Electronic Resource
Oxford, UK
:
Blackwell Publishing Ltd
FEMS microbiology letters
30 (1985), S. 0
ISSN:
1574-6968
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
Abstract Cell extracts of toluene-grown Pseudomonas putida produced a soluble yellow dye during aerobic incubations with indole and NADH. Accumulation of indoxyl in reaction mixtures corresponded with a linear increase in absorbance at 400 nm. The rate of increase in absorbance was shown to be a specific measure of toluene dioxygenase activity. The primary product of toluene oxidation, cis-toluene dihydrodiol, inhibited dioxygenase activity in cell extracts containing no detectable activity of cis-toluene dihydrodiol dehydrogenase.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1574-6968.1985.tb01017.x
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