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  • Angiotensin I  (1)
  • Renal Tubule  (1)
  • 1970-1974  (2)
  • 1972  (2)
  • 1970
  • 1
    Electronic Resource
    Electronic Resource
    Bestimmung der Plasma-Renin-Konzentration mittels Radioimmunoassays für Angiotensin I (1972)
    Springer
    Journal of molecular medicine 50 (1972), S. 702-705 
    Details
    ISSN: 1432-1440
    Keywords: Plasma renin concentration ; radioimmunoassay ; angiotensin I ; Plasma-Renin-Konzentration ; radioimmunologischer Nachweis ; Angiotensin I
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Ein Radioimmunoassay für Angiotensin I und seine Anwendung für die Messung der Reninkonzentration im Plasma werden beschrieben. Die zur Herstellung von Angiotensinantikörpern und radioaktiv markiertem Angiotensin sowie zur Trennung von gebundenem und freiem Hormon benutzten Verfahren werden mitgeteilt. Die Empfindlichkeit der Methode erlaubt den Nachweis von zwanzig Pikogramm Angiotensin I. Zur Messung der Reninkonzentration wurde substratfreies Plasma mit Schafsubstrat im Überschuß versetzt und in Anwesenheit von Inhibitoren von „converting enzyme“ und Angiotensinasen bei 37°C inkubiert. Das gebildete Angiotensin wurde in 20 µl des proteinfreien Inkubationsgemisches bestimmt. Die initiale Geschwindigkeit der Angiotensinbildung wurde zur Berechnung der Reninkonzentration herangezogen. Als eine Einheit wurde die Reninmenge definiert, die ein Nanogramm Angiotensin I pro Stunde Inkubation bildet. Normalwerte unter kontrollierter natriumreicher und natriumarmer Diät wurden ermittelt. Die Empfindlichkeit der Methode erlaubt die Messung der Reninkonzentration im Plasma von Patienten mit primärem Aldosteronismus.
    Notes: Summary A radioimmunoassay for angiotensin I and its application to the measurement of plasma renin concentration are described. Outlined are the methods which were used to elicite antibodies against angiotensin I, to iodinate angiotensin I with iodine125, and to separate free from antibody-bound hormone. The method is sensitive enough to detect 20 picogrammes of angiotensin I. Substrate-free plasma was mixed with excess of sheep substrate. The mixture was incubated at 37°C in the presence of inhibitors of converting enzyme and angiotensinases. The generated angiotensin I was measured in 20 µl of the protein-free incubation mixture. The initial velocity of angiotensin generation was used to calculate the renin concentration. One unit was defined as the amount of renin which generates one nanogram of angiotensin per hour. Normal values of plasma renin concentration were obtained both during sodium loading and sodium depletion. The sensitivity of the method allows the measurement of plasma renin in patients with primary aldosteronism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01495529
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The tubular reabsorption ofl-cystine andl-cysteine (1972)
    Springer
    Pflügers Archiv 337 (1972), S. 277-284 
    Details
    ISSN: 1432-2013
    Keywords: Renal Tubule ; Microperfusion ; Cystine Reabsorption ; Arginine Reabsorption ; Cystinuria Pathogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In microperfusion experiments the reabsorption of14C-labelledl-cystine,l-cysteine, andl-arginine from rat proximal tubule was measured, and the transport interaction of these amino acids and some derivatives was tested. The following results were obtained: 1. l-arginine,l-lysine, andl-cysteine inhibitedl-cystine reabsorption. 2. Glycine, agmantine, and 2,6-diaminopimelic acid showed no influence onl-cystine reabsorption. 3. l-cysteine reabsorption was inhibited byl-arginine, but not by glycine. 4. l-cysteine and 2,6-diaminopimelic acid were unable to influence reabsorption ofl-arginine. From these results and some observations reported in the literature, the following concept is put forward for discussion.l-arginine,l-lysine andl-ornithine may be reabsorbed by two separate mechanisms in the proximal tubule.l-cystine may use only one of these ways. Here, it is possible thatl-cystine is transported asl-cysteine. This concept may find relevance in the explanation of the pathogenesis of cystinuria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00586645
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    Paper (German National Licenses)
    Fulltext
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