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  • Canadian Science Publishing  (9)
  • 2000-2004  (9)
  • 2001  (9)
  • 1
    Online Resource
    Online Resource
    Canadian Science Publishing ; 2001
    In:  Canadian Journal of Zoology Vol. 79, No. 3 ( 2001), p. 485-498
    In: Canadian Journal of Zoology, Canadian Science Publishing, Vol. 79, No. 3 ( 2001), p. 485-498
    Type of Medium: Online Resource
    ISSN: 1480-3283 , 0008-4301
    RVK:
    Language: Unknown
    Publisher: Canadian Science Publishing
    Publication Date: 2001
    detail.hit.zdb_id: 1490831-1
    SSG: 12
    Location Call Number Limitation Availability
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  • 2
    Online Resource
    Online Resource
    Canadian Science Publishing ; 2001
    In:  Canadian Journal of Zoology Vol. 79, No. 3 ( 2001-03-01), p. 485-498
    In: Canadian Journal of Zoology, Canadian Science Publishing, Vol. 79, No. 3 ( 2001-03-01), p. 485-498
    Abstract: We present morphological data on the four subspecies of painted turtles (Chrysemys picta) from 20 states in the U.S.A. and three provinces in Canada, and from localities within them that are removed from reported zones of intergradation. The characters used are those of Hartman (1958), which have been routinely used in subsequent studies: the extent of plastral patterning (here quantified by image analysis), the degree of misalignment of carapacial scutes, and the width of the light anterior margins of the second lateral scutes. Our purposes are to present descriptive baseline data for each subspecies for use in comparisons with the results of past and future studies of intergradation, and to critique the oft-accepted theory of Bleakney (1958) regarding the evolutionary origins of the current subspecies, particularly C. p. marginata. Data are given for C. p. bellii from 5 states ranging from Minnesota to New Mexico, for C. p. dorsalis from 3 states (Arkansas, Louisiana, and Mississippi), for C. p. marginata from 4 localities (Ontario, Michigan, Indiana, and Ohio), and for C. p. picta from 11 localities from Nova Scotia to Georgia. We find that (i) a "pure" picta does not exist; there is marginata influence throughout picta's range, particularly at the extremes; (ii) similarities exist that group dorsalis with picta; (iii) there is a north-south cline of reduced scute alignment in picta; (iv) there is a north-south cline of increasing extent of the plastral pattern in bellii; (v) the question of which subspecies first reached New England after the Wisconsinan glaciation remains unresolved, but it was likely not picta; (vi) there is no compelling reason to accept or reject Bleakney's theory of the distribution of painted turtle subspecies at the height of the Wisconsinan glaciation or his conjecture that marginata arose as in intergrade of bellii and dorsalis; in fact, it could be argued that if marginata is a hybrid, it could be a hybrid of picta and bellii.
    Type of Medium: Online Resource
    ISSN: 0008-4301 , 1480-3283
    RVK:
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 2001
    detail.hit.zdb_id: 1490831-1
    SSG: 12
    Location Call Number Limitation Availability
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  • 3
    Online Resource
    Online Resource
    Canadian Science Publishing ; 2001
    In:  Canadian Journal of Microbiology Vol. 47, No. 4 ( 2001-04-01), p. 368-372
    In: Canadian Journal of Microbiology, Canadian Science Publishing, Vol. 47, No. 4 ( 2001-04-01), p. 368-372
    Abstract: It was previously proposed that plant growth-promoting bacteria that possess 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase could utilize ACC that is present in the exudate of germinating canola seeds. The uptake and cleavage of ACC by these bacteria would lower the level of ACC, and thus ethylene within the plant, and reduce the extent of its inhibition on root elongation. To test part of the above mentioned model, ACC levels were monitored in canola seed tissues and exudate during germination. Lower amounts of ACC were present in the exudate and tissues of seeds treated with the plant growth-promoting bacterium Enterobacter cloacae CAL3, than in control seeds treated with MgSO 4 . The ACC-related compounds, α- and γ-aminobutyric acids, both known to stimulate ethylene production, were also measured in the canola seed exudate and tissues. Approximately the same levels of α-aminobutyric acid were present in the exudates of the bacterium-treated seeds and the control seeds, but the amount of α-aminobutyric acid was lower in the tissues of the bacterium-treated seeds than in the control seeds. Smaller quantities of γ-aminobutyric acid were seen in both the exudate and tissues of the E. cloacae CAL3-treated seeds than in the control seeds.Key words: ACC ethylene, canola, seed extract, seed exudate, plant growth-promoting bacteria.
