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  • 2020-2023
  • 1990-1994  (4)
  • 1975-1979
  • 1991  (4)
  • 1984
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  • 1
    Electronic Resource
    Electronic Resource
    Structural organization and polymorphism of the alpha satellite DNA sequences of chromosomes 13 and 21 as revealed by pulse field gel electrophoresis (1991)
    Springer
    Human genetics 〈Berlin〉 86 (1991), S. 311-316 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary More than 30 unrelated individuals were analysed by pulse field gel electrophoresis for the alphoid centromeric sequences of chromosomes 13 and 21. These individuals had DNA patterns all different from each other and were most probably heterozygous at both loci. When several nuclear families were analysed in this manner, segregation was shown to be Mendelian, and no recombination event was detected over the 150 meioses scored in this study. Alphoid DNA sequences, therefore, constitute highly polymorphic centromeric markers, which can be used in linkage analysis for loci close to the centromeres of chromosomes 13 and 21.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00202418
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Regulation of the maizerab17 gene promoter in transgenic heterologous systems (1991)
    Springer
    Plant molecular biology 17 (1991), S. 985-993 
    Details
    ISSN: 1573-5028
    Keywords: rab17 promoter ; abscisic acid ; transgene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The maizerab17 gene is expressed in different plant parts in response to ABA and osmotic stress (J. Vilardellet al., Plant Mol Biol 14 (1990) 423–432). Here we demonstrate that 5′ upstream sequences of therab17 gene confer the appropriate patterns of expression on the chloramphenicol acetyl transferase (CAT) reporter gene in transgenic tobacco plants, as well as in protoplasts derived from cultured rice cells. Specifically, a CAT construct containing a large 5′ upstream fragment ofrab17 (−1330/+29) results in high levels of CAT activity in embryos, leaves and roots of transgenic plants subjected to water stress or ABA treatment. Transient expression assays in rice protoplasts transfected with CAT genes fused torab17 promoter deletions indicate that a 300 bp DNA fragment (−351/−102) is sufficient to confer ABA responsiveness upon the reporter gene. Furthermore, a 100 bp sequence (−219/−102) is capable of conferring ABA responsiveness upon a minimal promoter derived from the 35S CaMV promoter. Gel retardation experiments indicate that maize nuclear proteins bind to this fragment. This region of 100 bp contains a sequence (ACGTGGC) which has been identified as an abscisic acid response element in studies of other ABA-responsive plant genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00037138
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Regulation of the abscisic acid-responsive gene rab28 in maize viviparous mutants (1991)
    Springer
    Molecular genetics and genomics 230 (1991), S. 394-400 
    Details
    ISSN: 1617-4623
    Keywords: Maize rab gene ; Abscisic acid ; Water-stress ; viviparous mutants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have isolated a new maize gene, rab 28, that responds to abscisic acid (ABA) treatment. This gene has been characterized by determining the sequence of the cDNA and corresponding genomic copy, and by mapping the start site of its transcript. The rab 28 gene encodes a protein of predicted molecular weight 27713 Da which shows strong homology with the Lea D-34 protein identified in cotton. The proximal promoter region contains the conserved ABA-response element, CACGTGG, reported in other plant genes to be responsible for ABA induction. rab 28 mRNA has been identified as ABA-inducible in embryos and young leaves. It is also induced by water-stress in leaves of wild-type plants. Regulation of the rab 28 gene was studied in maize viviparous mutants. The results obtained with the ABA-insensitive vp1 mutant show that rab 28 transcripts do not accumulate to a significant level during embryogenesis. Surprisingly, induction of rab 28 mRNA can be achieved in young embryos by exogenous ABA treatment. Moreover, water-stressed or ABA-treated seedlings of vp1 contain significant levels of rab 28 mRNA which is not detectable in well-watered seedlings. Regulation of the rab 28 gene in excised young embryos of ABA-deficient vp2 mutants, in which influences of the maternal environment are absent, closely resembles that found in non-mutant excised young embryos. The significance of these results is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00280296
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Vertical distribution of epipelagic siphonophores at the confluence between Benguela waters and the Angola Current over 48 hours (1991)
    Springer
    Hydrobiologia 216-217 (1991), S. 355-362 
    Details
    ISSN: 1573-5117
    Keywords: Siphonophora ; vertical distribution ; diel cycle ; Southeast Atlantic
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The diel distribution of epipelagic siphonophores at a station off northern Namibia (18°00′ S 10°30′ E) was studied. This area is characterized by the mixing of surface waters of the Angola Current with the waters of the northernmost Benguela region. During the sampling period, the continuous flow of the Angola Current gave rise to a marked thermocline at ca 30 m depth. In order to study the diel vertical distribution patterns of epipelagic species under these hydrographic conditions, narrow depth horizons in the top 200 m of the water column were sampled repeatedly over a 48 h period. Thirty-four species were collected. Sphaeronectes gracilis and Chelophyes appendiculata predominated. Increasing numbers of species and individuals were observed on the second day of sampling, particularly in the layers above the thermocline. Only a few species showed an upward nocturnal migration that crossed the boundary between 2 water masses. The differences in the depth distributions of the various species were related to 3 distinct phenomena: the existence of 2 separate siphonophore populations associated with each of the 2 water masses; vertical migration by the most abundant species, which were able to cross the thermocline; and differences in patch size for the most epipelagic species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00026486
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    Paper (German National Licenses)
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