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  • 2010-2014  (16)
  • 2005-2009
  • 2014  (16)
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  • 2010-2014  (16)
  • 2005-2009
Year
  • 1
    Publication Date: 2014-07-21
    Description: Highlights • First long-term experiments on effects of high pCO2 and temperature on Calanus spp. • CO2 concentration of 3000 μatm had no effect on the copepods performance. • Temperature of 10 °C induced sublethal stress in diapausing C. hyperboreus females. • Synergistic effects of temperature and CO2 on body carbon were found at 5 °C. Abstract The sensitivity of copepods to ocean acidification (OA) and warming may increase with time, however, studies 〉10 days and on synergistic effects are rare. We therefore incubated late copepodites and females of two dominant Arctic species, Calanus glacialis and Calanushyperboreus, at 0 °C at 390 and 3000 μatm pCO2 for several months in fall/winter 2010. Respiration rates, body mass and mortality in both species and life stages did not change with pCO2. To detect synergistic effects, in 2011 C. hyperboreus females were kept at different pCO2 and temperatures (0, 5, 10 °C). Incubation at 10 °C induced sublethal stress, which might have overruled effects of pCO2. At 5 °C and 3000 μatm, body carbon was significantly lowest indicating a synergistic effect. The copepods, thus, can tolerate pCO2 predicted for a future ocean, but in combination with increasing temperatures they could be sensitive to OA.
    Type: Article , PeerReviewed
    Format: text
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  • 2
    Publication Date: 2016-05-02
    Type: Conference or Workshop Item , NonPeerReviewed
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  • 3
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    In:  [Talk] In: APECS Netherlands Symposium, 04.11.2014, Groningen, The Netherlands .
    Publication Date: 2016-05-02
    Type: Conference or Workshop Item , NonPeerReviewed
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  • 4
    Publication Date: 2016-05-02
    Type: Conference or Workshop Item , NonPeerReviewed
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  • 5
    Publication Date: 2023-03-07
    Description: The present study aimed to contribute to the knowledge on the intraspecific variations of enzyme activities in populations of Calanus finmarchicus from different longitudes across the North Atlantic Ocean and their relation to changing environmental conditions. C. finmarchicus was sampled across the North Atlantic in basins with decreasing temperature regimes from east to west (Iceland Basin, Irminger Basin and Labrador Basin) in late March/early April 2013. Potential maximum enzyme activities of digestive (proteinases and lipases/esterases) and metabolic (citrate synthase) enzymes of copepods from all sampling stations were analysed and thermal profiles (5-50°C) of enzyme activities were determined. In order to investigate its acclimation potential, C. finmarchicus were acclimated to 4°C and 15°C for two weeks and thermal profiles of enzyme activities were compared afterwards.
    Keywords: Basin Scale Analysis, Synthesis and Integration; EURO-BASIN
    Type: Dataset
    Format: application/zip, 3 datasets
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  • 6
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    PANGAEA
    In:  Supplement to: Hildebrandt, Nicole; Niehoff, Barbara; Sartoris, Franz-Josef (2014): Long-term effects of elevated CO2 and temperature on the Arctic calanoid copepods Calanus glacialis and C. hyperboreus. Marine Pollution Bulletin, 80(1-2), 59-70, https://doi.org/10.1016/j.marpolbul.2014.01.050
    Publication Date: 2024-03-15
    Description: The sensitivity of copepods to ocean acidification (OA) and warming may increase with time, however, studies 〉10 days and on synergistic effects are rare. We therefore incubated late copepodites and females of two dominant Arctic species, Calanus glacialis and Calanus hyperboreus, at 0 °C at 390 and 3000 µatm pCO2 for several months in fall/winter 2010. Respiration rates, body mass and mortality in both species and life stages did not change with pCO2. To detect synergistic effects, in 2011 C. hyperboreus females were kept at different pCO2 and temperatures (0, 5, 10 °C). Incubation at 10 °C induced sublethal stress, which might have overruled effects of pCO2. At 5 °C and 3000 µatm, body carbon was significantly lowest indicating a synergistic effect. The copepods, thus, can tolerate pCO2 predicted for a future ocean, but in combination with increasing temperatures they could be sensitive to OA.