    Type of Medium: Online Resource
    ISSN: 0008-4166 , 1480-3275
    RVK:
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 2001
    detail.hit.zdb_id: 280534-0
    detail.hit.zdb_id: 1481972-7
    SSG: 12
    Location Call Number Limitation Availability
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  • 4
    Online Resource
    Online Resource
    Canadian Science Publishing ; 2001
    In:  Canadian Journal of Microbiology Vol. 47, No. 4 ( 2001), p. 359-367
    In: Canadian Journal of Microbiology, Canadian Science Publishing, Vol. 47, No. 4 ( 2001), p. 359-367
    Type of Medium: Online Resource
    ISSN: 1480-3275 , 0008-4166
    RVK:
    Language: Unknown
    Publisher: Canadian Science Publishing
    Publication Date: 2001
    detail.hit.zdb_id: 280534-0
    detail.hit.zdb_id: 1481972-7
    SSG: 12
    Location Call Number Limitation Availability
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  • 5
    Online Resource
    Online Resource
    Canadian Science Publishing ; 2001
    In:  Canadian Journal of Forest Research Vol. 31, No. 5 ( 2001-05-01), p. 865-878
    In: Canadian Journal of Forest Research, Canadian Science Publishing, Vol. 31, No. 5 ( 2001-05-01), p. 865-878
    Abstract: Two procedures that guarantee the property of additivity among the components of tree biomass and total tree biomass utilizing nonlinear functions are developed. Procedure 1 is a simple combination approach, and procedure 2 is based on nonlinear joint-generalized regression (nonlinear seemingly unrelated regressions) with parameter restrictions. Statistical theory is given for construction of confidence and prediction intervals for the two procedures. Specific examples using slash pine (Pinus elliottii Engelm. var. elliottii) biomass data are presented to demonstrate and clarify the methods behind nonlinear estimation, additivity, error modeling, and the formation of confidence and prediction intervals. Theoretical considerations and empirical evidence indicate procedure 2 is generally superior to procedure 1. It is argued that modeling the error structure is preferable to using the logarithmic transformation to deal with the problem of heteroscedasticity. The techniques given are applicable to any quantity that can be disaggregated into logical components.
    Type of Medium: Online Resource
    ISSN: 0045-5067 , 1208-6037
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 2001
    detail.hit.zdb_id: 1473096-0
    SSG: 23
    SSG: 12
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  • 6
    Online Resource
    Online Resource
    Canadian Science Publishing ; 2001
    In:  Canadian Journal of Microbiology Vol. 47, No. 1 ( 2001-01-01), p. 77-80
    In: Canadian Journal of Microbiology, Canadian Science Publishing, Vol. 47, No. 1 ( 2001-01-01), p. 77-80
    Abstract: Previously, it was proposed that plant growth-promoting bacteria that possess the enzyme, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, can reduce the amount of ethylene produced by a plant and thereby promote root elongation. To test this model, canola seeds were imbibed in the presence of the chemical ethylene inhibitor, 2-aminoethoxyvinyl glycine (AVG), various strains of plant growth-promoting bacteria, and a psychrophilic bacterium containing an ACC deaminase gene on a broad host range plasmid. The extent of root elongation and levels of ACC, the immediate precursor of ethylene, were measured in the canola seedling roots. A modification of the Waters AccQ*Tag Amino Acid Analysis Method(tm) was used to quantify ACC in the root extracts. It was found that, in the presence of the ethylene inhibitor, AVG, or any one of several ACC deaminase-containing strains of bacteria, the growth of canola seedling roots was enhanced and the ACC levels in these roots were lowered.
