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  • 1995-1999  (5)
  • 1999  (5)
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  • 1995-1999  (5)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Occurrence of ganglioside GD3 in neoplastic astrocytes (1999)
    Springer
    Virchows Archiv 434 (1999), S. 201-205 
    Details
    ISSN: 1432-2307
    Keywords: Key words Ganglioside GD3 ; Glioblastoma multiforme ; Astrocytoma ; Glioma ; Immunocytochemistry ; Thin-layer chromatography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  GD3 immunocytochemical analysis was performed in 25 human specimens obtained by autopsy and biopsy from patients with astrocytomas, anaplastic astrocytomas, cerebellar astrocytomas and glioblastoma multiforme (GM), using the ABC method. Extraction of the ganglioside fraction from GM was used for thin-layer chromatography (TLC) analysis to confirm the specificity of anti-GD3 monoclonal antibody (DSG-1). Normal astrocytes were not immunoreactive for GD3. Neoplastic astrocytes of low- to high-grade tumours were GD3 immunoreactive. In GM, the multinucleated giant cells were also immunoreactive. All immunoreactivity present was within the cytoplasm. In TLC analysis, enzyme immunostaining of gangliosides from GM with DSG-1 showed only one positive band, which had the same TLC migration rate as GD3, indicating that GD3 of the ganglioside fraction from GM is the antigen detected by DSG-1. The presence of GD3 within the cytoplasm of neoplastic astrocytes showing invasive and proliferative properties, is of considerable interest. The implications and possible significance of the presence of GD3 in the cytoplasm in glioma cells are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004280050328
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  • 2
    Electronic Resource
    Electronic Resource
    Lactoferrin Concentration in Milk of Bovine Clinical Mastitis (1999)
    Springer
    Veterinary research communications 23 (1999), S. 391-398 
    Details
    ISSN: 1573-7446
    Keywords: aetiology ; cow ; lactoferrin ; mastitis ; non-specific immunity ; pathogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The lactoferrin (LF) concentration in the milk from dairy cows with clinical mastitis was determined to evaluate the relationship between the LF concentration (LFC) in milk and the non-specific defensive capability of the udder. The mean LFC in 368 milk samples from 319 cows with clinical mastitis was significantly higher (p〈0.01) than that of normal cows. The mean LFC in milk from quarters infected with Mycoplasma bovis or Staphylococcus aureus was significantly higher (p〈0.05) than that of quarters infected with coagulase-negative staphylococci (CNS). In Escherichia coli mastitis, the level of LFC in milk from cows with peracute mastitis was significantly lower (p〈0.01) than that from cows with acute mastitis. In cases of mastitis due to E. coli, the mean LFC in milk from cows that needed more than 10 days to recover from the mastitis or were not cured was significantly lower (p〈0.05) than that for cows which took less than 10 days to be cured. The mean LFC in milk from cows with peracute E. coli mastitis was significantly lower (p〈0.05) than that for cows with mastitis associated with environmental streptococci or CNS, although these low LF levels were somewhat increased after 46 h from the occurrence of mastitis. These results suggest that the decreased levels of LF in peracute E. coli mastitis may be associated with the progress of inflammation in the early phase of mastitis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006347423426
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  • 3
    Electronic Resource
    Electronic Resource
    Adrenal Adenoma with Foci of Oncocytes in a Patient with Pre-Cushing's Syndrome (1999)
    Springer
    International urology and nephrology 31 (1999), S. 1-6 
    Details
    ISSN: 1573-2584
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A case of unilateral adrenal adenoma with foci of oncocytes in a 64-year-old woman is reported. The tumour manifested clinically pre-Cushing's syndrome with autonomous steroidogenesis which was insufficient to cause typical features of Cushing's syndrome. Whether the pathogenesis of oncocytes and adenoma cells, which were thought to have some metabolic deficiency in the present tumour, are linked remains to be determined.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007168819539
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  • 4
    Electronic Resource
    Electronic Resource
    Cell-killing efficiency and number of platinum atoms binding to DNA, RNA and protein molecules of HeLa cells treated with combinations of hyperthermia and carboplatin (1999)
    Springer
    Journal of radioanalytical and nuclear chemistry 239 (1999), S. 405-409 
    Details
    ISSN: 1588-2780
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract The effect of hyperthermia on the cell killing efficiency of Pt atoms binding to DNA, RNA and protein molecules of HeLa cells treated withcis-diamine(1,1-cyclobutanedicarboxylato)platinum(II) (CBDCA) was examined. HeLa S-3 cells were treated with195mPt-radiolabeled CBDCA for 60 minutes at various temperatures, and the relationship between the lethal effect and the number of Pt atoms binding to DNA, RNA and proteins was examined. The mean lethal concentration (D0) of carboplatin for a 60 min-treatment at 0, 25, 37, 40, 42 and 44°C was 671.2, 201.5, 67.3, 33.4, 20.2 and 15.6 μM, respectively. By using identically treated cells, the number of Pt-atoms combined with DNA, RNA and protein molecules were determined in the subcellular fractions. Thus, the D0's given as the drug concentrations were replaced with the number of Pt-atoms combined in each fraction. Then, the cell-killing efficiency of the Pt atom was expressed as the reciprocal of the number of Pt-atoms combined and was calculated for each molecule. The efficiency for DNA molecules was 0.699, 1.42, 2.65, 4.84, 7.74 and 8.28·104 nucleotides, respectively, for the conditions described above. From 0 to 44°C, the cell-killing efficiency of Pt atoms increased by a factor of 11.9.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02349521
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  • 5
    Electronic Resource
    Electronic Resource
    Enhanced cell-killing effect of potassium hexachloroplatinum(IV) solution irradiated by60Co γ-rays under the presence of NH4Cl (1999)
    Springer
    Journal of radioanalytical and nuclear chemistry 239 (1999), S. 401-404 
    Details
    ISSN: 1588-2780
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract The enhancement of cell-killing effect induced by60Co γ-rays on the aqueous solutions of potassium hexachloroplatinum(IV) (K2PtCl6) irradiated in the presence of various concentrations of NH4Cl was examined. HeLa S-3 cells were treated with the irradiated solutions for 60 minutes at 37°C, and cell survival was measured by colony forming efficiency. The mean lethal concentration (D 0) for the cells treated with non-irradiated K2PtCl6 solution (1 mg/ml) was 34.5 μl/ml. However, it decreased, namely the cell-killing effect of the solution increased with increasing radiation dose up to 2.64·104 Gy in the case of 0.1M NH4Cl solution (in this condition, theD 0 decreased to 9.6 μl/ml). No or only a slight enhancement was observed in the case of K2PtCl6 solutions irradiated in the presence of NaCl, (NH4)2HPO4, ammonium acetate or glycine. Observations using high performance liquid chromatography (HPLC) of irradiated K2PtCl6 solutions revealed the formation of cis- and/or trans-diaminedichloroplatinum (IV) (DDPs(IV)). However, the observed cell-killing action of the solution was too high to be explained by the amount of both the DDPs(IV) formed by γ-rays.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02349520
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