    Keywords: Alkalinity, total; Animalia; Aragonite saturation state; Arctic; Arthropoda; Bicarbonate ion; BIOACID; Biological Impacts of Ocean Acidification; Bottles or small containers/Aquaria (〈20 L); Calanus glacialis; Calanus hyperboreus; Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbon/Nitrogen ratio; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Carbon mass; Coulometric titration; Dry mass; Experiment day; Figure; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gonadal stage; Growth/Morphology; Laboratory experiment; Length; Life stage; Mortality; Mortality/Survival; Nitrogen mass; OA-ICC; Ocean Acidification International Coordination Centre; Open ocean; Oxygen consumption; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH; pH, standard deviation; Polar; Potentiometric; Replicates; Reproduction; Respiration; Salinity; Salinity, standard deviation; Single species; Species; Temperature; Temperature, water; Temperature, water, standard deviation; Treatment; Treatment: temperature; Zooplankton
    Type: Dataset
    Format: text/tab-separated-values, 51067 data points
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  • 7
    Publication Date: 2024-02-02
    Description: The prosome length of copepods from each station was measured on board with a dissecting microscope equipped with an ocular micrometer. Individuals were placed in pre-weighed tin caps and dried for 48 h at 60°C on board. Dry samples were transferred to the AWI and weighed again. Copepod dry mass was then calculated as the difference between the empty weight and the weight of the tin cap containing one individual. The content of carbon (C) and nitrogen (N) then was analysed with a CN-analyser (EuroEA Element Analyser, Hekatech) with acetanilide as standard.
    Keywords: Basin Scale Analysis, Synthesis and Integration; Carbon content per individual; Date/Time of event; D-MOC; Double opening/closing plankton net; Element analyser CNS, EURO EA; EURO-BASIN; Event label; Individual dry mass; Latitude of event; Life stage; Longitude of event; Maria S. Merian; Measured; MSM26; MSM26_126-9; MSM26_127-17; MSM26_131-17; MSM26_134-19; MSM26_135-16; MSM26_136-8; Nitrogen content per individual; North Atlantic; Prosome, length; Sample ID; Taxon/taxa; Uniform resource locator/link to reference; Weighted
    Type: Dataset
    Format: text/tab-separated-values, 1152 data points
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  • 8
    Publication Date: 2024-02-02
    Description: For the determination of water-soluble protein content of C. finmarchicus of the different stations the Qubit® Protein Assay Kit (Invitrogen) was used. Analysis was performed with extracts of 10 copepods. Working solution was prepared with Qubit® protein reagent and Qubit® protein buffer (1:200). 190 µL working solution was pipetted into each well of a micro plate and 10 µL of sample or Qubit® protein standard (0, 200 and 400 ng/µL) was added. Solutions were mixed and incubated for 15 min at room temperature. Measurements were conducted with a micro plate reader (TriStar LB 941, Berthold Technologies) at 485 nm excitation and 590 nm emission, using the software MikroWin2000 (Berthold Technologies).
    Keywords: Basin Scale Analysis, Synthesis and Integration; Date/Time of event; D-MOC; Double opening/closing plankton net; EURO-BASIN; Event label; Latitude of event; Life stage; Longitude of event; Maria S. Merian; MSM26; MSM26_126-9; MSM26_127-17; MSM26_131-17; MSM26_133-6; MSM26_134-19; MSM26_135-16; MSM26_136-8; North Atlantic; Number of individuals; Proteins per individual; Sample ID; Taxon/taxa; TriStar LB 941 Microplate Reader (Berthold Technologies); Uniform resource locator/link to reference
    Type: Dataset
    Format: text/tab-separated-values, 126 data points
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  • 9
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    PANGAEA
    In:  Supplement to: Melle, Webjørn; Runge, Jeffrey A; Head, Erica J H; Plourde, Stéphane; Castellani, Claudia; Licandro, Priscilla; Pierson, James; Jónasdóttir, Sigrún Huld; Johnson, C; Broms, Cecilie; Debes, Høgni; Falkenhaug, Tone; Gaard, Eilif; Gislason, Astthor; Heath, Michael R; Niehoff, Barbara; Nielsen, Torkel Gissel; Pepin, Pierre; Stenevik, Erling Kaare; Chust, Guillem (2015): Biogeography of key mesozooplankton species in the North Atlantic and egg production of Calanus finmarchicus. Earth System Science Data, 7(2), 223-230, https://doi.org/10.5194/essd-7-223-2015
    Publication Date: 2024-05-28
    Description: Observations of egg production rates (EPR) for female Calanus finmarchicus were compared from different regions of the North Atlantic. The regions were diverse in size and sampling frequency, ranging from a fixed time series station in the Lower St Lawrence Estuary, off Rimouski, where nearly 200 experiments were carried out between May and December from 1994 to 2006, to a large-scale survey in the Northern Norwegian Sea, where about 50 experiments were carried out between April and June from 2002 to 2004. For this analysis the stations were grouped mostly along geographic lines, with only limited attention being paid to oceanographic features. There is some overlap between regions, however, where stations were sometimes kept together when they were sampled on the same cruise. As well some stations other than off Rimouski were occupied more than once during different years and/or in different seasons.
