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  • 1990-1994  (4)
  • 1960-1964
  • 1993  (4)
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  • 1990-1994  (4)
  • 1960-1964
Year
  • 1
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: A comparison of the pharmacological and physiological properties of the metabotropic glutamate 1α and 1β receptors (mGluR1α and mGluR1β) expressed in baby hamster kidney (BHK 570) cells was performed. The mGluR1β receptor is an alternatively spliced form of mGluR1α with a modified carboxy terminus. Immunoblots of membranes from the two cell lines probed with receptor-specific antipeptide antibodies showed that mGluRIa migrated with an Mr= 154, 000, whereas mGluR1β migrated with an Mr= 96, 000. Immunofluorescence imaging of receptors expressed in BHK 570 cells revealed that the mGluR1α receptor was localized to patches along the plasmalemma and on intracellular membranes surrounding the nucleus, whereas mGluR1β was distributed diffusely throughout the cell. Agonist activation of the mGluR1α and the mGluR1β receptors stimulated phosphoinositide hydrolysis. At both receptors, glutamate, quisqualate, and ibotenate were full agonists, whereas trans-(+)-1-aminocyclopentane-1, 3-dicarboxylate appeared to act as a partial agonist. The stimulation of phosphoinositide hydrolysis by mGluR1α showed pertussis toxin-sensitive and insensitive components, whereas the mGluR1β response displayed only the toxin-insensitive component. The mGluR1α and mGluR1β receptors also increased intracellular calcium levels by inducing release from intracellular stores. These results indicate that the different carboxy terminal sequences of the two receptors directly influences G protein coupling and subcellular deposition of the receptor polypeptides and suggest that the two receptors may subserve different roles in the nervous system.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Applied physics 57 (1993), S. 299-302 
    ISSN: 1432-0630
    Keywords: 33.00 ; 35.00 ; 78.30
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract The resonant Raman scattering of C60 crystallites is monitored as a function of excitation intensity at 514.5 nm. At low intensities, a strong line at 1468 cm−1 is observed. No feature at 1459 cm−1 is observable. With increasing intensities, the 1468 cm−1 line shifts continuously and reversibly to lower frequencies. The mode softening is nonlinearly dependent on the input intensity and is accompanied by a nonlinear increase in the Raman intensity. The spectral changes are discussed in terms of a nonlinear reduction in force constant and increase in bond polarisability as a result of an increased intermolecular delocalisation of the π-electron cloud. The nonlinear changes are associated with the nonlinear luminescence and photoconductive response observed in fullerenes and are contrasted to the irreversible phototransformation recently discussed.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-1084
    Keywords: Magnetic resonance imaging (MRI) ; Flow-quantification ; Portal vein ; Standardized meal
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Portal venous blood flow was measured with a double-oblique phase-sensitive magnetic resonance imaging (MRI) flow measurement technique in six healthy volunteers after 7 h of fasting. A standard liquid meal with an energy content of 4200 kJ, containing equal parts of carbohydrate, protein and fat was given to the volunteers while in the scanner. Flow measurements were performed 5, 15 and 30 min after intake of the meal. The average portal vein blood flow was 0.88 ± 0.17 l · min−1 during fasting. After intake of the standard meal, flow increased by 30,67 and 111% after 5, 15 and 30 min, respectively. This study shows that a non-invasive MRI technique can be used for volume flow determinations in the portal vein and that physiological changes in flow can be quantified. The technique may be of clinical value in evaluation of patients with abdominal angina as well as patients with portal hypertension.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1434-6036
    Keywords: 74.25.Gz ; 74.25.Kc ; 78.30.Er
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract We report a far-infrared reflectivity study of DyBa2Cu4O8, HoBa2Cu4O8 and Y2Ba4Cu7O15−δ superconductors in the temperature range 10–300 K. The assignment of thez-polarized infrared-acitve vibrations of the rare-earth-ion (R) was verified by the substitution ofR. Since the structure of the Y2Ba4Cu7O15−δ compound inz-direction is an ordered sequence of YBa2Cu3O7−δ and YBa2Cu4O8 blocks, the phonon spectrum of Y2Ba4Cu7O15−δ is a superposition of their respective phonon modes. The temperature dependence of the infrared-active phonons in RBa2Cu4O8 and Y2Ba4Cu7O15−δ was analyzed. A narrowing of the plane-oxygen phonon upon sample cooling was found to occur atT c , as it was reported for single-chain YBa2Cu3O7 materials; however a pronounced frequency softening of this mode for both RBa2Cu4O8 and Y2Ba4Cu7O15−δ was found to start well aboveT c , suggesting the interaction of infrared-active phonons with an excitation gap similar to that observed in spin-dependent measurements.
    Type of Medium: Electronic Resource
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