In:
ELECTROPHORESIS, Wiley, Vol. 31, No. 19 ( 2010-10), p. 3314-3325
Abstract:
α‐1‐acid glycoprotein (AGP) is a highly heterogeneous protein that presents a vast number of isoforms (molecules of the protein differing in its peptidic and/or glycosidic moieties). In the last years, several authors have studied the potential use of AGP as a cancer biomarker. These studies focus on the correlation of different features of AGP structure ( i.e. fucosylation, antennarity) with cancer or on the total protein blood concentration. In this study, the potential of CZE‐UV and CZE‐ESI‐MS analysis of intact AGP isoforms to study the correlation of this protein with bladder cancer is shown. Samples from 16 individuals (eight healthy, eight bladder cancer) were analyzed and characterized in great detail including data on intact protein isoforms and on released glycans. The analytical data were evaluated employing different statistical techniques (ANOVA; principal component analysis, PCA; linear discriminant analysis; and partial least squares‐discriminant analysis). Statistical differences between the two groups of study were observed. The best results were obtained by linear discriminant analysis of the CZE‐ESI‐MS data for intact AGP isoforms (93.75% of correct classification). Due to MS characterization, it can be observed that differences between the samples are mainly due to higher abundance of AGP isoforms containing tri‐ and tetra‐antennary fucosylated oligosaccharides in cancer patients. The results show the great potential of CE‐MS in combination with advanced data processing for the use of intact protein isoforms as disease biomarkers.
Type of Medium:
Online Resource
ISSN:
0173-0835
,
1522-2683
DOI:
10.1002/elps.201000244
Language:
English
Publisher:
Wiley
Publication Date:
2010
detail.hit.zdb_id:
1475486-1
SSG:
12
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