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  • 2020-2023  (3)
  • 2010-2014  (7)
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  • 1
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    Potentials for offset approaches in selected sectors post 2020 (2022)
    Dessau-Roßlau : Umweltbundesamt
    Details
    Publication Date: 2022-08-15
    Description: This report develops an evaluation framework that policymakers can use to identify whether offsets can add value and uphold environmental integrity of a compliance scheme. It uses a scoring framework on factors to: (1) identify which sectors have hard-to-abate emissions that can justify demanding offsets as cost-containment measures for ambitious climate policies; and (2) identify mitigation activities that are otherwise inaccessible, fosters sustainable development, and the extent to which it enables transformative sectoral action to be eligible to supply offsets. This evaluation framework identifies the optimal conditions that make factors successful in either having sectors demand offsets, or specific mitigation activities supply offsets. Sectoral emissions that are hard-to-abate are those that are technically unavoidable due to a lack and maturity of technologies, and therefore should be allowed to have cost-containment measures - such as offsets - to avoid adverse economic ramifications such as carbon leakage. Mitigation activities that can supply offsets are those that are currently inaccessible to local actor’s due to lack of access to technology, finance or capabilities. Allowing these mitigation activities to be eligible to supply offsets allows to pilot such activities and realize mitigation outcomes outside the original scope of the compliance scheme. This report has chosen selected sectors and mitigation activities to illustrate how this framework can be applied at the global level. It recognizes that country-specific factors can change the assessment of whether the offset approach will add value and uphold environmental integrity to proposed compliance schemes of a country. The report further proposes practical steps policymakers can do to undertake an evaluation at the national level.
    Keywords: ddc:320
    Repository Name: Wuppertal Institut für Klima, Umwelt, Energie
    Language: English
    Type: report , doc-type:report
    Format: application/pdf
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  • 2
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    Suitability and success factors of offsets post-2020 (2022)
    Dessau-Roßlau : Umweltbundesamt
    Details
    Publication Date: 2022-08-15
    Description: Offsetting enables countries and companies to meet part of their climate change mitigation obligations by using mitigation outcomes generated elsewhere - in lieu of own emission reductions. This report explores the future role of offset approaches and how they could be successfully integrated into a post-2020 climate regime by focusing both the supply and demand side. For this purpose, the report develops a conceptual approach that derives a normative vision of what should be considered a successful offset use in a top-down manner to then link this vision to specific factors on the ground in sectors and jurisdictions where offsets will be generated and used. It explores how these factors influence the successful operationalisation of the offset approach and how they can inform its design. In addition, the report also explores six conceptual design aspects to providing recommendations on how to take these factors into account during the design of the offset approach. Based on these findings, the authors derive overarching policy recommendations on the integration of offsets into carbon pricing schemes.
    Keywords: ddc:320
    Repository Name: Wuppertal Institut für Klima, Umwelt, Energie
    Language: English
    Type: report , doc-type:report
    Format: application/pdf
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  • 3
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    Offset approaches in existing compliance mechanisms : adding value and upholding environmental integrity? (2022)
    Dessau-Roßlau : Umweltbundesamt
    Details
    Publication Date: 2022-08-15
    Description: The objective of this report is to use historical analysis to identify conditions that determine when offsets add value to compliance schemes while upholding environmental integrity. The indicators of success include: increased acceptance of introducing compliance schemes; raising ambition in subsequent compliance periods; the possibility to drive emission reductions outside the compliance sectors; promoting investments in sustainable development; and avoiding perverse incentives that undermine the stringency of the compliance scheme or compliance actors’ efforts in reducing their own emissions. Through undertaking in-depth case study analyzes on the effects of offsets in the European Union, Alberta, Australia, Colombia and Japan, the report identifies common conditions that explain why offsets were successful (or not) in achieving individual indicators. The report further identifies two common conditions that can help explain when offsets achieve all five indicators of success. The first is that policymakers need to be willing to design the compliance scheme to set and maintain a strong compliance price signal that justifies the need for incorporating cost containment measures, such as offsets, to avert negative political and economic ramifications. Relatedly, the second condition requires institutions, processes and infrastructure that govern both the compliance scheme and offsets to be well developed so that they can ensure offsets uphold the principles of environmental integrity, achieve sustainable development benefits, and act as a reliable cost containment measure to high compliance prices. The findings also highlight how difficult it is to achieve both conditions, as both domestic and international political economy factors determine whether policymakers and voters are willing to introduce and maintain compliance schemes that deliver effective action on climate.
