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Heritable genome editing in C. elegans via a CRISPR-Cas9 system (2013)

Ari E Friedland; Yonatan B Tzur; Kevin M Esvelt; Monica P Colaiácovo; George M Church; John A Calarco

Nature Publishing Group (NPG)

In: Nature Methods

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Publikationsdatum: 2013-07-31

Beschreibung: Nature Methods 10, 741 (2013). doi:10.1038/nmeth.2532 Authors: Ari E Friedland, Yonatan B Tzur, Kevin M Esvelt, Monica P Colaiácovo, George M Church & John A Calarco We report the use of clustered, regularly interspaced, short palindromic repeats (CRISPR)-associated endonuclease Cas9 to target genomic sequences in the Caenorhabditis elegans germ line using single-guide RNAs that are expressed from a U6 small nuclear RNA promoter. Our results demonstrate that targeted, heritable genetic alterations can be achieved in C. elegans, providing a convenient and effective approach for generating loss-of-function mutants.

Print ISSN: 1548-7091

Digitale ISSN: 1548-7105

Thema: Biologie , Medizin

Publiziert von Nature Publishing Group (NPG)

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