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  • 1990-1994  (2)
  • 1993  (2)
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  • 1990-1994  (2)
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  • 1
    ISSN: 1573-0603
    Keywords: in vitro ; fluorescent dyes ; cell culture ; cytotoxicity ; L929
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A semi-quantitative procedure is described for measuring cell viability after short-term exposure to a test substance using a monolayer culture. Test substances are placed in direct contact with cell monolayers for various time intervals. The substances are removed and the monolayers are incubated in the presence of fluorescein diacetate. Monolayers are viewed under a fluorescent microscope and the percentage of fluorescing (viable) cells is estimated. The method is suitable for examining cytotoxic effects at short times of exposure and for discriminating between test substances that give similar, low toxicity endpoints in standard 24-h assays.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Hydrobiologia 269-270 (1993), S. 197-205 
    ISSN: 1573-5117
    Keywords: Pseudonitzschia ; domoic acid ; amnesic shellfish poisoning ; razor clams ; Dungeness crabs ; N.E. Pacific Ocean
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Members of the planktonic diatom genus Pseudonitzschia H. Peragallo, P. pungens (Grun.) Hasle f. multiseries (Hasle) Hasle and P. australis Frenguelli, that may produce the marine biotoxin, domoic acid, have been recognized in western Washington waters. Their distribution is not well-known in this area, probably because they often have been misidentified. However, they appear to be relatively common and may be abundant, especially in late spring and summer. Domoic acid, a potent neurotoxin, was found at levels up to 154 µg g−1 wet weight in razor clams, Siliqua patula Dixon, and Dungeness crabs, Cancer magister Dana, on the Pacific coast of Washington in late October 1991. It was also found in trace amounts in blue mussels, Mytilus edulis L., and oysters, Crassostrea gigas Thun., in the inland waters of northern Puget Sound in summer 1992. The presence of these potentially toxic diatoms signals the need for regular phytoplankton monitoring and additional shellfish monitoring to ensure that seafood is safe for human consumption. Further, studies are needed on the physiological ecology of the diatoms to determine the causative factors leading to production of the toxin.
    Type of Medium: Electronic Resource
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