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  • 2015-2019  (17)
  • 2016  (17)
  • 1
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    Observation of “wired” cell communication over 10-μm and 20-μm poly(dimethylsiloxane) barriers in tetracycline inducible expression systems (2016)
    American Institute of Physics (AIP)
    Details
    Publication Date: 2016-01-15
    Description: Communication between cells and extracellular environments is of interest because of its critical roles in cell development and differentiation. Particularly, this signal transduction is commonly believed to rely on the contact and binding of the participating molecules/proteins, suggesting that the binding distance needed is less than a few nanometers. However, it is difficult to precisely match the rapidly binding interaction which depends on the probability of molecular collision in living systems, raising a hypothesis that another mechanism exists, could promote this signal communication, and remains unknown. Here we report that a long-range signal delivery over 10- μ m and 20- μ m polydimethylsiloxane (PDMS) barriers can be observed in microfluidically tetracycline (Tet) inducible expression systems. Results show that a significant increment of the long-range induced green fluorescent protein in human embryonic kidney 293T (HEK 293T) cells by the stimulation of Tet is demonstrated, and that such a signal induction is not dominated by Tet diffusion and displays a specific bindingless property. In addition, our experimental results, combined with theoretical modeling, suggest that this communication exhibits a bump-shaped characteristic depending on barrier thickness, materially structural property, surface roughness, and agonist concentration. It strongly relies on the PDMS barrier to delivery signal; therefore, we call such a mechanism as “wired” cell communication instead of wireless. These results could ignite interests in the novel and “wired” cell communication, which we call it X-signal , and in the use of such systems for the study of cellular biology and development of new drug.
    Print ISSN: 0021-8979
    Electronic ISSN: 1089-7550
    Topics: Physics
    Published by American Institute of Physics (AIP)
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  • 2
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    Tracing haematopoietic stem cell formation at single-cell resolution (2016)
    Nature Publishing Group (NPG)
    In: Nature
    Details
    Publication Date: 2016-05-26
    Description: Tracing haematopoietic stem cell formation at single-cell resolution Nature 533, 7604 (2016). doi:10.1038/nature17997 Authors: Fan Zhou, Xianlong Li, Weili Wang, Ping Zhu, Jie Zhou, Wenyan He, Meng Ding, Fuyin Xiong, Xiaona Zheng, Zhuan Li, Yanli Ni, Xiaohuan Mu, Lu Wen, Tao Cheng, Yu Lan, Weiping Yuan, Fuchou Tang & Bing Liu Haematopoietic stem cells (HSCs) are derived early from embryonic precursors, such as haemogenic endothelial cells and pre-haematopoietic stem cells (pre-HSCs), the molecular identity of which still remains elusive. Here we use potent surface markers to capture the nascent pre-HSCs at high purity, as rigorously validated
    Print ISSN: 0028-0836
    Electronic ISSN: 1476-4687
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Published by Nature Publishing Group (NPG)
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  • 3
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    Protective effect of wogonin on endotoxin-induced acute lung injury via reduction of p38 MAPK and JNK phosphorylation (2016)
    Wiley-Blackwell
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    Publication Date: 2016-02-20
    Description: ABSTRACT Acute lung injury (ALI) is a serious inflammatory disorder which remains the primary cause of incidence and mortality in patients with acute pulmonary inflammation. However, there is still no effective medical strategy available clinically for the improvement of ALI. Wogonin, isolated from roots of Scutellaria baicalensis Georgi, is a common medicinal herb which presents biological and pharmacological effects, including antioxidation, anti-inflammation, and anticancer. Preadministration of wogonin inhibited not only lung edema but also protein leakage into the alveolar space in murine model of lipopolysaccharide (LPS)-induced ALI. Moreover, wogonin not only reduced the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)−2 but also inhibited the phosphorylation of mitogen-activated protein kinase (MAPK) induced by LPS. We further found wogonin inhibited the phosphorylation of p38 MAPK and JNK at a concentration lower than ERK. In addition, inhibition of lung edema, protein leakage, expression of iNOS and COX-2, and phosphorylation of p38 MAPK and JNK were all observed in a parallel concentration-dependent manner. These results suggest that wogonin possesses potential protective effect against LPS-induced ALI via downregulation of iNOS and COX-2 expression by blocking phosphorylation of p38 MAPK and JNK. © 2016 Wiley Periodicals, Inc. Environ Toxicol, 2016.
