Abundances (Flow Cytometry)
    Description: Pre-filtered reef seawater microbial communities collected from Lameshur Bay, U.S. Virgin Islands were incubated separately in the presence of the individual metabolites riboflavin, pantothenic acid, and caffeine for 24 hours and samples were collected to monitor changes in microbial community composition using 16S rRNA gene sequencing and microbial abundances using flow cytometry. Targeted metabolomic data from these incubations is available on the MetaboLights database under accession number MTBLS3286. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/865159
    Description: NSF Division of Ocean Sciences (NSF OCE) OCE-1736288
    Keywords: coral reef ; Planktonic Microorganisms ; Prochlorococcus ; Synechococcus ; Cell Counts ; flow cytometry ; Microbial Communities ; 16S rRNA gene sequencing
    Repository Name: Woods Hole Open Access Server
    Type: Dataset
    Location Call Number Limitation Availability
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  • 3
    Publication Date: 2022-10-20
    Description: © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Loranger, S., & Weber, T. C. . Shipboard acoustic observations of flow rate from a seafloor-sourced oil spill. Journal of Geophysical Research: Oceans, 125(10), (2020): e2020JC016274, https://doi.org/10.1029/2020JC016274.
    Description: In 2004 a debris flow generated by Hurricane Ivan toppled an oil production platform in Mississippi Canyon lease block 20 (MC20). Between 2004 and the installation of a containment system in 2019 MC20 became an in situ laboratory for a wide range of hydrocarbon in the sea‐related research, including different methods of assessing the volumetric flow rate of hydrocarbons spanning different temporal scales. In 2017 a shipboard acoustic Doppler current profiler (ADCP) and high‐frequency (90 to 154 kHz) broadband echosounder were deployed to assess the flow rate of liquid and gas phase hydrocarbons. Measurements of horizontal currents were combined with acoustic mapping to determine the rise velocity of the seep as it moved downstream. Models of the rise velocity for fluid particles were used to predict the size of oil droplets and gas bubbles in the seep. The amplitude and shape of the broadband acoustic backscatter were then used to differentiate between, and determine the flow rate of, hydrocarbons. Oil flow rate in the seep was estimated to be 56 to 86 barrels/day (mean urn:x-wiley:jgrc:media:jgrc24228:jgrc24228-math-0001 barrels/day) while the flow rate of gaseous hydrocarbons was estimated to be 98 to 359 m3/day (mean urn:x-wiley:jgrc:media:jgrc24228:jgrc24228-math-0002 m3/day).
    Description: The work was supported by the National Oceanic and Atmospheric Administration (Grant NA15NOS4000200).
    Keywords: Oil spill ; Acoustics ; Flow rate ; MC20
    Repository Name: Woods Hole Open Access Server
    Type: Article
    Location Call Number Limitation Availability
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