In:
PROTEOMICS, Wiley, Vol. 3, No. 12 ( 2003-12), p. 2402-2411
Kurzfassung:
Low levels and long term exposure to benzene is associated with hematotoxicity including aplastic anemia, acute myelogenous leukemia, and lymphoma. Current biomonitoring methods such as urinary phenol, S ‐phenylmercapturic acid, and trans‐trans muconic acid were found to be unreliable as analytical methods to detect benzene exposure. Therefore, to search for a specific protein for biomonitoring benzene exposure, we investigated plasma proteins from workers ( n = 50) at a printing company who were exposed to benzene, by two‐dimensional gel electrophoresis. The protein profiles are significantly different ( p 〈 0.05) between benzene exposed and unexposed groups, as identified by matrix‐assisted laser desorption ionization/time of flight mass spectrometry and confirmed by Western blot analyses. T cell receptor β chain (TCR β), FK506‐binding protein, and matrix metalloproteinase‐13 were expressed only in benzene exposed workers. In addition, interleukin‐4 receptor α chain and T cell surface glycoprotein CD1b precursor were found to be up‐regulated in the plasma of benzene exposed workers. When we treated Jurkat cells with benzene (10 μ M –10 m M ), TCR β expression was increased in the membrane more than 6–9‐fold compared to untreated cells. In addition, the amount of TCR β released into the culture media, at benzene concentrations greater than 50 μ M , increased up to 10 m M . Therefore, TCR β levels in plasma could be used as a biomarker and a possible therapeutic target for benzene exposure.
Materialart:
Online-Ressource
ISSN:
1615-9853
,
1615-9861
DOI:
10.1002/pmic.200300616
Sprache:
Englisch
Verlag:
Wiley
Publikationsdatum:
2003
ZDB Id:
2037674-1
SSG:
12
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