    Print ISSN: 1520-4081
    Electronic ISSN: 1522-7278
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Published by Wiley-Blackwell
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  • 4
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    Cultural perspectives of older nursing home residents regarding signing their own DNR directives in Eastern Taiwan: a qualitative pilot study (2016)
    BioMed Central
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    Publication Date: 2016-05-08
    Description: Chinese tradition and culture developed from Taoism, Confucianism, and Buddhism and have influenced ethnic Chinese for thousands of years, particularly thoughts on death. Many ethnic Chinese, particularly olde...
    Electronic ISSN: 1472-684X
    Topics: Medicine
    Published by BioMed Central
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  • 5
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    Energies, Vol. 9, Pages 625: Modeling of Load Bearing Characteristics of Jacket Foundation Piles for Offshore Wind Turbines in Taiwan (2016)
    MDPI Publishing
    In: Energies
    Details
    Publication Date: 2016-08-10
    Description: This paper presents a pioneering study on numerical modeling of load bearing characteristics of the jacket foundation pile for offshore wind turbines on the west coast of Taiwan. Because Taiwan is located in an earthquake prone area, there is significant interest in improving the prediction of the behavior of wind turbine jacket foundations subjected to seismic loading. Investigation of the bearing capacity of the jacket foundation pile for the offshore wind farm using effective stress analysis, with consideration of pore pressure generation and soil/liquid coupled analysis, was conducted. A new procedure to evaluate the design of offshore wind turbine foundation piles in the sand and clay inter-layered soil was also proposed. Static and dynamic analyses of bearing capacity of the jacket foundation pile were conducted. Results obtained demonstrate that the design process for the jacket foundation pile proposed in this study can properly reflect the interaction behavior of the foundation and the soil. In addition, the pore pressure generation model can be used to simulate soil liquefaction. The proposed method is also very useful in the evaluation of the design capabilities of offshore wind turbine jacket foundations.
    Electronic ISSN: 1996-1073
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Published by MDPI Publishing
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  • 6
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    Symmetry, Vol. 8, Pages 104: A Search Complexity Improvement of Vector Quantization to Immittance Spectral Frequency Coefficients in AMR-WB Speech Codec (2016)
    MDPI Publishing
    In: Symmetry
    Details
    Publication Date: 2016-10-01
    Description: An adaptive multi-rate wideband (AMR-WB) code is a speech codec developed on the basis of an algebraic code-excited linear-prediction (ACELP) coding technique, and has a double advantage of low bit rates and high speech quality. This coding technique is widely used in modern mobile communication systems for a high speech quality in handheld devices. However, a major disadvantage is that a vector quantization (VQ) of immittance spectral frequency (ISF) coefficients occupies a significant computational load in the AMR-WB encoder. Hence, this paper presents a triangular inequality elimination (TIE) algorithm combined with a dynamic mechanism and an intersection mechanism, abbreviated as the DI-TIE algorithm, to remarkably improve the complexity of ISF coefficient quantization in the AMR-WB speech codec. Both mechanisms are designed in a way that recursively enhances the performance of the TIE algorithm. At the end of this work, this proposal is experimentally validated as a superior search algorithm relative to a conventional TIE, a multiple TIE (MTIE), and an equal-average equal-variance equal-norm nearest neighbor search (EEENNS) approach. With a full search algorithm as a benchmark for search load comparison, this work provides a search load reduction above 77%, a figure far beyond 36% in the TIE, 49% in the MTIE, and 68% in the EEENNS approach.
