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  • 2010-2014  (6)
  • 2000-2004
  • 2012  (2)
  • 2011  (4)
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  • 2010-2014  (6)
  • 2000-2004
Year
  • 1
    Keywords: Hochschulschrift
    Type of Medium: Online Resource
    Pages: 1 Online-Ressource (87 Blatt = 1 MB) , Illustrationen, Diagramme
    Language: Undetermined
    Note: Zusammenfassung in deutscher und englischer Sprache
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  • 2
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    PANGAEA
    In:  Supplement to: Kreibich, Tobias; Saborowski, Reinhard; Hagen, Wilhelm; Niehoff, Barbara (2011): Influence of short-term nutritional variations on digestive enzyme and fatty acid patterns of the calanoid copepod Temora longicornis. Journal of Experimental Marine Biology and Ecology, 407(2), 182-189, https://doi.org/10.1016/j.jembe.2011.06.013
    Publication Date: 2023-10-28
    Description: Temora longicornis, a dominant calanoid copepod species in the North Sea, is characterised by low lipid reserves and high biomass turnover rates. To survive and reproduce successfully, this species needs continuous food supply and thus requires a highly flexible digestive system to exploit various food sources. Information on the capacity of digestive enzymes is scarce and therefore the aim of our study was to investigate the enzymatic capability to respond to quickly changing nutritional conditions. We conducted two feeding experiments with female T. longicornis from the southern North Sea off Helgoland. In the first experiment in 2005, we tested how digestive enzyme activities and enzyme patterns as revealed by substrate SDS-PAGE (sodium dodecylsulfate polyacrylamide gel electrophoresis) responded to changes in food composition. Females were incubated for three days fed ad libitum with either the heterotrophic dinoflagellate Oxyrrhis marina or the diatom Thalassiosira weissflogii. At the beginning and at the end of the experiment, copepods were deep-frozen for analyses. The lipolytic enzyme activity did not change over the course of the experiment but the enzyme patterns did, indicating a distinct diet-induced response. In a second experiment in 2008, we therefore focused on the enzyme patterns, testing how fast changes occur and whether feeding on the same algal species leads to similar patterns. In this experiment, we kept the females for 4 days at surplus food while changing the algal food species daily. At day 1, copepods were offered O. marina. On day 2, females received the cryptophycean Rhodomonas baltica followed by T. weissflogii on day 3. On day 4 copepods were again fed with O. marina. Each day, copepods were frozen for analysis by means of substrate SDS-PAGE. This showed that within 24 h new digestive enzymes appeared on the electrophoresis gels while others disappeared with the introduction of a new food species, and that the patterns were similar on day 1 and 4, when females were fed with O. marina. In addition, we monitored the fatty acid compositions of the copepods, and this indicated that specific algal fatty acids were quickly incorporated. With such short time lags between substrate availability and enzyme response, T. longicornis can successfully exploit short-term food sources and is thus well adapted to changes in food availability, as they often occur in its natural environment due seasonal variations in phyto- and microzooplankton distribution.
    Keywords: AWI; Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas; SPP1158
    Type: Dataset
    Format: application/zip, 2 datasets
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  • 3
    Publication Date: 2024-05-15
    Keywords: 6,9,12,15-Hexadecatetraenoic acid of total fatty acids; 6,9,12-Hexadecatrienoic acid of total fatty acids; 9,12-Hexadecadienoic acid of total fatty acids; all-cis-4,7,10,13,16,19-Docosahexaenoic acid of total fatty acids; all-cis-5,8,11,14,17-Eicosapentaenoic acid of total fatty acids; all-cis-6,9,12,15-Octadecatetraenoic acid of total fatty acids; all-cis-9,12,15-Octadecatrienoic acid of total fatty acids; Carbon/Nitrogen ratio; Carbon/Nitrogen ratio, standard deviation; Carbon content per individual; cis-11-Icosenoic acid of total fatty acids; cis-11-Octadecenoic acid of total fatty acids (IUPAC: Octadec-11-enoic acid); cis-15-Tetracosenoic acid of total fatty acids; cis-9-Hexadecenoic acid of total fatty acids (IUPAC: (9Z)-hexadec-9-enoic acid); cis-9-Octadecenoic acid of total fatty acids (IUPAC: Octadec-9-enoic acid); Dry mass, standard deviation; Dry mass per individual; Experiment day; Fatty acids, standard deviation; Hexadecanoic acid of total fatty acids; Lipids, standard deviation; Lipids per individual; Monounsaturated fatty acids of total fatty acids; Nitrogen content per individual; Octadecanoic acid of total fatty acids; Octadecatetraenoic acid 18:4(n-4) of total fatty acids; Polyunsaturated fatty acids of total fatty acids; Saturated fatty acids of total fatty acids; Sex; Species; Standard deviation; Tetradecanoic acid of total fatty acids; Treatment: food
    Type: Dataset
    Format: text/tab-separated-values, 225 data points
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  • 4
    Publication Date: 2024-05-15