    Keywords: Area/locality; Basin Scale Analysis, Synthesis and Integration; Calanus finmarchicus, egg production rate, standard deviation; Calanus finmarchicus, egg production rate, standard error; Calanus finmarchicus, egg production rate per female; Calanus finmarchicus, eggs per clutch; Calanus finmarchicus, eggs per clutch, standard deviation; Calanus finmarchicus, eggs per clutch, standard error; Calanus finmarchicus, female, prosome length; Calanus finmarchicus, female, prosome length, standard deviation; Calanus finmarchicus, female, prosome length, standard error; Calanus finmarchicus, spawning frequency; Calanus finmarchicus, spawning frequency, standard deviation; Calanus finmarchicus, spawning frequency, standard error; Chlorophyll a, areal concentration; Chlorophyll a, standard deviation; Chlorophyll a, standard error; Depth, bottom/max; Depth, top/min; EURO-BASIN; LATITUDE; LONGITUDE; Number of measurements; Sampling date; Season; Temperature, water; Temperature, water, standard deviation; Temperature, water, standard error
    Type: Dataset
    Format: text/tab-separated-values, 1098 data points
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  • 10
    Publication Date: 2024-05-24
    Description: The activities of proteinases, lipases/esterases and citrate synthase of Calanus finmarchicus copepodites (CV) were analysed. Analysis was performed at 30°C for copepods from seven stations (126-9, 127-17, 131-17, 133-6, 134-19, 135-16, 136-8). In addition, thermal profiles (5-50°C) of these enzymes were analysed for copepods from 3 stations (127-17, 133-6, 135-16). C. finmarchicus of station 127-19 have been acclimated on board to two different temperatures (4 and 15°C) for two weeks. Thermal profiles (5-60°C) of lipases/esterases and proteinases of adult females from each treatment were analysed. Groups of 10 individuals were used to prepare enzyme extracts for analysis. From each station/treatment, three groups were analysed, each of which was measured in triplicates. The activity of proteinases was determined photometrically after Saborowski et al. (2004, hdl:10013/epic.20836), modified after Kreibich et al. (2008, doi:10.1007/s10152-008-0112-0). Azocasein was used as substrate. The lypolytic activity of lipases and esterases in the extract was analysed fluorometrically after Knotz et al. (2006, doi:10.1016/j.cbpa.2006.07.019) using 4-methylumbelliferyl butyrate as substrate. Citrate synthase activity was analysed photometrically after Stitt (1984) modified by Saborowski and Buchholz (2002) with oxaloacetic acid as substrate. For detailed description please contact the author.
    Keywords: Absorbance of control; Absorbance of sample; Basin Scale Analysis, Synthesis and Integration; Citrate synthase activity, dry mass; Citrate synthase activity per individual; Date; Date/Time of event; Depth, bottom/max; Depth, top/min; DEPTH, water; D-MOC; Double opening/closing plankton net; EURO-BASIN; Event label; Latitude of event; Life stage; Lipase activity, dry mass; Lipase activity per individual; Longitude of event; Maria S. Merian; MSM26; MSM26_126-9; MSM26_127-17; MSM26_127-19; MSM26_131-17; MSM26_133-6; MSM26_134-19; MSM26_135-16; MSM26_136-8; North Atlantic; Number of individuals; Proteinase activity, per dry mass, Delta extinction at 366 nm; Proteinase activity per individual, Delta extinction at 366 nm; Sample ID; Spectrophotometer Thermo Scientific UV1; Taxon/taxa; Treatment; Treatment: temperature; Uniform resource locator/link to reference; WP2; WP-2 towed closing plankton net
    Type: Dataset
    Format: text/tab-separated-values, 3564 data points
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