    Electronic ISSN: 2073-8994
    Topics: Mathematics , Physics
    Published by MDPI Publishing
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  • 7
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    IJERPH, Vol. 13, Pages 553: Empirical Model for Evaluating PM10 Concentration Caused by River Dust Episodes (2016)
    MDPI Publishing
    Details
    Publication Date: 2016-06-03
    Description: Around the estuary of the Zhuo-Shui River in Taiwan, the waters recede during the winter, causing an increase in bare land area and exposing a large amount of fine earth and sand particles that were deposited on the riverbed. Observations at the site revealed that when northeastern monsoons blow over bare land without vegetation or water cover, the fine particles are readily lifted by the wind, forming river dust, which greatly endangers the health of nearby residents. Therefore, determining which factors affect river dust and constructing a model to predict river dust concentration are extremely important in the research and development of a prototype warning system for areas at risk of river dust emissions. In this study, the region around the estuary of the Zhuo-Shui River (from the Zi-Qiang Bridge to the Xi-Bin Bridge) was selected as the research area. Data from a nearby air quality monitoring station were used to screen for days with river dust episodes. The relationships between PM10 concentration and meteorological factors or bare land area were analyzed at different temporal scales to explore the factors that affect river dust emissions. Study results showed that no single factor alone had adequate power to explain daily average or daily maximum PM10 concentration. Stepwise regression analysis of multiple factors showed that the model could not effectively predict daily average PM10 concentration, but daily maximum PM10 concentration could be predicted by a combination of wind velocity, temperature, and bare land area; the coefficient of determination for this model was 0.67. It was inferred that river dust episodes are caused by the combined effect of multiple factors. In addition, research data also showed a time lag effect between meteorological factors and hourly PM10 concentration. This characteristic was applied to the construction of a prediction model, and can be used in an early warning system for local residents.
    Print ISSN: 1661-7827
    Electronic ISSN: 1660-4601
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Published by MDPI Publishing
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  • 8
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    Fluorescence Detection of H5N1 Virus Gene Sequences Based on Optical Tweezers with Two-Photon Excitation Using a Single Near Infrared Nanosecond Pulse Laser (2016)
    American Chemical Society (ACS)
    Details
    Publication Date: 2016-04-07
    Description: Analytical Chemistry DOI: 10.1021/acs.analchem.6b00065
    Print ISSN: 0003-2700
    Electronic ISSN: 1520-6882
    Topics: Chemistry and Pharmacology
    Published by American Chemical Society (ACS)
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  • 9
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    KRAS mutation coupled with p53 loss is sufficient to induce ovarian carcinosarcomas in mice (2016)
    Wiley-Blackwell
    Details
    Publication Date: 2016-12-30
    Description: Ovarian carcinosarcoma cancer is the most lethal form of gynecological malignancy, but the pathogenesis and biological function for this ovarian cancer remain unknown. We establishment the transgenic mouse model of K-ras G12D p53 loxP/loxP and found that K-ras mutation and p53 deletion within the ovarian surface epithelium gave rise to ovarian lesions with a hyperproliferation and endometrioid glandular morphology. Furthermore, double mutant ovaries formed ovarian carcinosarcomas that were high grade and poorly differentiated. Induction was widely metastatic and spread to abdominal organs including liver, spleen, and kidney at 4 wk. We also confirmed the role of K-ras G12D in ovarian cancer cell lines MCAS and PA-1 and showed that K-ras G12D overexpression strongly induced cell proliferation, migration, and invasion. The ovarian cancer model we developed recapitulates the specific tumor histomorphology and the probable mechanism of malignant transformation in endometriosis. This article is protected by copyright. All rights reserved.
    Print ISSN: 0020-7136
    Electronic ISSN: 1097-0215
    Topics: Biology , Medicine
    Published by Wiley-Blackwell
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  • 10
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    Sustainability, Vol. 9, Pages 45: China’s New Urban Space Regulation Policies: A Study of Urban Development Boundary Delineations (2016)
    MDPI Publishing
    Details
    Publication Date: 2016-12-30
    Description: China’s rapid urbanisation has led to ecological deterioration and reduced the land available for agricultural production. The purpose of this study is to develop an urban development boundary delineation (UDBD) model using the high-tech manufacturing area of Xinbei in the District of Changzhou as a case study, and by applying remote sensing, GIS, and other technologies. China’s UDBD policies are reviewed, spatiotemporal changes since 1985 are documented, and future expansion is modelled to 2020. The simulated urban-growth patterns are analysed in relation to China’s policies for farmland preservation, ecological redlines protection areas, and housing developments. The UDBD model developed in this study satisfies regional farmland and ecological space protection constraints, while being consistent with urban development strategies. This study provides theoretical references and technological support for the implementation of land management policies that will optimize land allocations for urban growth, agriculture, and ecological protection.
    Electronic ISSN: 2071-1050
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Published by MDPI Publishing
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