GLORIA

GEOMAR Library Ocean Research Information Access

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Plasma renin concentration  (2)
  • sarcoplasmic reticulum  (2)
  • Cystinuria Pathogenesis  (1)
  • Springer  (5)
  • American Institute of Physics (AIP)
  • Macmillan Magazines Ltd.
  • Nature Publishing Group (NPG)
  • Oxford University Press
  • 1995-1999  (2)
  • 1970-1974  (3)
  • 1955-1959
  • 1870-1879
  • 1998  (2)
  • 1972  (3)
  • 1956
  • 1871
Document type
Keywords
Publisher
  • Springer  (5)
  • American Institute of Physics (AIP)
  • Macmillan Magazines Ltd.
  • Nature Publishing Group (NPG)
  • Oxford University Press
Years
  • 1995-1999  (2)
  • 1970-1974  (3)
  • 1955-1959
  • 1870-1879
Year
  • 1998  (2)
  • 1972  (3)
  • 1956
  • 1871
  • 1996  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Bestimmung der Plasma-Renin-Konzentration mittels Radioimmunoassays für Angiotensin I (1972)
    Springer
    Journal of molecular medicine 50 (1972), S. 702-705 
    Details
    ISSN: 1432-1440
    Keywords: Plasma renin concentration ; radioimmunoassay ; angiotensin I ; Plasma-Renin-Konzentration ; radioimmunologischer Nachweis ; Angiotensin I
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Ein Radioimmunoassay für Angiotensin I und seine Anwendung für die Messung der Reninkonzentration im Plasma werden beschrieben. Die zur Herstellung von Angiotensinantikörpern und radioaktiv markiertem Angiotensin sowie zur Trennung von gebundenem und freiem Hormon benutzten Verfahren werden mitgeteilt. Die Empfindlichkeit der Methode erlaubt den Nachweis von zwanzig Pikogramm Angiotensin I. Zur Messung der Reninkonzentration wurde substratfreies Plasma mit Schafsubstrat im Überschuß versetzt und in Anwesenheit von Inhibitoren von „converting enzyme“ und Angiotensinasen bei 37°C inkubiert. Das gebildete Angiotensin wurde in 20 µl des proteinfreien Inkubationsgemisches bestimmt. Die initiale Geschwindigkeit der Angiotensinbildung wurde zur Berechnung der Reninkonzentration herangezogen. Als eine Einheit wurde die Reninmenge definiert, die ein Nanogramm Angiotensin I pro Stunde Inkubation bildet. Normalwerte unter kontrollierter natriumreicher und natriumarmer Diät wurden ermittelt. Die Empfindlichkeit der Methode erlaubt die Messung der Reninkonzentration im Plasma von Patienten mit primärem Aldosteronismus.
    Notes: Summary A radioimmunoassay for angiotensin I and its application to the measurement of plasma renin concentration are described. Outlined are the methods which were used to elicite antibodies against angiotensin I, to iodinate angiotensin I with iodine125, and to separate free from antibody-bound hormone. The method is sensitive enough to detect 20 picogrammes of angiotensin I. Substrate-free plasma was mixed with excess of sheep substrate. The mixture was incubated at 37°C in the presence of inhibitors of converting enzyme and angiotensinases. The generated angiotensin I was measured in 20 µl of the protein-free incubation mixture. The initial velocity of angiotensin generation was used to calculate the renin concentration. One unit was defined as the amount of renin which generates one nanogram of angiotensin per hour. Normal values of plasma renin concentration were obtained both during sodium loading and sodium depletion. The sensitivity of the method allows the measurement of plasma renin in patients with primary aldosteronism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01495529
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Die Plasma-Renin-Konzentration beim primären Hyperaldosteronismus unter Suppressions- und Stimulationsbedingungen (1972)
    Springer
    Journal of molecular medicine 50 (1972), S. 783-786 
    Details
    ISSN: 1432-1440
    Keywords: Primary aldosteronism ; Plasma renin concentration ; Primärer Hyperaldosteronismus ; Plasma-Renin-Konzentration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Bei 13 Patienten mit einem primären Hyperaldosteronismus wurde die Plasma-Renin-Konzentration unter Stimulations- und unter Suppressionsbedingungen gemessen. Eine hochempfindliche Methode zur Reninmessung, die sich eines Radioimmunoassays für Angiotensin I bediente, wurde verwandt. Bei jedem der Patienten ließ sich die Reninkonzentration im Plasma gut messen. Die Werte lagen hochsignifikant unter den Normbereichen, die unter denselben Bedingungen ermittelt worden waren. Am eindrücklichsten war der Unterschied zwischen normalen und pathologischen Werten unter Stimulationsbedingungen. Nach Kochsalzentzug stieg die Reninkonzentration zwar bei nahezu allen Patienten signifikant an, der Anstieg war jedoch subnormal, gemessen an dem gesunden Kontrollkollektiv. Renin ist auch beim primären Aldosteronismus im Plasma vorhanden und reagiert auf Stimulation qualitativ regelrecht, quantitativ jedoch subnormal.
