ISSN:
1432-2048
Keywords:
Calmodulin-stimulated ATPase
;
Plasma membrane (Ca2+ transport)
;
Zea (Ca2+ transport)
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The calmodulin-stimulated ATPase of maize (Zea mays L.) coleoptiles has been purified by calcium-dependent binding to a calmodulin affinity column. In the presence of protease inhibitors (phenylmethylsulfonylfluoride and chymostatin) a polypeptide of relative molecular mass (Mr) 140000 (±10000) is obtained on sodium-dodecylsulphate polyacrylamide gels. This polypeptide is recognised specifically by an affinity-purified polyclonal antibody to mammalian calmodulin-stimulated calcium-pumping ATPases and is of similar Mr to the erythrocyte-membrane calcium pump (138000 Mr).
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00392457
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