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  • Blackwell Publishing Ltd  (3)
  • Springer  (1)
  • 1975-1979  (4)
  • 1925-1929
  • 1975  (4)
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  • 1975-1979  (4)
  • 1925-1929
Year
  • 1
    Electronic Resource
    Electronic Resource
    Protein Synthesis in Plant Microsomal System (1975)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 34 (1975), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: An active microsomal system from 48-h germinating seeds of Vigna sinensis (L.) Savi has now been developed. It can incorporate amino acids into protein under both in vitro and in vivo conditions, provided dithiothreitol (a protective reagent for SH groups) and phenylthiourea (an inhibitor of phenol oxidase) are present in the buffer system for extraction; and provided the assay mixture contains added dithiothreitol.The system consists of microsomes or ribosomes, tRNA or pH 5 fraction and 20 natural amino acids, ATP and an ATP-generating system and GTP with requirement for Mg ions. The cell fractions possess aminoacyl-RNA synthetase activity as indicated by the aminoacylhydroxamate formation. Microsomal synthesis is stimulated by exogenous tRNA from Escheriehia coli or rat liver and sensitive to various inhibitors such as cyclo-heximide, chloramphenicol, fusidic acid. The ribosomal transfer reaction has absolute dependence on the microsomal wash, on the crude enzyme from the same participate source, and on a synthetic messenger. It is greatly suppressed by fusidic acid and by cycloheximide.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1975.tb01860.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Ribonucleic Acid Synthesis in Plant Mitochondria: Effects of Some Inhibitors and Cyclic Nucleotides (1975)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 35 (1975), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Mitochondria isolated from 48-h germinating Vigna sinensis (L.) Savi can incorporate [3H]uridine into acid-insoluble material. The incorporation is highly sensitive to rifampicin and partially so to ethidium bromide, two specific inhibitors of template function. The inhibitory effect of rifampicin can be partly counteracted by cyclic 3′:5′-AMP but not by cyclic 3′:5′-GMP, if they are allowed to interact with the synthetic system before the treatment with rifampicin. This indicates that cyclic AMP and rifampicin compete for a common site on the RNA polymerase responsible for DNA-dependent RNA synthesis. Inhibition by ethidium bromide is unaffected by prior nucleotide interaction with the system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1975.tb03893.x
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Proteins of Mitochondrial Synthesis (1975)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 35 (1975), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Proteins synthesized in vitro by mitochondria isolated from 48-h germinating seeds of Vigna sinensis (L.) Savi and incubated in the presence of 14C-labelled amino acids from Chlorella protein hydrolysate, have been found associated with nine products separable by acrylamide gel electrophoresis in the presence of sodium dodecyl sulphate. Cytoplasmic contribution to these products was practically eliminated by the use of cycloheximide. Most of the radioactivity was incorporated into proteins having molecular weights between 10,000 and 65,000 as determined by comparing their electrophoretic mobilities with those of standard, reference proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1975.tb03878.x
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Phytotoxic effects of Croton bonplandianum Baill. on weedy associates (1975)
    Springer
    Plant ecology 30 (1975), S. 157-163 
    Details
    ISSN: 1573-5052
    Keywords: Allelopathy ; Croton ; Germination ; Growth ; Inhibition ; Phytotoxicity ; Soil litter ; Weedy associates
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Both extract as well as leachate ofCroton bonplandianum leaves before or after washing are detrimental to at least six weedy associates, viz.Calotropis procera, Chrysopogon aciculatus, Crotalaria saltiana, Cynodon dactylon, Eupatorium odoratum andPolygonum orientale. By adding increasing quantities of decaying leaves to soil, phytotoxic activity is greatly enhanced against at leastCrotalaria saltiana, Cynodon dactylon andEvolvulus nummularius. Soil samples collected in and aroundCroton bonplandianum plants during active growth of the species are more toxic to weedy associates than the soil collection made after accumulation of debris ofCroton plants. The weeds that are mostly affected includeChrysopogon aciculatus, Cynodon dactylon, Eupatorium odoratum, Evolvulus nummularius andMikania cordata. Maximum amount of inhibitory compound fromCroton bonplandianum leaves are extractable in ethanol. The ethanol extract, on further analysis, leads to the finding that two active constituents are involved and that these constituents are probably related to abscisic or phaseic acid. The implications of the above results are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02389704
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    Paper (German National Licenses)
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