    Notes: Summary Plasma renin concentration (PRC) was measured in thirteen patients with primary aldosteronism during high and low sodium intake. A highly sensitive method using a radioimmunoassay for angiotensin I was applied for the determinations of PRC. PRC was detectable in each patient. The values obtained were significantly lower than the normal ranges which were assessed under the same conditions. Most impressive was the difference between normal and pathological values during sodium deprivation. PRC rose in almost each patient after several days of dietary sodium restriction. The increase was subnormal, however, when compared with normal controls. Renin is present in plasma of patients with primary aldosteronism. It responds qualitatively normally, but quantitatively subnormally when stimulated by sodium deprivation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01490305
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    The tubular reabsorption ofl-cystine andl-cysteine (1972)
    Springer
    Pflügers Archiv 337 (1972), S. 277-284 
    Details
    ISSN: 1432-2013
    Keywords: Renal Tubule ; Microperfusion ; Cystine Reabsorption ; Arginine Reabsorption ; Cystinuria Pathogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In microperfusion experiments the reabsorption of14C-labelledl-cystine,l-cysteine, andl-arginine from rat proximal tubule was measured, and the transport interaction of these amino acids and some derivatives was tested. The following results were obtained: 1. l-arginine,l-lysine, andl-cysteine inhibitedl-cystine reabsorption. 2. Glycine, agmantine, and 2,6-diaminopimelic acid showed no influence onl-cystine reabsorption. 3. l-cysteine reabsorption was inhibited byl-arginine, but not by glycine. 4. l-cysteine and 2,6-diaminopimelic acid were unable to influence reabsorption ofl-arginine. From these results and some observations reported in the literature, the following concept is put forward for discussion.l-arginine,l-lysine andl-ornithine may be reabsorbed by two separate mechanisms in the proximal tubule.l-cystine may use only one of these ways. Here, it is possible thatl-cystine is transported asl-cysteine. This concept may find relevance in the explanation of the pathogenesis of cystinuria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00586645
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Influence of different culture conditions on sarcoplasmic reticular calcium transport in isolated neonatal rat cardiomyocytes (1998)
    Springer
    Molecular and cellular biochemistry 188 (1998), S. 177-185 
    Details
    ISSN: 1573-4919
    Keywords: neonatal rat cardiomyocyte culture ; sarcoplasmic reticulum ; phospholamban ; calcium ATPase ; calcium transport ; thyroid hormone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract This study investigates sarcoplasmic reticulum (SR) calcium-(Ca2+) transport ATPase (SERCA2a) and phospholamban (PLB) in cultured spontaneously contracting neonatal rat cardiomyocytes (CM) to ascertain the function of both SR proteins under various culture conditions. The two major SR proteins were readily detectable in cultured CM by immunofluorescent microscopy using specific anti-SERCA2 and anti-PLB antibodies. Double labeling technique revealed that PLB-positive CM also labeled with anti-SERCA2. Coexpression of SERCA2 and PLB in CM was supported by measurement of cell homogenate oxalate-supported Ca2+ uptake which was completely inhibited by thapsigargin and stimulated by protein kinase A-catalyzed phosphorylation. Under serum-free conditions, incubation of CM with the SERCA2a expression modulator 3,3′,5-triiodo-L-thyronine (100 nM, 72 h) resulted in elevated Ca2+ uptake of +33%. Specific Ca2+ uptake activity was not altered if insulin was omitted from the serum-free culture medium but total SR Ca2+ transport activity was reduced under this culture condition. The results indicate that primary culture of spontaneously contracting neonatal rat CM can be employed as a useful model system for investigating both short- and long-term mechanisms determining the Ca2+ re-uptake function of the SR under defined culture conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006850724830
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Mechanisms of thyroid hormone control over sensitivity and maximal contractile responsiveness to β-adrenergic agonists in atria (1998)
    Springer
    Molecular and cellular biochemistry 184 (1998), S. 419-426 
    Details
    ISSN: 1573-4919
    Keywords: atria ; thyroid hormones ; β-adrenergic effect ; sarcoplasmic reticulum ; phospholamban
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract This paper discusses the mechanisms of two basic effects of thyroid hormones on atrial responses to β-adrenergic agonists, i.e. increased inotropic sensitivity and decreased maximal contractile responsiveness. The increased sensitivity of atria to β-adrenergic agonists under thyroid hormones appears to be related to increases in β-adrenoceptor density and Gs/Gi protein ratio, leading to activation of Gs-mediated pathway, but suppression of Gi-mediated pathway of adenylate cyclase regulation. Therefore, the i/c concentrations of cAMP and corresponding inotropic responses achieve their maximums at lower doses of β-adrenergic agonist. Thyroid hormones also decrease the expression of phospholamban, but increase the expression of sarcoplasmic reticulum Ca+2-pump. As a result, the basal activity of sarcoplasmic reticulum Ca+2-pump increases, but its β-adrenergic activation through phosphorylation of phospholamban decreases. It is suggested that these changes are causal for decreased maximal inotropic and lusitropic responses of atria to β-adrenergic agonists.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006835213108
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...