    Keywords: ddc:320
    Repository Name: Wuppertal Institut für Klima, Umwelt, Energie
    Language: English
    Type: report , doc-type:report
    Format: application/pdf
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  • 4
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    Evaluation of six different DNA extraction methods for detection of Mycobacterium tuberculosis by means of PCR-IS6110: preliminary study (2013)
    BioMed Central
    Details
    Publication Date: 2013-12-29
    Description: Background: Developments in molecular detection and strain differentiation of members of Mycobacterium tuberculosis complex have proved to be useful. The DNA extraction method influences the amplification efficiency, causing interference on the sensitivity and respective inhibitors. The aim of this study was to standardize a simple and fast DNA extraction method, providing DNA amplification by IS6110-PCR effectively free from undue interferences.FindingsThe efficiency of the six different protocols tested in M. tuberculosis cultures has varied from 75 % to 92.5 %. This preliminary study evaluating the IS6110 PCR sensitivity and specificity was developed in DNA extracted from microscope slides, and achieved 100 % of efficiency. Conclusions: DNA extraction by Chelex + NP-40 method from both, cultures of M. tuberculosis and smear slides, resulted in good quantity of interference free DNA, especially in samples with low concentrations of genetic material; therefore, such technique may be used for the molecular diagnosis of tuberculosis.
    Electronic ISSN: 1756-0500
    Topics: Biology , Medicine
    Published by BioMed Central
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  • 5
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    Cell therapy for fibrotic interstitial pulmonary disease: Experimental study (2011)
    Wiley-Blackwell
    Details
    Publication Date: 2011-01-05
    Description: Parte superior do formulário Digite um texto ou endereço de um site ou traduza um documento. The aim of this study is to evaluate the histological changes in lung parenchyma of pigs affected by interstitial lung disease induced after the infusion of bone marrow mononuclear cells (BMMCs). Ten female swines were submitted to pulmonary fibrosis induced by a single dose of intratracheal bleomicine sulfate. Animals were arranged into two groups: Group 1: induced-disease control and Group 2: cell therapy using BMMCs. Both groups were clinically evaluated for 180 days. High-resolution computed tomography (HRCT) was performed at 90 and 180 days. BMMC sampling was performed in cell therapy group at 90 days. Euthanasia was performed, and samples were collected for histology and immunohistochemistry. The 90-days HRCT demonstrated typical interstitial lesions in pulmonary parenchyma similarly to human disease. The 180-days HRCT in Group 1 demonstrated advanced stages of the disease when compared with Group 2. Immunohistochemistry analysis suggests the presence of pre-existent vessels and neoformed vessels as well as predominant young cells in the injured parenchyma of Group 2. Immunohistochemistry analysis suggests that cell therapy would promote a reconstructive response. Histology and HRCT analysis suggest a positive application of swine as a model for a bleomicine inducing of fibrotic interstitial pulmonary disease. Microsc. Res. Tech., 2011. © 2011 Wiley-Liss, Inc.
    Print ISSN: 1059-910X
    Electronic ISSN: 1097-0029
    Topics: Natural Sciences in General
    Published by Wiley-Blackwell
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  • 6
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    Macroscopic and microscopic analysis of the tongue of the common opossum (Didelphis marsupialis) (2012)
    Wiley-Blackwell
    Details
    Publication Date: 2012-05-13
    Description: We performed a macroscopic and microscopic study of the tongues of common opossums, Didelphis marsupialis, from South America. We studied two males and two females. We collected morphometric data on the tongue with precision calipers. For the light microscopy and scanning electron microscopy analyses, we fixed tissue fragments in 10% formaldehyde and 2.5% glutaraldehyde, respectively. The opossum tongues averaged 5.87 ± 0.20 cm in length, 3.27 ± 0.15 cm in width at the lingual body, and 3.82 ± 0.15 cm in width at the root. The mean thickness of the lingual body was 1.8 ± 0.1 cm, and the thickness of the root was 3.82 ± 0.15 cm. Sharp filiform papillae were scattered across the entire tongue; conical filiform papillae occurred on the lingual body and tongue tip; fungiform papillae were scattered among the filiform papillae on the lingual body and tongue tip; and there were three vallate papillae at the root of the tongue. We found two strands of papillary projections in the tongue root. Despite the low variability observed in the lingual papillae, the morphological data obtained in this study may be related to the opossum's diverse food habits and the extensive geographic distribution of the species throughout America. Microsc. Res. Tech. 2012. © 2012 Wiley Periodicals, Inc.
