ISSN:
1432-136X
Keywords:
Mitochondrial sulfide detoxification
;
Fundulus parvipinnis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary Earlier whole-animal experiments have shown that the California killifish (Fundulus parvipinnis) from tidal marshes is highly tolerant to sulfide while the speckled sanddab (Citharichtys stigmaeus) from the open coast is intolerant to sulfide. In the present paper, we demonstrate that the liver mitochondria of the California killifish detoxify sulfide by oxidizing it to thiosulfate and produce ATP in the process. Sulfide oxidation is obligately and stoichiometrically linked to mitochondrial electron transport to oxygen. Concentrations up to 20 μM sulfide stimulate mitochondrial respiration while 50 μM sulfide causes half-inhibition. Sulfide oxidation by mitochondria is adversely affected at pH〈7.4. ATP production is maximal at 10 μM sulfide. The finding of sulfide oxidation coupled to ATP production by killifish mitochondria is unprecedented among vertebrates. In comparison, mitochondria of the specked sanddab oxidize sulfide at a much lower rate. This is the first demonstration of biochemical adaptation to sulfide among coastal marine fishes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00258979
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