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  • Springer  (5)
  • 1995-1999
  • 1990-1994  (5)
  • 1990  (5)
Document type
Keywords
Publisher
  • Springer  (5)
Years
  • 1995-1999
  • 1990-1994  (5)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Simple repetitive sequences are associated with differentiation of the sex chromosomes in the guppy fish (1990)
    Springer
    Journal of molecular evolution 30 (1990), S. 456-462 
    Details
    ISSN: 1432-1432
    Keywords: Chromosomal sex determination ; Heterochromatin ; “Junk” DNA ; DNA fingerprinting ; Oligonucleotide hydridization in situ
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Hybridization of restriction enzymedigested genomic guppy (Poecilia reticulata, Poeciliidae) DNA with the oligonucleotide probe (GACA)4 revealed a male-specific simple tandem repeat locus, which defines the Y chromosome in outbred populations. The related (GATA)4 probe identifies certain males with the red color phenotype. In contrast only in two out of eight laboratory guppy strains was the typical (GACA)4 band observed. By specific staining of the constitutive heterochromatin one pair of chromosomes could also be identified as the sex chromosomes, confirming the XX/XY mechanism of sex determination. All males exhibit Y chromosomes with a large region of telomeric heterochromatin. Hybridization in situ with nonradioactively labeled oligonucleotide probes localized the (GACA)n repeats to this heterochromatic portion. Together these results may be regarded as a recent paradigm for the differentiation of heteromorphic sex chromosomes from a pair of autosomes during the course of evolution. According to the fish model system, this may have happened in several independent consecutive steps.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02101117
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Y isochromosome associated with a mosaic karyotype and inactivation of the centromere (1990)
    Springer
    Human genetics 〈Berlin〉 85 (1990), S. 486-490 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A patient with azoospermia and a Y isochromosome is described. The breakpoint producing this i(Y) was within the terminal short arm of the Y chromosome. Lymphocyte cultures from peripheral blood contained a high proportion of 45,X cells and cells with different Y-chromosome rearrangements. The i(Y) had either a monocentric or dicentric appearance. In dicentrics, anti-kinetochore immunofluorescence was present at both centromeres. However, this was also true for most of the functional monocentrics (pseudodicentrics). Kinetochore staining was generally positive at the site of the inactive centromeres; only a minority of the suppressed centromeres had lost their antigenic properties. Permanently growing lymphoblasts consistently showed a monocentric i(Y) with only one fluorescing kinetochore; the immunonegative Y centromere did not recover antigenicity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00194221
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Heterogeneities in the distribution of (GACA)n simple repeats in the karyotypes of primates and mouse (1990)
    Springer
    Human genetics 〈Berlin〉 85 (1990), S. 187-194 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Tandemly organized simple repetitive sequences are widespread in all eukaryotes. The organization of the simple tetrameric (GACA)n sequences at chromosomal loci has been investigated using in situ hybridization with chemically pure oligonucleotide probes. Both biotin- and digoxigenin-attached (GACA)4 probes reveal specific hybridization signals over the short arms of all acrocentric autosomes in man. In the other examined primates the NOR-bearing autosomes could be detected by in situ hybridization with (GACA)4, and a major concentration of the GACA simple repeats could be observed on the Y chromosome in the gibbon and mouse; the hybridization site in the gibbon Y chromosome coincides particularly with the silver-stainable NOR. In the past, accumulations of (GACA)n sequences were demonstrated mainly on vertebrate sex chromosomes. Therefore, the organization of GACA simple sequences is discussed in the context of their evolutionary potential accumulation and the possible linkage with the primate rDNA loci.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00193194
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Kinetochore formation in experimentally undercondensed chromosomes (1990)
    Springer
    Human genetics 〈Berlin〉 84 (1990), S. 535-538 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Treatment of human and mouse cell cultures with the cytidine analogue 5-azadeoxycytidine and the AT-specific DNA ligand Hoechst 33258 dramatically inhibited condensation of the pericentromeric heterochromatin in several chromosomes. When stained with antikinetochore autoimmune sera, these experimentally undercondensed chromosomes showed kinetochores with preserved antigenicity. The undercondensed and normally condensed chromosomes share the major antigenic determinants of the kinetochore.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00210805
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Cytogenetic and molecular analysis of a Yq isochromosome (1990)
    Springer
    Human genetics 〈Berlin〉 86 (1990), S. 147-150 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The dicentric Yq isochromosome of a male with azoospermia and some features of Klinefelter's syndrome was examined using cytogenetic and molecular methods. C- and R-banding of chromosomes of peripheral blood lymphocytes revealed a complex mosaic consisting of 46,X,i(Yq)/45,XO/46.XY/47,XYY/47,XY, i(Yq) / 47,X,i(Yq),i(Yq) cells. EBV-transformed lymphocytes either had a 46,X,i(Yq) (90%) or a 46,X,+mar (10%) karyotype. The marker chromosome was shown to be Y-derived by in situ hybridization. C-banding, quinacrine-and DA/DAPI-staining indicated inactivation of one of the centromeres in almost all Yq isochromosomes. The use of Y chromosomal DNA sequences demonstrated that most of the Y chromosome, including its short arm, was duplicated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00197696
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    Paper (German National Licenses)
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