    Print ISSN: 1059-910X
    Electronic ISSN: 1097-0029
    Topics: Natural Sciences in General
    Published by Wiley-Blackwell
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  • 7
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    Ordering of mutations in preinvasive disease stages of esophageal carcinogenesis (2014)
    Nature Publishing Group (NPG)
    Details
    Publication Date: 2014-07-30
    Description: Nature Genetics 46, 837 (2014). doi:10.1038/ng.3013 Authors: Jamie M J Weaver, Caryn S Ross-Innes, Nicholas Shannon, Andy G Lynch, Tim Forshew, Mariagnese Barbera, Muhammed Murtaza, Chin-Ann J Ong, Pierre Lao-Sirieix, Mark J Dunning, Laura Smith, Mike L Smith, Charlotte L Anderson, Benilton Carvalho, Maria O'Donovan, Timothy J Underwood, Andrew P May, Nicola Grehan, Richard Hardwick, Jim Davies, Arusha Oloumi, Sam Aparicio, Carlos Caldas, Matthew D Eldridge, Paul A W Edwards, Nitzan Rosenfeld, Simon Tavaré & Rebecca C Fitzgerald
    Print ISSN: 1061-4036
    Electronic ISSN: 1546-1718
    Topics: Biology , Medicine
    Published by Nature Publishing Group (NPG)
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  • 8
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    Undergraduate research in medical education: a descriptive study of students' views (2014)
    BioMed Central
    Details
    Publication Date: 2014-03-18
    Description: Background: Medical students engage in curricular and extracurricular activities, including undergraduate research (UR). The advantages, difficulties and motivations for medical students pursuing research activities during their studies have rarely been addressed. In Brazil, some medical schools have included undergraduate research into their curriculum. The present study aimed to understand the reality of scientific practice among medical students at a well-established Brazilian medical school, analyzing this context from the students' viewpoint. Methods: A cross-sectional survey based on a questionnaire applied to students from years one to six enrolled in an established Brazilian medical school that currently has no curricular UR program. Results: The questionnaire was answered by 415 students, 47.2% of whom were involved in research activities, with greater participation in UR in the second half of the course. Independent of student involvement in research activities, time constraints were cited as the main obstacle to participation. Among students not involved in UR, 91.1% said they favored its inclusion in the curriculum, since this would facilitate the development of such activity. This approach could signify an approximation between the axes of teaching and research. Among students who had completed at least one UR project, 87.7% said they would recommend the activity to students entering the course. Conclusion: Even without an undergraduate research program, students of this medical school report strong involvement in research activities, but discussion of the difficulties inherent in its practice is important to future developments.
    Electronic ISSN: 1472-6920
    Topics: Medicine , Education
    Published by BioMed Central
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  • 9
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    Type IV Secretion System Is Not Involved in Infection Process in Citrus (2014)
    Hindawi
    Details
    Publication Date: 2014-02-24
    Description: The type IV secretion system (T4SS) is used by Gram-negative bacteria to translocate protein and DNA substrates across the cell envelope and into target cells. Xanthomonas citri subsp. citri contains two copies of the T4SS, one in the chromosome and the other is plasmid-encoded. To understand the conditions that induce expression of the T4SS in Xcc, we analyzed, in vitro and in planta, the expression of 18 ORFs from the T4SS and 7 hypothetical flanking genes by RT-qPCR. As a positive control, we also evaluated the expression of 29 ORFs from the type III secretion system (T3SS), since these genes are known to be expressed during plant infection condition, but not necessarily in standard culture medium. From the 29 T3SS genes analyzed by qPCR, only hrpA was downregulated at 72 h after inoculation. All genes associated with the T4SS were downregulated on Citrus leaves 72 h after inoculation. Our results showed that unlike the T3SS, the T4SS is not induced during the infection process.
    Print ISSN: 1687-918X
    Electronic ISSN: 1687-9198
    Topics: Biology , Medicine
    Published by Hindawi
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  • 10
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    Evaluation of single nucleotide polymorphisms in microRNAs (hsa-miR-196a2 rs11614913 C/T) from Brazilian women with breast cancer (2012)
    BioMed Central
    Details
    Publication Date: 2012-12-11
    Description: Background: Emerging evidence has shown that miRNAs are involved in human carcinogenesis as tumor suppressors or oncogenes. Single nucleotide polymorphisms (SNPs) located in pre-miRNAs may affect the processing and therefore, influence the expression of mature miRNAs. Previous studies generated conflicting results when reporting association between the hsa-miR-196a2 rs11614913 common polymorphism and breast cancer. Methods: This study evaluated the hsa-miR-196a2 rs11614913 SNP in 388 breast cancer cases and 388 controls in Brazilian women. Polymorphism was determined by real-time PCR; control and experimental groups were compared through statistical analysis using the X2 or Fisher's exact tests. Results: The analysis of the SNPs frequencies showed a significant difference between the groups (BC and CT) in regards to genotype distribution (chi2: p = 0.024); the homozygous variant (TC) was more frequent in the CT than in the BC group (p = 0.009). The presence of the hsa-miR-196a2 rs11614913 C/T polymorphism was not associated with histological grades (p = 0.522), axillary lymph node positive status (p = 0.805), or clinical stage (p = 0.670) among the breast cancer patients. Conclusions: The results of this study indicated that the CC polymorphic genotype is associated with a decreased risk of BC and the presence of the T allele was significantly associated with an increased risk of BC.
    Electronic ISSN: 1471-2350
    Topics: Biology , Medicine
    Published by BioMed Central
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