In:
PLOS ONE, Public Library of Science (PLoS), Vol. 17, No. 6 ( 2022-6-16), p. e0269984-
Abstract:
Previous studies in the liverwort Marchantia polymorpha have shown that the putative evening complex (EC) genes LUX ARRHYTHMO ( LUX ) and ELF4-LIKE ( EFL ) have a function in the liverwort circadian clock. Here, we studied the growth phenotypes of Mp LUX and Mp EFL loss-of-function mutants, to establish if PHYTOCHROME-INTERACTING FACTOR ( PIF ) and auxin act downstream of the M . polymorpha EC in a growth-related pathway similar to the one described for the flowering plant Arabidopsis. We examined growth rates and cell properties of loss-of-function mutants, analyzed protein-protein interactions and performed gene expression studies using reporter genes. Obtained data indicate that an EC can form in M . polymorpha and that this EC regulates growth of the thallus. Altered auxin levels in Mp lux mutants could explain some of the phenotypes related to an increased thallus surface area. However, because Mp PIF is not regulated by the EC, and because Mp pif mutants do not show reduced growth, the growth phenotype of EC-mutants is likely not mediated via Mp PIF . In Arabidopsis, the circadian clock regulates elongation growth via PIF and auxin, but this is likely not an evolutionarily conserved growth mechanism in land plants. Previous inventories of orthologs to Arabidopsis clock genes in various plant lineages showed that there is high levels of structural differences between clocks of different plant lineages. Here, we conclude that there is also variation in the output pathways used by the different plant clocks to control growth and development.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0269984
DOI:
10.1371/journal.pone.0269984.g001
DOI:
10.1371/journal.pone.0269984.g002
DOI:
10.1371/journal.pone.0269984.g003
DOI:
10.1371/journal.pone.0269984.g004
DOI:
10.1371/journal.pone.0269984.g005
DOI:
10.1371/journal.pone.0269984.g006
DOI:
10.1371/journal.pone.0269984.g007
DOI:
10.1371/journal.pone.0269984.s001
DOI:
10.1371/journal.pone.0269984.s002
DOI:
10.1371/journal.pone.0269984.s003
DOI:
10.1371/journal.pone.0269984.s004
DOI:
10.1371/journal.pone.0269984.s005
DOI:
10.1371/journal.pone.0269984.s006
DOI:
10.1371/journal.pone.0269984.s007
DOI:
10.1371/journal.pone.0269984.s008
DOI:
10.1371/journal.pone.0269984.s009
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2267670-3
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