    Type of Medium: Online Resource
    ISSN: 0008-4166 , 1480-3275
    RVK:
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 2001
    detail.hit.zdb_id: 280534-0
    detail.hit.zdb_id: 1481972-7
    SSG: 12
    Location Call Number Limitation Availability
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  • 7
    Online Resource
    Online Resource
    Canadian Science Publishing ; 2001
    In:  Canadian Journal of Microbiology Vol. 47, No. 4 ( 2001-04-01), p. 359-367
    In: Canadian Journal of Microbiology, Canadian Science Publishing, Vol. 47, No. 4 ( 2001-04-01), p. 359-367
    Abstract: Based on DNA sequence analysis and 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity, the region of DNA immediately upstream of the Enterobacter cloacae UW4 ACC deaminase gene (acdS) contains several features that appear to be involved in its transcriptional regulation. In the present study, the 5' upstream region of acdS was cloned into the promoter-probe vector, pQF70, which carries the promoterless luciferase gene (luxAB), and luciferase expression was monitored. The data obtained from studying the expression of the luciferase gene showed that (i) a leucine responsive regulatory protein (LRP)-like protein encoded within the upstream region is located on the opposite strand from acdS under the control of a promoter stronger than the one responsible for acdS transcription, (ii) luciferase gene expression required both ACC and the LRP-like protein, (iii) luciferase expression was increased three-fold under anaerobic conditions, consistent with the involvement of a fumarate-nitrate reduction (FNR)-like regulatory protein box within the upstream region, and (iv) the addition of leucine to the growth medium decreased luciferase activity in the presence of ACC and increased luciferase activity in the absence of ACC, consistent with leucine acting as a regulator of the expression of the LRP-like protein.Key words: plant growth promotion, ethylene, ACC deaminase, regulation, Enterobacter cloacae.
    Type of Medium: Online Resource
    ISSN: 0008-4166 , 1480-3275
    RVK:
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 2001
    detail.hit.zdb_id: 280534-0
    detail.hit.zdb_id: 1481972-7
    SSG: 12
    Location Call Number Limitation Availability
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  • 8
    Online Resource
    Online Resource
    Canadian Science Publishing ; 2001
    In:  Canadian Journal of Microbiology Vol. 47, No. 8 ( 2001-08-01), p. 698-705
    In: Canadian Journal of Microbiology, Canadian Science Publishing, Vol. 47, No. 8 ( 2001-08-01), p. 698-705
    Abstract: The plant growth-promoting bacteria Enterobacter cloacae CAL2 and UW4 were genetically transformed with a multicopy plasmid containing an rpoS or gacS gene from Pseudomonas fluorescens. The transformed strains were compared with the nontransformed strains for growth, indoleacetic acid (IAA) production, antibiotic production, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity, siderophore production, cell morphology, and the ability to promote canola root elongation. All transformed strains had a longer lag phase, were slower in reaching stationary phase, and attained a higher cell density than the nontransformed strains. Transformation resulted in cells that were significantly shorter than the nontransformed cells. The transformed strains also produced significantly more IAA than the nontransformed strains. Introduction of rpoS or gacS from Pseudomonas fluorescens was associated with a reduction in the production of both antibiotics, 2,4-diacetylphloroglucinol and mono-acetylphloroglucinol, produced by Enterobacter cloacae CAL2. With Enterobacter cloacae CAL2, plasmid-borne rpoS, but not gacS, increased the level of ACC deaminase activity, while introduction of rpoS in Enterobacter cloacae UW4 caused a decrease in ACC deaminase activity. Neither gacS nor rpoS significantly affected the level of siderophores synthesized by either bacterial strain. Overproduction of either GacA or RpoS in Enterobacter cloacae CAL2 resulted in a significant increase in the root lengths of canola seedlings when seeds were treated with the bacteria, and overproduction of RpoS caused an increase in canola shoot as well as root lengths.Key words: plant growth-promoting bacteria, canola, ethylene, ACC deaminase, GacS, RpoS, indoleacetic acid, siderophores, antibiotics.
    Type of Medium: Online Resource
    ISSN: 0008-4166 , 1480-3275
    RVK:
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 2001
    detail.hit.zdb_id: 280534-0
    detail.hit.zdb_id: 1481972-7
    SSG: 12
    Location Call Number Limitation Availability
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  • 9
    Online Resource
    Online Resource
    Canadian Science Publishing ; 2001
    In:  Canadian Journal of Microbiology Vol. 47, No. 8 ( 2001), p. 698-705
    In: Canadian Journal of Microbiology, Canadian Science Publishing, Vol. 47, No. 8 ( 2001), p. 698-705
    Type of Medium: Online Resource
    ISSN: 1480-3275 , 0008-4166
    RVK:
    Language: Unknown
    Publisher: Canadian Science Publishing
    Publication Date: 2001
    detail.hit.zdb_id: 280534-0
    detail.hit.zdb_id: 1481972-7
    SSG: 12
    Location Call Number Limitation Availability
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