In:
Genetical Research, Hindawi Limited, Vol. 50, No. 2 ( 1987-10), p. 113-123
Abstract:
Sex determination in Drosophila depends on the ratio of X chromosomes to sets of autosomes (X:A). This chromosomal signal is used to regulate a few control genes whose state of activity selects either the male or the female sexual pathway. We have studied the structure and function of dsx (double sex) which appears to be the last regulatory gene on whose function the sexual pathway eventually depends. We have mutagenized the locus, varied the doses of dominant dsx -mutations and wildtype alleles, and combined different dsx -alleles with recessive mutations in other sex-determining genes, such as ix , tra-2 and tra . The locus dsx harbours two genetic functions, dsx m to implement the male program, dsx f to implement the female program. We found that dsx m and dsx f can mutate independently although most mutations abolish both functions. We conclude that dsx m and dsx f each have their specific domain, but also share a large region of DNA that is essential for both functions. We present evidence that the dominant mutations correspond to a constitutive expression of the male-determining function dsx m , with the simultaneous abolishment of the female-determining function dsx f . This effect can be counteracted by two doses of expressed dsx f so that a female phenotype results. The products of one dose of expressed dsx m and one dose of expressed dsx f in the same cell appear to neutralize each other which leads to a null phenotype. The mutant combinations suggest that the product of dsx f requires the products of ix + , tra -2 + and tra + to become functional.
Type of Medium:
Online Resource
ISSN:
0016-6723
,
1469-5073
DOI:
10.1017/S001667230002351X
Language:
English
Publisher:
Hindawi Limited
Publication Date:
1987
detail.hit.zdb_id:
2412684-6
detail.hit.zdb_id:
1472156-9
SSG:
12
Permalink