    Keywords: 6,9,12-Hexadecatrienoic acid of total fatty acids; 9,12-Hexadecadienoic acid of total fatty acids; 9-Tetradecenoic acid of total fatty acids; all-cis-4,7,10,13,16,19-Docosahexaenoic acid of total fatty acids; all-cis-5,8,11,14,17-Eicosapentaenoic acid of total fatty acids; all-cis-6,9,12,15-Octadecatetraenoic acid of total fatty acids; all-cis-7,10,13,16,19-Docosapentaenoic acid of total fatty acids; all-cis-9,12,15-Octadecatrienoic acid of total fatty acids; all-cis-9,12-Octadecadienoic acid of total fatty acids; Carbon, organic, total; Carbon, organic, total, standard deviation; Carbon/Nitrogen ratio; Carbon/Nitrogen ratio, standard deviation; cis-11-Icosenoic acid of total fatty acids; cis-11-Octadecenoic acid of total fatty acids (IUPAC: Octadec-11-enoic acid); cis-13-Octadecenoic acid of total fatty acids; cis-7-Hexadecenoic acid of total fatty acids; cis-9-Hexadecenoic acid of total fatty acids (IUPAC: (9Z)-hexadec-9-enoic acid); cis-9-Octadecenoic acid of total fatty acids (IUPAC: Octadec-9-enoic acid); Fatty acids, standard deviation; Hexadecanoic acid of total fatty acids; Monounsaturated fatty acids of total fatty acids; Nitrogen, organic; Nitrogen, organic, standard deviation; Octadecanoic acid of total fatty acids; Octadecatetraenoic acid 18:4(n-4) of total fatty acids; Pentadecanoic acid of total fatty acids; Polyunsaturated fatty acids of total fatty acids; Sample type; Saturated fatty acids of total fatty acids; Tetradecanoic acid of total fatty acids
    Type: Dataset
    Format: text/tab-separated-values, 189 data points
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  • 5
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    WILEY-BLACKWELL PUBLISHING
    In:  EPIC3Journal of Applied Ichthyology, WILEY-BLACKWELL PUBLISHING, 28(5), pp. 756-765, ISSN: 0175-8659
    Publication Date: 2019-07-17
    Description: The aim of this study was to determine the macro-parasitic infestation level of oysters from the southern German Bight focussing on copepods of the genus Mytilicola. Crassostrea gigas, Ostrea edulis and Mytilus edulis were collected at five locations: three nearshore sites in the eastern Wadden Sea and two offshore cultivation sites in the German Bight. To reveal seasonal variations one sampling site was investigated in winter and summer. At the nearshore sites, Mytilicola orientalis was regularly detected in C. gigas. Prevalences ranged between 32.3% and 45.1%, intensity between 3.0 ± 0.6 and 8.2 ± 1.5. Infestation rates of C. gigas within the southern German Bight decreased from west to east: Apparently, M. orientalis has started its range extension along the German coast with gradual retardation eastwards but generally followed the invasion route of its main host, the Pacific oyster. Interestingly, we detected not only M. intestinalis but also M. orientalis as an intestinal parasite in M. edulis, which has sofar not previously been described as host within this region. We conclude that M. orientalis is flexible in its host choice. Furthermore, in the eastern Wadden Sea infestation rates of oysters and mussels by copepods are similar. These results deviate from the patterns observed for the northern Wadden Sea in terms of infestation level and host specificity. No macro-parasites were found in oysters and mussels from the offshore sites. This absence can be considered as potentially beneficial for aquaculture activities in the open ocean in terms of stamina and physiological performance.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , peerRev
    Format: application/pdf
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  • 6
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    In:  EPIC3XXXII SCAR and Open Science Conference, Portland, USA, 2012-07-16-2012-07-19
    Publication Date: 2019-07-17
    Description: Antarctic and Arctic zooplankton species have developed very similar life strategies and energetic adaptations to the harsh environment via sophisticated modes of lipid accumulation. A very efficient biosynthesis, storage and utilization of lipids enable especially herbivorous species to buffer the pronounced seasonality of food supply in the polar oceans. Lipid levels usually peak at the end of the productive season in autumn and reach minimum levels in spring. In many species lipid deposits are not primarily used for maintenance during winter but are conserved to fuel reproductive processes at the end of the dark season. The dependence on seasonal primary production is also reflected by the respective lipid compositions. Detailed lipid analyses of dominant Antarctic and Arctic copepods revealed that the herbivorous Calanus and Calanoides species have developed the most complex lipid biochemical pathways. They biosynthesise large amounts of wax esters with long-chain monounsaturated fatty acids and alcohols (20:1, 22:1) as major components. In contrast, the Antarctic Calanus propinquus and C. simillimus synthesise primarily triacylglycerols consisting mainly of long-chain monounsaturated fatty acids with 22 and even 24 carbon atoms (2 major isomers), which is very unusual among plankton species. In contrast, the lipids of omnivorous and carnivorous taxa such as Metridia or Euchaeta are deficient in such long-chain fatty acids and alcohols, although their lipid reserves mainly consist of wax esters. Our investigations underscore that lipids are a key factor in high latitude ecosystems, especially for the lower trophic levels. The extremely lipid-rich herbivorous species ensure an efficient lipid-based energy transfer and represent high-calory food for fish and warm-blooded animals like birds and mammals.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Conference , notRev
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